50-plus years of fungal viruses

Ghabrial, Said A.; Castón, José R.; Jiāng, Dàohóng; Nibert, Max L.; Suzuki, Nobuhiro

doi:10.1016/j.virol.2015.02.034

Cited by 630 publications

(594 citation statements)

References 134 publications

Supporting

Mentioning

585

Contrasting

Unclassified

Order By: Relevance

“…These genes could then be disrupted as described in this report to broaden the effectiveness of the SD strains. Both the comparative genomics approach for identifying vic genes and the multilocus vic gene disruption strategy to promote mycovirus transmission should find general applicability for enhancing the biological control potential for the growing list of pathogenic fungi/mycovirus hypovirulence systems (2,6,39).…”

Section: Discussionmentioning

confidence: 99%

“…mycovirus transmission | hypovirulence | vegetative incompatibility | non-self recognition | Cryphonectria parasitica M ycovirus infections have been reported to reduce virulence (hypovirulence) for a wide range of plant pathogenic fungi, providing potential for biological disease control (1)(2)(3)(4)(5)(6). For hypovirulence to be effective, the virulence-attenuating viruses must be efficiently transmitted from infected hypovirulent strains to uninfected virulent strains (5,7).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes

Zhang

Nuss

2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Transmission of mycoviruses that attenuate virulence (hypovirulence) of pathogenic fungi is restricted by allorecognition systems operating in their fungal hosts. We report the use of systematic molecular gene disruption and classical genetics for engineering fungal hosts with superior virus transmission capabilities. Four of five diallelic virus-restricting allorecognition [vegetative incompatibility (vic)] loci were disrupted in the chestnut blight fungus Cryphonectria parasitica using an adapted Cre-loxP recombination system that allowed excision and recycling of selectable marker genes (SMGs). SMG-free, quadruple vic mutant strains representing both allelic backgrounds of the remaining vic locus were then produced through mating. In combination, these super donor strains were able to transmit hypoviruses to strains that were heteroallelic at one or all of the virus-restricting vic loci. These results demonstrate the feasibility of modulating allorecognition to engineer pathogenic fungi for more efficient transmission of virulence-attenuating mycoviruses and enhanced biological control potential.M ycovirus infections have been reported to reduce virulence (hypovirulence) for a wide range of plant pathogenic fungi, providing potential for biological disease control (1-6). For hypovirulence to be effective, the virulence-attenuating viruses must be efficiently transmitted from infected hypovirulent strains to uninfected virulent strains (5, 7). Mycoviruses generally have evolved exclusive intracellular lifestyles (8). With very few exceptions (9), mycovirus infections cannot be initiated by exposure of uninfected hyphae to cell extracts or secretions from infected fungal isolates. Transmission is limited to intracellular mechanisms, vertical transmission through asexual spores, and horizontal transmission through anastomosis (fusion of hyphae).Horizontal mycovirus transmission to uninfected isolates of the same fungal species is complicated by nonself allorecognition genetic systems, termed "heterokaryon" or "vegetative incompatibility" (vic), which operate widely in filamentous fungi (10). Interactions between genetically distinct individuals of the same species result in an incompatible reaction that triggers localized programmed cell death (PCD), forming a line of demarcation, or barrage, along the zone of contact (10-12) and restricting cytoplasmic transmission of viruses and other cytoplasmic elements (1,(13)(14)(15).A negative correlation between vic diversity and virus transmission has been reported for several fungal hosts (1, 16), but has most extensively been demonstrated for the chestnut blight fungus Cryphonectria parasitica infected with virulence-attenuating hypoviruses (7,(17)(18)(19)(20). Genetic analyses have defined six diallelic vic genetic loci for C. parasitica (21). These loci and associated genes were recently identified at the molecular level through a comparative genomics approach (22, 23) ( Table 1). Independent gene disruption analysis of 12 genes associated with these loci (22, 23) ...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes

Zhang

Nuss

2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…mycovirus | biological control | mycophagous insect | mutualism | gemycircularvirus M ycoviruses infect fungi and replicate in fungal cells that are widespread in all major fungal groups (1). As a part of the virus world, mycoviruses may play an ecologically important role in nature (2).…”

mentioning

confidence: 99%

Fungal DNA virus infects a mycophagous insect and utilizes it as a transmission vector

Liu

Xiè

Chéng

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

150

View full text Add to dashboard Cite

Mycoviruses are usually transmitted horizontally via hyphal anastomosis and vertically via sexual/asexual spores. Previously, we reported that a gemycircularvirus, Sclerotinia sclerotiorum hypovirulenceassociated DNA virus 1 (SsHADV-1), could infect its fungal host extracellularly. Here, we discovered that SsHADV-1 could infect a mycophagous insect, Lycoriella ingenua, and use it as a transmission vector. Virus acquired by larvae feeding on colonies of a virus-infected strain of S. sclerotiorum was replicated and retained in larvae, pupae, adults, and eggs. Virus could be transmitted to insect offspring when larvae were injected with virus particles and allowed to feed on a nonhost fungus. Virus replication in insect cells was further confirmed by inoculating Spodoptera frugiperda cells with virus particles and analyzing with RT-PCR, Northern blot, immunofluorescence, and flow cytometry assays. Larvae could transmit virus once they acquired virus by feeding on virus-infected fungal colony. Offspring larvae hatched from viruliferous eggs were virus carriers and could also successfully transmit virus. Virus transmission between insect and fungus also occurred on rapeseed plants. Virus-infected isolates produced less repellent volatile substances to attract adults of L. ingenua. Furthermore, L. ingenua was easily observed on Sclerotinia lesions in rapeseed fields, and viruliferous adults were captured from fields either sprayed with a virus-infected fungal strain or nonsprayed. Our findings may facilitate the exploration of mycoviruses for control of fungal diseases and enhance our understanding of the ecology of SsHADV-1 and other newly emerging SsHADV-1-like viruses, which were recently found to be widespread in various niches including human HIV-infected blood, human and animal feces, insects, plants, and even sewage. mycovirus | biological control | mycophagous insect | mutualism | gemycircularvirus

show abstract

“…Usually, a mycovirus infection remains latent and shows no symptoms in the fungal host (Ghabrial & Suzuki, 2009). Other mycoviruses are able to cause severe lesions and impair the vegetative growth of their fungal host, causing economic losses as in the case of the cultivated mushroom Agaricus bisporus (Ghabrial & Suzuki, 2009, Ghabrial, et al, 2015. Presence of a mycovirus can also be beneficial for all the partners in the tripartite interaction of virus, fungus and plant host.…”

Section: Introductionmentioning

confidence: 99%

“…Most viruses have double stranded RNA (dsRNA) or linear positive (+) ssRNA genomes (Ghabrial & Suzuki, 2009). Unclassified linear negative ssRNAs genomes and circular ssDNA viruses have also been isolated (Liu, et al, 2014, Ghabrial, et al, 2015, Marzano & Domier, 2016. Linear (+) ssRNA mycoviruses are classified into 5 families: Alphaflexiviridiae, Gammaflexiviridae, Hypoviridae, Narnaviridae and Barnaviridae (Andrew King, 2012, Ghabrial, et al, 2015.…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanisms regulating plant infection in the rice blast fungus Magnaporthe oryzae

Otero¹

View full text Add to dashboard Cite

50-plus years of fungal viruses

Cited by 630 publications

References 134 publications

Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes

Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes

Fungal DNA virus infects a mycophagous insect and utilizes it as a transmission vector

Molecular mechanisms regulating plant infection in the rice blast fungus Magnaporthe oryzae

Contact Info

Product

Resources

About