protein of the second complex suggests that two distinct proteins are concerned. There is no direct evidence to show whether this difference is related in any way to the original function of the protein in the cell but the difference in the invertase activity (per unit of protein) of the two preparations has already been mentioned.
AcknowledgmentThe tryptophan, tyrosine, methionine, cystine and lysine contents of the proteins of 15 herbage species, together with some histidine values, have been determined by microbiological assay, and the difficulties associated with the hydrolysis of protein in the presence of carbohydrate and lipoid materials are discussed.The values found for tryptophan, tyrosine, methionine and lysine are slightly lower than those obtained by other workers for the analysis of leaf-protein preparations. The low figures obtained for cystine are almost certainly due to its destruction during the preparation of the hydrolysates. It is suggested that there may be a wider variation in the amino-acid make-up of herbage species than has been previously observed.