1975
DOI: 10.1038/255033a0
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5′-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation

Abstract: Unmethylated reovirus and VSV mRNAs are specifically methylated to form 5'-terminal structures of the type, m-7-G(5')ppp(5')N by protein synthesising extracts prepared from wheat germ and mouse L cells. Reticulocyte mRNA also contains 5'-terminal m-7-G. MRNAs having 5'-terminal m-7-G stimulate protein synthesis in vitro. Removal of m-7-G by beta-elimination abolishes translation of the mRNAs.

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Cited by 458 publications
(201 citation statements)
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“…The m 7 G cap modulates numerous facets of mRNA metabolism, including stability (12,13), translation (14,15), and export (16). The importance of this modification is underscored by the substantial cellular machinery dedicated to its addition and removal (17,18).…”
mentioning
confidence: 99%
“…The m 7 G cap modulates numerous facets of mRNA metabolism, including stability (12,13), translation (14,15), and export (16). The importance of this modification is underscored by the substantial cellular machinery dedicated to its addition and removal (17,18).…”
mentioning
confidence: 99%
“…transcription | Saccharomyces cerevisiae | killer toxin E ukaryotic mRNA is capped at the 5′ end by a 7-methyl GMP moiety via an inverted 5′-5′ triphosphate linkage (m 7 GpppX) (1,2). The cap structure is required for mRNA stability and efficient translation.…”
mentioning
confidence: 99%
“…RNA pppG + pppC polymeras pppG-C + Hp~ [1] [5] The availability of methylated and unmethylated mRNAs from reovirus and other viruses has facilitated studies on the role of caps in mRNA function (16)(17)(18)(19). The 5'-terminal m7G appears to be important for translation, apparently as part of a recognition mechanism for initiation complex formation (20)(21)(22)(23)(24)(25)(26)(27).…”
mentioning
confidence: 99%