532‐nanometer potassium titanyl phosphate (KTP) laser‐induced expression of selective matrix metalloproteinases (MMP) in the rat larynx

Mallur, Pavan S.; Branski, Ryan C.; Amin, Milan R.

doi:10.1002/lary.21284

Cited by 17 publications

(16 citation statements)

References 15 publications

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“…34 In contrast, the pulsed potassium titanyl phosphate (KTP) laser, another commonly used laser in vocal fold surgery, is utilized for its theoretical angioselective rather than its ablative properties, and it induces tissue changes that fully return to normal by 4 weeks in a rat model. 35 The rabbit vocal fold scarring model in the current study is not intended to replicate the effect of the pulsed KTP laser.…”

Section: Discussionmentioning

confidence: 99%

A rabbit vocal fold laser scarring model for testing lamina propria tissue‐engineering therapies

Mau

2014

The Laryngoscope

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Objectives/Hypothesis To develop a vocal fold scarring model using an ablative laser in the rabbit as a platform for testing bioengineered therapies for missing or damaged lamina propria. Study Design Prospective controlled animal study. Methods An optimal laser energy level was first determined by assessing the depths of vocal fold injury created by a Holmium:YAG laser at various energy levels on fresh cadaveric rabbit larynges. The selected energy level was then used to create controlled unilateral injuries in vocal folds of New Zealand white rabbits, with the contralateral folds serving as uninjured controls. After 4 weeks, the larynges were harvested and subjected to excised-larynx phonation with high-speed imaging and immunohistochemical staining for collagen types I and III, elastin, and hyaluronic acid (HA) with quantitative histological analysis. Results 1.8 joules produced full-thickness injury of the lamina propria without extensive muscle injury. After 4 weeks, the injured vocal folds vibrated with reduced amplitude (P = 0.036) in excised-larynx phonation compared to normal vocal folds. The injured vocal folds contained a higher relative density of collagen type I (P = 0.004), higher elastin (P = 0.022), and lower HA (P = 0.030) compared to normal controls. Collagen type III was unchanged. Conclusions With its potential for higher precision of injury, this laser vocal fold scarring model may serve as an alternative to scarring produced by cold instruments for studying the effects of vocal fold lamina propria bioengineered therapies. Level of Evidence N/A.

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Section: Discussionmentioning

confidence: 99%

A rabbit vocal fold laser scarring model for testing lamina propria tissue‐engineering therapies

Mau

2014

The Laryngoscope

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“…MMP‐9, or gelatinase, has also been shown to degrade type IV and V collagen. Previously, our laboratory showed MMP‐3 regulation with the KTP on normal tissue; however, we sought to be more encompassing in the current investigation as MMP‐3 is not involved in type I collagen metabolism, which is the major component of vocal fold fibrosis. Both MMP‐1 and ‐9 gene expression increased significantly at 1 week.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, our laboratory developed an animal model to investigate the laser‐tissue dynamics of the KTP on vocal fold mucosa . We subsequently attempted to determine the biochemical sequelae of the KTP on normal vocal fold mucosa, observing regulation of matrix metalloproteinase gene expression and a brief, transient inflammatory response associated with levels of energy thought to be therapeutic by clinical standards . Similarly, Lin et al showed pulse dye laser (PDL) treatment to normal rat vocal folds results in upregulation of inflammatory and extracellular matrix modulators in vivo and in vitro .…”

Section: Introductionmentioning

confidence: 99%

The utility of the potassium titanyl phosphate laser in modulating vocal fold scar in a rat model

et al. 2013

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“…Animal studies of KTP laser–tissue interactions have offered histologic and biochemical analysis of effect on normal mucosa . Although human study of KTP laser treatment has correlated the dose and method of delivery with clinical outcome, to our knowledge there has not yet been a histopathologic assessment of KTP laser effect as it correlates with dose to identify effect on the microvasculature in clinical practice .…”

Section: Introductionmentioning

confidence: 99%

“…1,6,7 Animal studies of KTP laser-tissue interactions have offered histologic and biochemical analysis of effect on normal mucosa. 8,9 Although human study of KTP laser treatment has correlated the dose and method of delivery with clinical outcome, to our knowledge there has not yet been a histopathologic assessment of KTP laser effect as it correlates with dose to identify effect on the microvasculature in clinical practice. 7 We present histopathologic analysis of papilloma resected immediately after KTP laser treatment to correlate KTP type of energy delivery with histopathologic analysis of the microvasculature.…”

Section: Introductionmentioning

confidence: 99%

Histologic effect of the potassium‐titanyl phosphorous laser on laryngeal papilloma

Eigsti

Bayan

Robinson

et al. 2019

Laryngoscope Investig Oto

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Objectives Tissue effects occurring with potassium‐titanyl phosphorous (KTP) laser treatment are difficult to quantify due to the multiple variables that affect not only the fluence (energy delivered) but also the laser–tissue interaction. This histopathologic analysis of recurrent respiratory papilloma (RRP) removed after treatment with KTP laser therapy permits correlation of histologic effect with method of laser treatment. Methods The histopathology of RRP resected specimens in a single patient was compared following treatment with KTP laser in contact and non‐contact modes as documented with intraoperative photography and video imaging. Results Epithelial‐sparing injury selective to the microvasculature was identified on histopathologic assessment of a specimen treated with noncontact angiolysis. Highly cauterized papillomatous epithelium without identifiable vascular structures was identified on tissue removed after treatment with the KTP laser in contact mode. Conclusion The histopathologic assessment of acute KTP laser effect on papilloma permits correlation between technique of application and tissue effect. Similar assessments may be helpful to modify dosimetry for individual patients requiring repeated treatment and may also assist in refining the development of existing KTP laser treatment classification systems. Level of Evidence 4

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532‐nanometer potassium titanyl phosphate (KTP) laser‐induced expression of selective matrix metalloproteinases (MMP) in the rat larynx

Abstract: These data suggest that the KTP laser induces a modest inflammatory response, selective MMP expression, and no long-term fibrotic processes in a clinically relevant simulation.

Cited by 17 publications

References 15 publications

A rabbit vocal fold laser scarring model for testing lamina propria tissue‐engineering therapies

A rabbit vocal fold laser scarring model for testing lamina propria tissue‐engineering therapies

The utility of the potassium titanyl phosphate laser in modulating vocal fold scar in a rat model

Histologic effect of the potassium‐titanyl phosphorous laser on laryngeal papilloma

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