6. Polysaccharides of baker's yeast. Part IV. Mannan

Peat, Stanley; Whelan, W. J.; Edwards, Thomas E.

doi:10.1039/jr9610000029

Cited by 278 publications

(149 citation statements)

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“…The experiment described here indicated that (1 + 6)-a-linked linear oligosaccharides of mannose made up the immunological determinant. Peat et al (1961) reported that manno-oligosaccharides containing only (1 + 6)-a linkages were obtained from the mannan of S. cerevisiae by partial acid hydrolysis, and the optical rotations of the oligomers, tri-, tetra-and pentasaccharides, were + 68", + 70" and + 67.5" (in water), respectively. In our experiment, the optical rotations of (1 -+ 6)-linked manno-tri-, tetra-and pentasaccharides were + 64.7", + 70.8" and + 71 'O", respectively.…”

Section: Discussionmentioning

confidence: 99%

Studies on Fungal Polysaccharides: The Immunological Determinant of the Serologically Active Substances from Absidia cylindrospora, Mucor hiemalis and Rhizopus nigricans

Miyazaki

Hayashi

Ohshima

et al. 1979

Journal of General Microbiology

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Six manno-oligosaccharides were isolated from the partial acid hydrolysate of pronasetreated extracellular glycan (pt-ACE-11) of Absidia cylindrospora by gel filtration on Bio Gel P-2 and descending paper chromatography. The pentasaccharide and the higher molecular weight oligosaccharides significantly inhibited the precipitation reaction of intracellular glycans from Absidia cylindrospora (ACI-B), Mucor hiemalis (MhI-B) and Rhizopus nigricans (RnI-B) with anti-ACE serum. Structural analysis showed that these six oligosaccharides were composed of linear ( 1 + 6)-a-linked mannopyranosyl residues. INTRODUCTIONPreviously (Hayashi et al., 1978a, b) we have described the isolation, purification and partial characterization of the serologically active fucomannan-peptide of Absidia cylindrospora, a member of the Mucorales. Rabbit antiserum (anti-ACE serum) against the extracellular non-dialysable fraction of A. cylindrospora reacted not only with this fucomannanpeptide but also with antigenic fractions from four Mucor spp. and Rhizopus nigricans. However, anti-ACE serum did not cross-react with the purified mannans of Candida albicans and Saccharomyces cerevisiae, so it would appear that the chemical structure of the serologically active determinant might be different from those of the mannans of C. albicans and S. cerevisiae.In the present paper, the purification of six manno-oligosaccharides and the results of precipitation inhibition tests with these oligosaccharides are described. The common determinant in the serologically active substances of A. cylindrospora, M. hiemalis and R. nigricans is discussed. METHODSAnalytical methods. Quantitative analyses of carbohydrate, protein and phosphate were performed by the methods of Dubois et al. (1956( ), Lowry et al. (1951 and of Fiske & Subbarow (1925), respectively, using mannose, bovine serum albumin and KHzPOB as the standards.For paper and thin-layer chromatography (t.1.c.) the following solvent systems were used: A, ethyl acetate/pyridine/acetic acid/water (5: 5: 1 : 3, by vol.); By 1-butanol/ethanol/water (3 : 1 : 1 , by vol.). Carbohydrates on paper and thin-layer chromatograms were detected with the alkaline silver nitrate reagent (Trevelyan et al., 1950) or the periodate-benzidine reagent (Cifonelli & Smith, 1954). (The use of benzidine in the UK is prohibited under the Carcinogenic Substances Regulations, 1968). Gas-liquid chromatography (g.1.c.) was performed using a Shimadzu GC-6A chromatograph (Shimadzu, Tokyo, Japan) as described previously (Hayashi et al., 1978a). A glass column packed with 3 % (w/w) ECNSS-M on Chromosorb W (AW-DMCS, 60 to 80 mesh) was used for identification of the partially methylated alditol acetates. G. T. M I Y A Z A K I A N D OTHERScolumn packed with 3 % (w/w) silicone OV-225 on Chromosorb W (AW-DMCS, 80 to 100 mesh) at 170 "C and operated at an ionization voltage of 70 eV, a trap current of 60 ,uA and an ion source at 310 "C. Infrared spectra were recorded with a Hitachi-215 spectrometer. Preparation of ACI-By MhI-B and RnI-B.The s...

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Section: Discussionmentioning

confidence: 99%

Studies on Fungal Polysaccharides: The Immunological Determinant of the Serologically Active Substances from Absidia cylindrospora, Mucor hiemalis and Rhizopus nigricans

Miyazaki

Hayashi

Ohshima

et al. 1979

Journal of General Microbiology

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“…A cell-wall mannan fraction (M) was extracted and purified as reported by Peat et al (1961) A glucomannoprotein complex (GMP), an alkali-extracted mannan preparation (M-alk) and an alkali-acid insoluble glucan ("glucan ghosts", GG) were prepared as described by Ausiello et al (1 986) and Cassone et al (1 98 1). The crude chemical composition of these fractions has been reported by Ausiello et al (1986) and Scaringi et al (1988).…”

Section: Cell-wall Fractionsmentioning

confidence: 99%

Production and characterisation of a monoclonal antibody to a cell-surface, glucomannoprotein constituent of Candida albicans and other pathogenic Candida species

Cassone

Torosantucci

Boccanera

et al. 1988

Journal of Medical Microbiology

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“…at 68-70' for I hr. Mannans were prepared by the method of Peat, Whelan & Edwards (1961). Also, antigenic material was recovered from the cell-free supernatant fluid of a 3-day culture at 25" in Difco yeast carbon medium plus glucose.…”

Section: I94 I Campbell and R H Gilmourmentioning

confidence: 99%