500  μm field-of-view probe-based confocal microendoscope for large-area visualization in the gastrointestinal tract

Li, Hua; Hao, Zhengyi; Huang, Jiangfeng; Lu, Tingting; Liu, Qian; Fu, Ling

doi:10.1364/prj.431767

Cited by 11 publications

(8 citation statements)

References 45 publications

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“…Image resolution gained is normally accompanied by a reduction in the area and volume that can be sampled at that resolution [67][68][69]. Some microscopy techniques offer generous fields of view (FOVs) with poor resolution while others would trade high resolution for extensive FOVs.…”

Section: Field Of View Versus Resolutionmentioning

confidence: 99%

A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences

Okolo

2022

Biochemical Society Transactions

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In the world of bioimaging, every choice made determines the quality and content of the data collected. The choice of imaging techniques for a study could showcase or dampen expected outcomes. Synchrotron radiation is indispensable for biomedical research, driven by the need to see into biological materials and capture intricate biochemical and biophysical details at controlled environments. The same need drives correlative approaches that enable the capture of heterologous but complementary information when studying any one single target subject. Recently, the applicability of one such synchrotron technique in bioimaging, soft X-ray tomography (SXT), facilitates exploratory and basic research and is actively progressing towards filling medical and industrial needs for the rapid screening of biomaterials, reagents and processes of immediate medical significance. Soft X-ray tomography at cryogenic temperatures (cryoSXT) fills the imaging resolution gap between fluorescence microscopy (in the hundreds of nanometers but relatively accessible) and electron microscopy (few nanometers but requires extensive effort and can be difficult to access). CryoSXT currently is accessible, fully documented, can deliver 3D imaging to 25 nm resolution in a high throughput fashion, does not require laborious sample preparation procedures and can be correlated with other imaging techniques. Here, we present the current state of SXT and outline its place within the bioimaging world alongside a guided matrix that aids decision making with regards to the applicability of any given imaging technique to a particular project. Case studies where cryoSXT has facilitated a better understanding of biological processes are highlighted and future directions are discussed.

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Section: Field Of View Versus Resolutionmentioning

confidence: 99%

A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences

Okolo

2022

Biochemical Society Transactions

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“…Therefore, the imaging system's ability to detect at high speeds is necessary for achieving dynamically precise observation. The imaging methods of other micro-imaging systems, such as scanning at the proximal end of the fiber, [2,9] focusing at the distal end, [3,10,36,37] and scanning with additional elements, [38,39] severely limit the high-speed imaging capabilities of the systems.…”

Section: High-speed Imagingmentioning

confidence: 99%

High‐Speed All‐Fiber Micro‐Imaging with Large Depth of Field

Wang

Yang

Liu

et al. 2022

Laser & Photonics Reviews

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Single fiber imaging has evolved into a powerful method for detecting minute objects in narrow spaces. However, existing systems are not conducive to imaging dynamic objects at depth due to their bulky probes, time-consuming scanning acquisition methods, and transmissive illumination mode. Minimally invasive reflection mode imaging with high spatial and temporal resolution remains an open challenge. Here, a precise and high-speed imaging scheme without scanning is proposed. Multimode fiber imaging technology is incorporated into an all-fiber aberration-free precision detection system. High temporal resolution (5000 fps) detection of tiny natural scenes is experimentally realized by optimizing the approximation of the inverse transmission matrix in a simple and compact setup. The system can display the detected screen in real-time and the computational imaging with a large depth of field (1 mm) is enabled by jointly learning. The recovery results are superior compared to typical deep neural networks. The demonstrated scheme offers a new possibility for many applications, for example, microendoscopy, all-optical computing, and remote high-speed video transmission.

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“…1 While thin light-sheets allowing large field-of-view (FOV) imaging have been generated by means of various nondiffracting beams, 2,3 only the one-dimensional Airy beam allows a static implementation which avoids scanning components, 4 making it suitable for miniaturized applications. The key component that opens the door to those in-vivo applications, such as endomicroscopy 5,6 and imaging and manipulation in freely moving animals, 7,8 are high-performance micro-objectives that match in performance their bench-top equivalents with high numerical apertures. 9 Due to their large FOV relative to their diameter, a field curvature can be introduced as degree of freedom in the design of micro-objectives to fulfill the high demands on imaging performance.…”

Section: Introductionmentioning

confidence: 99%

A bi-axially accelerating Airy beam for miniaturized light-sheet microscopy

Taege

Winter

Ataman

2023

Advanced Fabrication Technologies for Micro/Nano Optics and Photonics XVI

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The use of one-dimensional Airy beams in light-sheet microscopy (LSM) allows the generation of thin lightsheets without scanning, and thus have great potential for simplifying multi-photon LSM systems. However, their uni-axial acceleration limits the maximum numerical aperture of the collection objective in order to keep both the active and inactive axes within its depth of field. This issue is particularly pronounced in potential miniaturized LSM implementations, such as those for endomicroscopy or multi-photon neural imaging in freelymoving animals using head-mounted miniscopes. To address this issue, we propose a new method to generate a bi-axially accelerating static Airy light-sheet that illuminates a thin spherical shell within the field-of-view (FOV) of a collection objective. This profile can be engineered to match both the detection objective's field curvature and FOV. Using an illumination unit that combines GRIN optics with a phase mask that is 3D-printed using 2-photon polymerization, we experimentally demonstrate the validity of the approach, and verify the analytical models.

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500 μm field-of-view probe-based confocal microendoscope for large-area visualization in the gastrointestinal tract

Cited by 11 publications

References 45 publications

A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences

A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences

High‐Speed All‐Fiber Micro‐Imaging with Large Depth of Field

A bi-axially accelerating Airy beam for miniaturized light-sheet microscopy

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