52. Calcium phosphates with submicron topography enhance human macrophage M2 polarization in vitro

Dijk, Lukas A. van; Utomo, Lizette; Yuan, Huipin; Gawlitta, Debby; DeBruijn, Joost

doi:10.1016/j.spinee.2020.05.155

Cited by 1 publication

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“…The mechanism underlying these outcomes is associated with the needle-shaped submicron surface topography of BCP, which promotes the differentiation of macrophages, key players in the bone-biomaterial response, towards an anti-inflammatory M2 phenotype which has been associated with a favorable pro-healing mechanism [9][10][11][12][13]. The in vitro cell culture of human macrophages (THP-1 and primary cells) on the needle-shaped surface topography showed them skewing towards the favorable pro-healing M2 phenotype within the first 4 days as compared to a needle-free surface topography used as a control [9,13]. In high-throughput screening studies of surfaces consisting of needles, it was shown than only a subset of needles' features promoted the favorable polarization towards M2 macrophages from murine and human origins [14,15].…”

Section: Introductionmentioning

confidence: 99%

Physico-Chemical Characteristics and Posterolateral Fusion Performance of Biphasic Calcium Phosphate with Submicron Needle-Shaped Surface Topography Combined with a Novel Polymer Binder

Belluomo¹,

Arriola-Alvarez

Kucko³

et al. 2022

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A biphasic calcium phosphate with submicron needle-shaped surface topography combined with a novel polyethylene glycol/polylactic acid triblock copolymer binder (BCP-EP) was investigated in this study. This study aims to evaluate the composition, degradation mechanism and bioactivity of BCP-EP in vitro, and its in vivo performance as an autograft bone graft (ABG) extender in a rabbit Posterolateral Fusion (PLF) model. The characterization of BCP-EP and its in vitro degradation products showed that the binder hydrolyses rapidly into lactic acid, lactide oligomers and unaltered PEG (polyethylene glycol) without altering the BCP granules and their characteristic submicron needle-shaped surface topography. The bioactivity of BCP-EP after immersion in SBF revealed a progressive surface mineralization. In vivo, BCP-EP was assessed in a rabbit PLF model by radiography, manual palpation, histology and histomorphometry up to 12 weeks post-implantation. Twenty skeletally mature New Zealand (NZ) White Rabbits underwent single-level intertransverse process PLF surgery at L4/5 using (1) autologous bone graft (ABG) alone or (2) by mixing in a 1:1 ratio with BCP-EP (BCP-EP/ABG). After 3 days of implantation, histology showed the BCP granules were in direct contact with tissues and cells. After 12 weeks, material resorption and mature bone formation were observed, which resulted in solid fusion between the two transverse processes, following all assessment methods. BCP-EP/ABG showed comparable fusion rates with ABG at 12 weeks, and no graft migration or adverse reaction were noted at the implantation site nor in distant organs.

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