504. The stereochemistry of the bridged quaternary salts of 2,2′-bipyridyl

Homer, R. F.; Tomlinson, T. E.

doi:10.1039/jr9600002498

Cited by 121 publications

(56 citation statements)

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“…Ly a single signal at g = 2.003, with a width of about 10 The enzyme complex from M. thermophila shows an s observed by electron paramagnetic resonance specelectron paramagnetic resonance spectrum at 113 K that is )py at 77 K for CODH from M. vannielii with or nearly identical to that exhibited by the clostridial CODH ut added CO. (21,23), whereas the enzyme from M. vannielii showed no enzymes from M. vannielii and M. barkeri were tested such spectrum at 77 K. The electron paramagnetic resoieir abilities to carry out the reaction (2) also differ from that of clostridial CODH. Furthermore, elii catalyzed the exchange of 14C from [1-14C]acetyl the enzymes from M. barkeri, M. vannielii, and R. rubrum :yme A to 14CO when the experiment was performed as (2) failed to catalyze the exchange of carbon monoxide with ibed for the clostridial system (20).…”

Section: Resultsmentioning

confidence: 99%

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Purification and properties of carbon monoxide dehydrogenase from Methanococcus vannielii

DeMoll¹,

Grahame²,

Harnly³

et al. 1987

J Bacteriol

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Section: Resultsmentioning

confidence: 99%

“…Coenzyme F420 was purified from frozen cells of M. vannielii by quaternary aminoethyl and C-18 chromatography, in a manner similar to that previously described (5). 1,1'-Trimethylene-4,4'-dimethyl-2,2'-dipyridylium dibromide was synthesized by alkylation of 4,4'-dimethyl-2,2'-dipyridyl with 1,3-dibromopropane (10).…”

Section: Methodsmentioning

confidence: 99%

Purification and properties of carbon monoxide dehydrogenase from Methanococcus vannielii

DeMoll¹,

Grahame²,

Harnly³

et al. 1987

J Bacteriol

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“…N,N′-Tetramethyl-2,2′-bipyridinium dibromide (DQ 2+ ) was prepared as described by Homer and Tomilson. 17 Propyl viologen sulfonate (PVS) was also prepared following literature procedures. 18 (2,2'-Bipyridyl) ruthenium(II) chloride hexahydrate (Ru(bpy) 3 2+ , Strem Chemicals), Na 2 EDTA (GFS Chemicals), and methyl viologen dichloride hydrate (MV 2+ , Aldrich) were purchased with the highest purity available and used as received.…”

Section: Methodsmentioning

confidence: 99%

Charge Transport through a Novel Zeolite Y Membrane by a Self-Exchange Process

Lee

Dutta

2002

J. Phys. Chem. B

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The preparation of thin (∼ 1µm ), mechanically stable, and relatively pinhole-free zeolitic membranes is described. Nanocrystalline zeolite Y (100-200 nm) is used as the starting material and coated onto a porous alumina substrate. Pinholes within the membrane are filled with a positive photoresist. Upon illumination of the photoresist, only the light-exposed parts are solubilized, whereas the photoresist within the cracks is not affected, thereby sealing them. The leak properties of such membranes were tested using Rhodamine 123 dye, and levels of about 0.5% leaking as compared to the non-photoresist-coated membranes were found. Accessibility of the intrazeolitic volume was examined by ion exchange and for optimally illuminated membranes was comparable to uncoated membranes. Charge transport through the membrane by electron hopping by a self-exchange process involving bipyridinium ions was demonstrated. The electron transfer was initiated using a photochemical Ru(bpy) 3 2+ -EDTA system.

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“…3), which contrasts with the much more negative potential of the soluble viologen (E:= -430 mV NHE, pH 8). This change in the redox potential of the viologen could be explained taking into account that the covalent binding of the viologen to the protein probably implies the stabilisation of the radical cation by the framework of the protein and, therefore, a shift to more positive values in the redox potential of the viologen (Homer and Tomlinson, 1960).…”

Section: Oxidase Activity Of Modified Fnrmentioning

confidence: 99%

The Covalent Linkage of a Viologen to a Flavoprotein Reductase Transforms it into an Oxidase

Bes

Lacey

Peleato

et al. 1995

European Journal of Biochemistry

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Chemical cross-linkage of the positively charged viologen N-methyl-K-(aminopropyl)-4-4'-bipyridinium dibromide (APMV) to the enzyme ferredoxin-NADP' reductase from the cyanobacterium Anabaenu PCC 71 19 has been performed using the carbodiimide 1 -ethyl[3-(3-dimethylaminopropyl)]carbodiimide. 0.5-1 mol, depending on the preparation, is introduced for each mol enzyme. The residue involved in the covalent linkage with the viologen, Glu139, has been identified using HPLC separation of the modified proteolytic peptides and subsequent sequencing. Modification of the enzyme changes its catalytic specificity since it is able to react directly with oxygen; this is observed by a high NADPH oxidase activity, which is completely absent in the native enzyme. More important, this new enzymic activity is indicative of the intramolecular electron transfer between the natural redox cofactor FAD and the artificially introduced viologen. Electrons can also flow in the reverse direction, from the viologen to the FAD group, then to NADP', when the reaction is performed using glassy-carbon electrodes to reduce the viologen. Cyclic voltammetry experiments have shown that there is a small catalytic current between the electrode and the enzyme which is not observed in the native enzyme.

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504. The stereochemistry of the bridged quaternary salts of 2,2′-bipyridyl

Cited by 121 publications

References 0 publications

Purification and properties of carbon monoxide dehydrogenase from Methanococcus vannielii

Purification and properties of carbon monoxide dehydrogenase from Methanococcus vannielii

Charge Transport through a Novel Zeolite Y Membrane by a Self-Exchange Process

The Covalent Linkage of a Viologen to a Flavoprotein Reductase Transforms it into an Oxidase

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