Untitled

Еремин, А. Н.; Budnikova, L. P.; Sviridov, O. V.; Metelitsa, D. I.

doi:10.1023/a:1014362600722

Cited by 18 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation suggests that, during the incubation time, a significant amount of the enzyme is inactivated, probably denatured at the air−water interface, the ratio surface area/volume being particularly large in the drop configuration . Addition of BSA to the solution entails a decrease of the loss of enzyme, the values of the enzyme coverage, determined by droplet depletion method being then closer to the values obtained by cyclic voltammetry on both types of electrodes (Table ). The problem is however not completely eliminated as revealed by the fact that, in the absence of IgG-b coating and presence of BSA, depletion of N−HRP still occurs (Table ), albeit to a much lesser extent than in the absence of BSA ( ∼1.5 pmol cm -2 ).…”

Section: Resultssupporting

confidence: 61%

Quantitative Analysis of Catalysis and Inhibition at Horseradish Peroxidase Monolayers Immobilized on an Electrode Surface

2003

View full text Add to dashboard Cite

Out of several tries, biotinylation of the electrode surface by means of a sacrificial biotinylated immunoglobulin, followed by the anchoring of an avidin-enzyme conjugate appears as the best procedure for depositing a horseradish peroxidase (HRP) monolayer onto an electrode surface, allowing a high-yield immobilization of the enzyme within a stable and highly catalytic coating. Cyclic voltammetry is an efficient means for analyzing the catalytic reduction of H(2)O(2) at such HRP monolayer electrodes in the presence of [Os(III)(bpy)(2)pyCl](2+) (with bpy = bipyridine and py = pyridine) as a one-electron reversible cosubstrate. The odd shapes of current-potential responses, unusual bell-shaped variation of the peak or plateau current with the substrate concentration, hysteresis and trace crossing phenomena, and dependence or lack of dependence with the scan rate, can all be explained and quantitatively analyzed in the framework of the same catalysis/inhibition mechanism as previously demonstrated for homogeneous systems, taking substrate and cosubstrate mass transport of into account. According to H(2)O(2) concentration, limiting-behavior analyses based on the dominant factors or complete numerical simulation were used in the treatment of experimental data. The kinetic characteristics derived from these quantitative treatments implemented by the determination of the amount of enzyme deposited by the newly developed droplet depletion method allowed a comparison with homogeneous characteristics to be drawn. It shows that HRP remains nearly fully active once anchored on the electrode surface through the avidin-biotin linkage. On the basis of this full mechanistic and kinetic characterization, the analytical performances in H(2)O(2) detection and amperometric immunosensor applications are finally discussed.

show abstract

Section: Resultssupporting

confidence: 61%

Quantitative Analysis of Catalysis and Inhibition at Horseradish Peroxidase Monolayers Immobilized on an Electrode Surface

2003

View full text Add to dashboard Cite

show abstract

“…Recently, we have applied purified MCP to transform ferulic acid, which is an extremely abundant and widespread cinnamic acid derivative, into some oxidation products. Among them, two novel dehydrotrimers have been obtained and a dimer has shown more powerful anti-inflammation activity than the parent ferulic acid ( , ).…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of Sinapic Acid Catalyzed by Momordica charantia Peroxidase

Liu

Wan

Huang

et al. 2007

J. Agric. Food Chem.

View full text Add to dashboard Cite

Biotransformation of sinapic acid (1) with H2O2/Momordica charantia peroxidase, which exists in the widely used food M. charantia, at pH 5.0, 43 degrees C, in the presence of acetone resulted in six compounds, including four new compounds. Compound 2 was the first isolation of a new unique sinapic acid tetrameric derivate, which is formed by peroxidase catalysis in vitro. Besides 2, three other new sinapic acid dimers, 3-5 have also been isolated. Their structures were established on the basis of spectroscopic data. Compound 5 showed a stronger antioxidative activity than the parent sinapic acid (1). Compounds 4 and 5 significantly inhibited the growth of HL-60 cell at the concentration of 10-5 microl/L.

show abstract

“…For the synthesis of HP conjugates with peptides Arg Asn Trp Asp (V), Asn Trp Asp Val Tyr Lys (XIV), and Arg Asn Trp Asp Val Tyr Lys (XII) the procedure described in [18] was used. The HP concentration in the conjugates was measured spectrophotometrically using absorption coefficients A 280 = 0.73, A 403 nm = 2.275, ε = 109000 М -1 cm -1 [19]. The conjugates were stored at +4°С in the presence of the Dia plus stabilizer (Chema medika, Russia) in a 1 : 2 vol/vol conjugate-stabilizer ratio.…”

Section: Immunoassaymentioning

confidence: 99%

The synthesis of peptide fragments of high affinity receptor FcɛRI and their binding to allergen-specific immunoglobulins E

et al. 2012

View full text Add to dashboard Cite

High-affinity receptor FcepsilonR1 is a key substance which participates in IgE-dependent allergic reactions of immediate type. A minimal sequence Arg136-Asn137-Trp138-Asp139, which takes part in binding with C3 and C4 fragments of IgE was determined by methods of computer analysis. As possible analogs of FcepsilonR1 receptor, capable of binding to Fc-fragment IgE, a number of peptide compounds containing this sequence were proposed. Biological researches have shown that these peptides possess immunobiological effect and bind to IgE. Studied the ability of peptides to bind with IgE class serum antibodies specific to allergen Dermatophagoides pteronyssinus in patients with allergic bronchial asthma.

show abstract

Untitled

Cited by 18 publications

References 4 publications

Quantitative Analysis of Catalysis and Inhibition at Horseradish Peroxidase Monolayers Immobilized on an Electrode Surface

Quantitative Analysis of Catalysis and Inhibition at Horseradish Peroxidase Monolayers Immobilized on an Electrode Surface

Biotransformation of Sinapic Acid Catalyzed by Momordica charantia Peroxidase

The synthesis of peptide fragments of high affinity receptor FcɛRI and their binding to allergen-specific immunoglobulins E

Contact Info

Product

Resources

About