[57] Dye uptake method for assay of interferon activity

Johnston, Michael D.; Finter, N. B.; Young, P. A.

doi:10.1016/0076-6879(81)78147-3

Cited by 33 publications

(14 citation statements)

References 7 publications

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“…8 -12 h later the media was changed, and cells were treated with human Apo2L/ TRAIL, FasL, or TNF (cycloheximide was used in the latter case at 0.5-2 g/ml, as indicated in the figure legends) for 18 -24 h. Cell viability was measured by neutral red uptake as described (41).…”

Section: Methodsmentioning

confidence: 99%

Molecular Determinants of Kinase Pathway Activation by Apo2 Ligand/Tumor Necrosis Factor-related Apoptosis-inducing Ligand

Varfolomeev

Maecker

Sharp

et al. 2005

Journal of Biological Chemistry

255

268

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Section: Methodsmentioning

confidence: 99%

Molecular Determinants of Kinase Pathway Activation by Apo2 Ligand/Tumor Necrosis Factor-related Apoptosis-inducing Ligand

Varfolomeev

Maecker

Sharp

et al. 2005

Journal of Biological Chemistry

255

268

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“…Twenty-four hours posttransfection, apoptosis was measured by analyzing caspase-3/caspase-7 activity using the Homogeneous ApoOne Caspase-3/7 Assay kit (Promega) according to the manufacturer's instructions. Cell viability was measured 48 h posttransfection by Neutral Red uptake as described (28).…”

Section: Methodsmentioning

confidence: 99%

Microphthalmia-Associated Transcription Factor Is a Critical Transcriptional Regulator of Melanoma Inhibitor of Apoptosis in Melanomas

Dynek¹,

Chan

Liu

et al. 2008

Cancer Research

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Melanoma inhibitor of apoptosis (ML-IAP) is a potent inhibitor of apoptosis, which is highly expressed in melanomas and likely contributes to their resistance to chemotherapeutic treatments. Herein, we show that the lineage survival oncogene microphthalmia-associated transcription factor (MITF) is a critical regulator of ML-IAP transcription in melanoma cells. The ML-IAP promoter contains two MITF consensus sites, and analysis of MITF and ML-IAP mRNA levels revealed a high correlation in melanoma tumor samples and cell lines. In reporter assays, MITF promoted a strong stimulation of transcriptional activity from the ML-IAP promoter, and MITF bound the endogenous ML-IAP promoter in melanoma cells by chromatin immunoprecipitation and electrophoretic mobility shift assay. Strikingly, small interfering RNA (siRNA)-mediated knockdown of MITF in melanoma cells led to a dramatic decrease in ML-IAP mRNA and protein levels, establishing that ML-IAP expression in melanoma cells is MITF dependent. Additionally, cyclic AMP-mediated induction of MITF expression in melanocytes resulted in increased ML-IAP expression, suggesting that melanocytes can express ML-IAP when MITF levels are heightened. Disruption of MITF by siRNA led to a decrease in melanoma cell viability, which could be rescued by ectopic expression of ML-IAP. Collectively, these findings implicate MITF as a major transcriptional regulator of ML-IAP expression in melanomas, and suggest that ML-IAP contributes to the prosurvival activity of MITF in melanoma progression. [Cancer Res 2008;68(9):3124-32]

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“…Interferon activity was assayed by the inhibition of cytopathic effects of sindbis virus in FL cells (Johnston et al, 1981). The ELISA assay for TNF-o~ was performed as previously described (Yamazaki et al, 1986).…”

Section: Mandterials and Methodsmentioning

confidence: 99%

Establishment of a human fibroblast cell line producing tumor necrosis factor α (KMST-6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture

Fushimi

Torigoe

Yamauchi

et al. 1998

In Vitro Cell.Dev.Biol.-Animal

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To develop a new gene therapy model for cancer, a clonal cell line (KMST-6/TNF) which produces human tumor necrosis factor alpha (hTNF-alpha) has been developed by introducing hTNF-alpha cDNA into a human immortal fibroblast cell line (KMST-6). The conditioned medium (CM) of KMST-6/TNF cells inhibited the growth of various malignant human cell lines, but not that of normal human fibroblasts. Although the growth inhibitory effects of KMST-6/TNF CM were neutralized to a considerable degree by anti-TNF-alpha antibody, its inhibitory effects were more marked than the purified human natural TNF-alpha itself in the same units, suggesting that KMST-6/TNF CM contains some growth inhibitory substances other than TNF-alpha. However, interferons alpha, beta, and gamma were undetectable in the KMST-6/TNF CM.

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[57] Dye uptake method for assay of interferon activity

Cited by 33 publications

References 7 publications

Molecular Determinants of Kinase Pathway Activation by Apo2 Ligand/Tumor Necrosis Factor-related Apoptosis-inducing Ligand

Molecular Determinants of Kinase Pathway Activation by Apo2 Ligand/Tumor Necrosis Factor-related Apoptosis-inducing Ligand

Microphthalmia-Associated Transcription Factor Is a Critical Transcriptional Regulator of Melanoma Inhibitor of Apoptosis in Melanomas

Establishment of a human fibroblast cell line producing tumor necrosis factor α (KMST-6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture

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