607 Measurement of skin pigmentation using a chromameter in a 3-dimensional epidermal model containing functional melanocytes

Bachelor, Michael; Breyfogle, B.; Armento, A.; Klausner, Mitchell

doi:10.1016/j.jid.2016.02.647

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By Day 16, the cells have differentiated, melanin has been produced and transported within melanosomes to the keratinocytes. As expected, the Black donor tissue model contains more melanin than either the Asian or the Caucasian donor tissue models [37]. Surprisingly, the absorption coefficient and the index of refraction of the Black donor tissue model are both smaller than those of the Asian donor tissue model.…”

Section: Discussionsupporting

confidence: 68%

“…As the tissues develop over the course of three weeks, they form a multilayer, highly differentiated system with varying amounts of melanin depending on the donor type [36]. According to the manufacturer, after 14 days, the Asian derived tissue contains 20 μg of melanin, the Black derived tissue 48 μg, and the Caucasian derived tissue 10 μg [37].…”

Section: Human 3d Skin Tissue Modelsmentioning

confidence: 99%

See 1 more Smart Citation

Terahertz spectroscopy of human skin tissue models with different melanin content

Peralta¹,

Lipscomb²,

Wilmink³

et al. 2019

Biomed. Opt. Express

View full text Add to dashboard Cite

Terahertz imaging has been proposed for burns and skin cancer identification. However, the role of melanocytes, melanosomes, melanin content and distribution in determining the terahertz optical properties of human skin has not been investigated. We use terahertz time domain spectroscopy to measure the optical properties of in vitro pigmented human skin tissue models from Asian, Black, and Caucasian donors. Spectra were collected at various time intervals and used to extract the absorption coefficient and index of refraction at terahertz frequencies. Our results indicate that the degree of cell differentiation and type of donor both contribute to the measured terahertz optical properties.

show abstract

Section: Discussionsupporting

confidence: 68%

Section: Human 3d Skin Tissue Modelsmentioning

confidence: 99%

Terahertz spectroscopy of human skin tissue models with different melanin content

Peralta¹,

Lipscomb²,

Wilmink³

et al. 2019

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…Quantification of the melanin content of ex vivo skin samples was achieved using the SOLVABLE TM melanin assay, as described by Bachelor et al in a modification of the method of Costin and Raabe. 13,14 Melanin quantification was done in ex vivo skin models, which were harvested at days 6 and 9 of treatment. Data were obtained as ng melanin per mg protein and untreated samples were set equal to one.…”

Section: Melanin Quantificationmentioning

confidence: 99%

Air pollution‐induced tanning of human skin*

et al. 2021

View full text Add to dashboard Cite

Summary Background Melanism is more frequent in animals living in polluted areas on urban–industrial sites. Given that an increasing number of people are exposed to elevated air pollution levels, it is possible that environmental pollutants affect melanogenesis in human skin. Epidemiological studies have shown that exposure to traffic‐related air pollutants such as diesel exhaust particles (DEP) is associated with more clinical signs of hyperpigmentation. However, mechanistic evidence linking DEP exposure to pigmentation has been elusive. Objectives To develop an ex␣vivo skin model to allow for repetitive topical application of relevant ambient DEP, and to provide proof of concept in humans. Methods We measured skin pigmentation, melanin and pigmentation‐associated gene expression, and evaluated oxidative stress. Results Repetitive exposure of ex␣vivo skin to DEP at nontoxic concentrations increased skin pigmentation. This increase was visible to the naked eye, time dependent, and associated with an increase in melanin content and the transcription of genes involved in de novo melanin synthesis. Similarly, in healthy participants (n = 76), repetitive topical application of DEP at nontoxic concentrations increased skin pigmentation. DEP‐induced pigmentation was mediated by an oxidative stress response. After the application of DEP, epidermal antioxidants were depleted, lipid peroxidation and oxidative DNA damage were enhanced, and in a vehicle‐controlled, double‐blind clinical study DEP‐induced pigmentation was prevented by the topical application of an antioxidant mixture. Conclusions Similar to solar radiation, air pollutants cause skin tanning. As eumelanin is an antioxidant, it is proposed that this response serves to protect human skin against air pollution‐induced oxidative stress.

show abstract

607 Measurement of skin pigmentation using a chromameter in a 3-dimensional epidermal model containing functional melanocytes

Cited by 2 publications

References 0 publications

Terahertz spectroscopy of human skin tissue models with different melanin content

Terahertz spectroscopy of human skin tissue models with different melanin content

Air pollution‐induced tanning of human skin*

Contact Info

Product

Resources

About