547. A coloured maleimide and its interaction with cysteine

Spectroscopic and Kinetic Characterization of Eosin–5-Maleimide

“…Similarly, the initial reaction of the L-cysteine thiol with EM caused no change in the absorbance of EM. The amine moiety of L-cysteine is reported to make a secondary reaction with maleimides, opening the ring and forming a thiazine product (9,10). This secondary reaction with EM caused a shift in absorbance very similar to that shown in Fig.…”

Synthesis and properties of a fluorine‐containing sulfhydryl reagent for 19F NMR studies

“…Upon standing, the cysteine adduct produced a second sharp resonance slightly to lower field of the original resonance of the adduct. This is probably due to a rearrangement of the type shown in fig.2, reported to occur in other maleimide derivatives [6,7] . Noreaction was apparent between the maleimide and serine, threonine, lysine, ghrtamic acid, histidine or tryptophan under the conditions used to label 2.mercaptoethanol or cysteine.…”

Characterization and sequencing of an active-site cysteine-containing peptide from the xylanase of a thermotolerant Streptomyces

“…DAM, a yellow derivative of N-ethylmaleimide, reacts with cysteine as shown in Scheme 1 (Clark-Walker & Robinson, 1961). The reaction of the xylanase with DAM showed complete inhibition of activity at 1 or 10 mm concentration when the reaction was performed in a volume of 0.5 or 0.05 ml respectively (Fig.…”

“…Sephadex G-25 was obtained from Pharmacia (Uppsala, Sweden). DAM was synthesized according to the procedure described by Clark-Walker & Robinson (1961). All the other chemicals used were of analytical grade.…”