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Abstract

In the present study, twenty-two microsatellites was developed for Amur sturgeon (Acipenser schrenckii). All of them were polymorphic with 2 to 7 alleles per locus and the total number of alleles is 106. The expected heterozygosity (HE), observed heterozygosity (HO), polymorphic information content (PIC), Hardy-Weinberg departure value (d) and Shannon-Weiner Diversity Indices (H') ranged from 0.35 to 0.84, from 0.46 to 1, from 0.29 to 0.82, from 0.14 to 0.49, and from 0.53 to 1.89 respectively. The microsatellites described here should be useful for the research in population genetics, conservation genetics and evolution of Amur sturgeon.

Development and Characterization of Fifteen Polymorphic Microsatellite Loci for Rare and Endangered Species within Luciobarbus Heckel, 1843 Genus in the Aral Basin and Their Conservation Application

“…Of the 17 analyzed loci, 15 were polymorphic for all individuals; microsatellites were used to study the genetic structure and diversity of both L. brachycephalus and L. conocephalus. The number of alleles per locus is estimated to range from 2 to 12, namely M2044 and M4474-12, M1287-11, M4215-9, and M3444-8, with an average value of 6, in comparison with the number of alleles that fluctuate from 2 to 7 in other species [51][52][53][54]. Additionally, at two points where L. brachycephalus is usually recorded, four individuals of L. conocephalus were also found, which was confirmed by our genetic results.…”