[6]-Gingerol Induces Electrogenic Sodium Absorption in the Rat Colon via the Capsaicin Receptor TRPV1

Abstract

Summary[6]-Gingerol possesses a variety of beneficial pharmacological and therapeutic properties, including anti-carcinogenic, anti-inflammatory, and anti-emetic activities. Although [6]-gingerol is known to regulate the contraction of the intestine, its effect on intestinal ion transport is unclear. The aim of this study was to examine the role of [6]-gingerol in the regulation of electrogenic ion transport in the rat intestine by measuring the transmural potential difference (DPD).[6]-Gingerol induced significant positive DPD when administered to the serosal but not mucosal side of the colon, ileum, and jejunum; the highest effect was detected in the colon at a concentration of 10 mm.[6]-Gingerol-induced increase in DPD was suppressed by ouabain, an inhibitor of Na 1 /K 1 -ATPase, whereas no effect was observed in response to bumetanide, an inhibitor of the Na2 co-transporter. In addition, DPD induction by [6]-gingerol was greatly diminished by capsazepine, an inhibitor of the capsaicin receptor TRPV1. These results suggest that [6]-gingerol induced the electrogenic absorption of sodium in the rat colon via TRPV1.

Allicin Induces Electrogenic Secretion of Chloride and Bicarbonate Ions in Rat Colon via the TRPA1 Receptor

“…Measurement of transmural potential in a preparation of rat intestinal sacs. As our previously reported method (14), a 5-cm section of the intestine comprising the jejunum, ileum, and colon was briefly removed and tied off with a thread. For measuring DPD, the intestinal sac was connected to a silicon tube (outer diameter, 4 mm) and filled with a standard buffer (119 mm NaCl, 21 mm NaHCO3, 2.4 mm K2HPO4, 0.6 mm KH2PO4, 1.2 mm MgCl2, 1.2 mm CaCl2, and 10 mm glucose).…”

[6]-Gingerol Induces Amiloride-Sensitive Sodium Absorption in the Rat Colon via the Capsaicin Receptor TRPV1 in Colonic Mucosa

“…1C). Our previous study reported that colonic transmural DPD evoked by [6]-gingerol was mostly due to ouabain-sensitive electrogenic sodium absorption (10). It is well known that sodium is absorbed via amiloride-sensitive sodium channels in the apical membrane of the colonic epithelium (16).…”

“…Although [6]-gingerol has been shown to regulate contractions of gastrointestinal parts, it was unclear whether these effects are related to its effect on gastrointestinal ion transport. Recently, our group demonstrated that the serosal administration of [6]-gingerol stimulated electrogenic sodium absorption in the rat colon via the capsaicin receptor, transient receptor potential (TRP) cation channel subfamily V member 1 (TRPV1), but did not influence bumetanide-sensitive colonic electrogenic chloride or potassium secretion (10). TRPV1 is a nonselective cation channel expressed mainly in nociceptive sensory neurons and is responsible for the detection and regulation of body temperature (11).…”

“…Matsumoto et al observed that TRPV1 was localized at nerve fibers E-mail: ytsuchiya@tojo.ac.jp both in Meissner's plexus extending to the batholateral side of the mucosal cells and at Auerbach's plexus of the muscular layer in the mouse colon (15). In our previous study, intact colon tissue containing mucosal, submucosal, and muscle layer was used in the experiments (10). Thus, our previous results suggested at least two possibilities for the stimulation of electrogenic sodium absorption by [6]-gingerol.…”

Effects of Salmon Nasal Cartilage Proteoglycan on Plasma Glucose Concentration and Active Glucose Transport in the Small Intestine

“…To measure sodium-dependent glucose transport, we measured the transmural potential difference (DPD) following our previous method (10). DPD was continuously measured by connecting calomel half-cells to the mucosal and serosal solution by means of a 2% agar bridge, and was recorded using a high-sensitivity DC chart recorder (056-1001, Hitachi, Tokyo) (11).…”