6165

A sensitive immunochemical method for detecting 5mC in DNA fragments

“…DNA from the phage X, which is essentially devoid of 5mC, was not stained by the antibody at up to 500 ng DNA/assay. [20]. Electrophoresis of the digest was carried out in 0.8% agarose (Tris 40 mM, acetate 20 mM, Na-EDTA 2 mM) and an autoradiogram was prepared.…”

“…3. Mouse DNA digested with HpaII was also labelled with 32P using the T4 p 01 y merase technique [20] and run on 0.8% agarose gel. The T4 polymerase method enables one to obtain high specific activity labelling without significant alteration in fragment size as would happen during nick translation.…”