Lactation has two phases: the continuous process of secretion and the mechanism of ejection which occurs only during milking. Because ejection is a transitory phase it is to be expected that within certain limits milk yield will be inversely related to the duration of milking, which, was, in fact, demonstrated many years ago with hand-milked cows(i). With machine milking the situation is different because the forces which the machine applies to the udders of all cows within a herd are the same, and thus the differences in milking rate that occur are due to differences between the cows. Under these conditions, if milking rate can be shown to affect milk yield it is a demonstration of how the physiological factors controlling milking rate can modify the expression of the inheritance of milk secretion.The machine milking-rate and milk-yield records of eighty-six first lactation heifers presented by Dodd & Foot (2) indicated that a significant relationship existed between milking rate and lactation yield, persistency and lactation length. Since these results were published milking-rate and milk-yield records have been obtained from a further 266 firstlactation heifers and an analysis of these data is given in this paper.