6-Shogaol mediated ROS production and apoptosis via endoplasmic reticulum and mitochondrial pathways in human endometrial carcinoma Ishikawa cells

Ma, Run-Hui; Ni, Zhi‐Jing; Zhang, Fan; Zhang, Yuanyuan; Liu, Miaomiao; Thakur, Kiran; Zhang, Jianguo; Wang, Shaoyun; Wei, Zhao‐Jun

doi:10.1016/j.jff.2020.104178

Cited by 30 publications

(11 citation statements)

References 41 publications

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“…Cells were incubated with different concentrations of isorhamnetin for 24 h. After MTT (3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide salt) treatment, the absorbance values were measured at the detection wavelengths of 490 nm and 630 nm and a full-wavelength enzyme marker (TS100, Nikon, Tokyo, Japan) was used for calculation [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…Ishikawa cells at the logarithmic proliferation stage were transferred (1 × 10 5 cells/mL) to 6-well plates for the test [ 22 ]. Each group was repeated 3 times and incubated overnight in a constant temperature incubator.…”

Section: Methodsmentioning

confidence: 99%

“…Paraffin sections were dewaxed and rehydrated, which was then followed by false-positive removal, serum blocking, primary antibody addition, secondary antibody addition, DAB color development, nuclei re-staining, and microscopic examination (Leica, Wetzlar, Germany). Three fields of view for each sample were randomly selected for photographic recording [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

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Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo

Zhang

et al. 2022

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Endometrial cancer (EC) is a very common female cancer which has attracted more and more attention. According to the individual patient’s condition, the current treatment of EC patients is mainly based on surgery, which is supplemented by chemotherapy, radiotherapy, and endocrine intervention. However, these existing treatment strategies also have some inevitable limitations. Therefore, it is particularly important to find an active ingredient with low toxicity and a high safety profile against EC. Isorhamnetin is a flavonoid known to be present in a variety of plants, such as sea buckthorn, dry willow, and wolfberry. In recent years, the anti-tumor effects of isorhamnetin have been reported. In our study, isorhamnetin was shown to induce apoptosis in Ishikawa cells by inducing the endogenous mitochondrial apoptotic pathway and exogenous death receptor pathway, promoting the endoplasmic reticulum stress-related pathway, and activating the corresponding markers of UPR response. In addition, isorhamnetin affected the expression of MMP2 and MMP9-related proteins in vitro and in vivo and eventually repressed metastasis. Therefore, isorhamnetin can be used as a promising medicinal material for the treatment of EC.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo

Zhang

et al. 2022

Foods

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“…The specific experimental steps were carried out according to the instructions using reaction system of 20 μl in triplicates. The

{2}^{-\mathrm{\Delta \Delta }{C}_{{\rm{t}}}}

method was used for the relative expression analysis and the data obtained were normalized using Actin3 for mRNA expression (Ma et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Hydrogen sulfide treatment retrieves the inhibition of growth and development characteristics in silkworm (Bombyx mori) via phosphoacetyl glucosamine mutase gene knock down

Zhang

Cao

et al. 2022

Arch Insect Biochem Physiol

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Phosphoacetyl glucosamine mutase (PGM) is the key gene for glycolysis of important metabolic pathways in silkworm, and H2S (7.5 μM) can promote the growth and development of silkworm. Herein, we used body cavity injection of small‐interfering RNA (siRNA) to interfere with the PGM gene in H2S‐treated silkworms. After RNA interference (RNAi), we investigated the growth and development of the silkworm. H2S treatment could significantly recover the inhibition of body weight, cocoon weight, cocoon shell weight, and cocoon shell ratio by knocking down PGM gene in silkworm, without significant effects on eggs laying and production, and then analyzed the mRNA expression of PGM gene. The interference of siRNA significantly decreased the expression of targeted PGM gene and was concentrated in 48 h followed by gradual recovery. Three interference fragments also showed different interference effects, and siRNA of PGM‐3 exerted the highest interference effect to the target gene expression. Fat body had the highest mRNA expression of PGM gene, and the best interference effect was observed after siRNA injection. The results showed that the gene based on H2S treatment may have an important impact on the growth and development of silkworm by affecting its metabolic pathway.

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“…Red shapes = proteins with increased activity or expression; lightning bolt = proteins harbouring mutations/translocations; orange = oxidised proteins; light green = reduced proteins. ↑ ROS by reducing GSH [245,246] Note: the list is not exhaustive. ∆ΨM, mitochondrial membrane potential; TRX, thioredoxin; GRX, TXNRD; thioredoxin reductase; ↑, increase.…”

Section: The Modulation Of Ros To Target All 71 Pro-oxidant Therapiesmentioning

confidence: 99%

Reactive Oxygen Species in Acute Lymphoblastic Leukaemia: Reducing Radicals to Refine Responses

et al. 2021

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Acute lymphoblastic leukaemia (ALL) is the most common cancer diagnosed in children and adolescents. Approximately 70% of patients survive >5-years following diagnosis, however, for those that fail upfront therapies, survival is poor. Reactive oxygen species (ROS) are elevated in a range of cancers and are emerging as significant contributors to the leukaemogenesis of ALL. ROS modulate the function of signalling proteins through oxidation of cysteine residues, as well as promote genomic instability by damaging DNA, to promote chemotherapy resistance. Current therapeutic approaches exploit the pro-oxidant intracellular environment of malignant B and T lymphoblasts to cause irreversible DNA damage and cell death, however these strategies impact normal haematopoiesis and lead to long lasting side-effects. Therapies suppressing ROS production, especially those targeting ROS producing enzymes such as the NADPH oxidases (NOXs), are emerging alternatives to treat cancers and may be exploited to improve the ALL treatment. Here, we discuss the roles that ROS play in normal haematopoiesis and in ALL. We explore the molecular mechanisms underpinning overproduction of ROS in ALL, and their roles in disease progression and drug resistance. Finally, we examine strategies to target ROS production, with a specific focus on the NOX enzymes, to improve the treatment of ALL.

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6-Shogaol mediated ROS production and apoptosis via endoplasmic reticulum and mitochondrial pathways in human endometrial carcinoma Ishikawa cells

Cited by 30 publications

References 41 publications

Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo

Isorhamnetin Induces Apoptosis and Suppresses Metastasis of Human Endometrial Carcinoma Ishikawa Cells via Endoplasmic Reticulum Stress Promotion and Matrix Metalloproteinase-2/9 Inhibition In Vitro and In Vivo

Hydrogen sulfide treatment retrieves the inhibition of growth and development characteristics in silkworm (Bombyx mori) via phosphoacetyl glucosamine mutase gene knock down

Reactive Oxygen Species in Acute Lymphoblastic Leukaemia: Reducing Radicals to Refine Responses

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