5 S ribosomal RNA is contained within a 25 S ribosomal RNA that accumulates in mutants of Escherichia coli defective in processing of ribosomal RNA

Ghora, Basanta K.; Apirion, David

doi:10.1016/0022-2836(79)90234-1

Cited by 16 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of the 4S RNA and p5 rRNA cleaved in vitro from 9S RNA by partially purified RNase E suggests that this enzyme, like other procaryotic processing enzymes, generates 5' phosphoryl and 3' hydroxyl termini (58). RNase E also processes p5 rRNA from larger rRNA transcripts which contain p5 and trailer tRNA's (132) as well as p5 rRNA from 25S rRNA which contains p5 and 23S rRNA (60).…”

Section: Rnase Mmentioning

confidence: 99%

Processing of procaryotic ribonucleic acid.

Gegenheimer¹,

Apirion²

1981

Microbiological Reviews

107

View full text Add to dashboard Cite

show abstract

Section: Rnase Mmentioning

confidence: 99%

Processing of procaryotic ribonucleic acid.

Gegenheimer¹,

Apirion²

1981

Microbiological Reviews

107

View full text Add to dashboard Cite

show abstract

“…E. coli cells de¢cient in RNase III are viable but accumulate 25S and 30S rRNAs, where 25S rRNA contains contiguous 5S and 23S rRNAs [3]. The accumulation of 25S rRNA in rnc mutants can be inhibited by chloramphenicol, an antibiotic that arrests protein translation.…”

Section: Introductionmentioning

confidence: 99%

RNase III deficient Salmonella typhimurium LT2 contains intervening sequences (IVSs) in its 23S rRNA

Mattatall

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

Salmonella typhimurium LT2 contains intervening sequences (IVSs) of 90^110 nt within all its 23S rRNA that are cleaved out by RNase III, resulting in rRNA fragmentation. In order to determine the functionality of 23S rRNA that contains unexcised IVSs, we constructed an S. typhimurium RNase III (rnc) deficient strain by transducing a mini-Tn10 (rnc-14: :Tn10) from Escherichia coli K-12. The resulting strain of S. typhimurium was viable, contained IVSs within all of its 23S rRNA, and showed a growth reduction similar to that observed for the RNase III deficient strain of E. coli. These results indicate that ribosomes containing 23S rRNA in which IVSs are not excised are functional in translation, and make it unlikely that RNase III excision of IVSs from strain LT2 23S rRNA is dictated by a selective pressure to uphold the functional integrity of ribosomes. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

show abstract

“…The study of RNA processing and decay in E. coli has made extensive use of two mutants of RNase E that are temperature sensitive 8 9 24 25 and have amino acid substitutions in the S1-like domain 26 . However, the interpretation of whether specific steps require the endonucleolytic activity of RNase E has been complicated by reports that residual activity is retained at non-permissive temperatures in vivo 27 28 and the observation that for at least one of these mutants aggregation is associated with thermal inactivation in vitro (McDowall, K. J.…”

mentioning

confidence: 99%

The First Small-Molecule Inhibitors of Members of the Ribonuclease E Family

Kime

Vincent

Gendoo

et al. 2015

Sci Rep

View full text Add to dashboard Cite

The Escherichia coli endoribonuclease RNase E is central to the processing and degradation of all types of RNA and as such is a pleotropic regulator of gene expression. It is essential for growth and was one of the first examples of an endonuclease that can recognise the 5′-monophosphorylated ends of RNA thereby increasing the efficiency of many cleavages. Homologues of RNase E can be found in many bacterial families including important pathogens, but no homologues have been identified in humans or animals. RNase E represents a potential target for the development of new antibiotics to combat the growing number of bacteria that are resistant to antibiotics in use currently. Potent small molecule inhibitors that bind the active site of essential enzymes are proving to be a source of potential drug leads and tools to dissect function through chemical genetics. Here we report the use of virtual high-throughput screening to obtain small molecules predicted to bind at sites in the N-terminal catalytic half of RNase E. We show that these compounds are able to bind with specificity and inhibit catalysis of Escherichia coli and Mycobacterium tuberculosis RNase E and also inhibit the activity of RNase G, a paralogue of RNase E.

show abstract

5 S ribosomal RNA is contained within a 25 S ribosomal RNA that accumulates in mutants of Escherichia coli defective in processing of ribosomal RNA

Cited by 16 publications

References 20 publications

Processing of procaryotic ribonucleic acid.

Processing of procaryotic ribonucleic acid.

RNase III deficient Salmonella typhimurium LT2 contains intervening sequences (IVSs) in its 23S rRNA

The First Small-Molecule Inhibitors of Members of the Ribonuclease E Family

Contact Info

Product

Resources

About