1998
DOI: 10.1023/a:1009862119114
|View full text |Cite
|
Sign up to set email alerts
|

Untitled

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
7
0

Year Published

2001
2001
2021
2021

Publication Types

Select...
4
2

Relationship

3
3

Authors

Journals

citations
Cited by 24 publications
(7 citation statements)
references
References 28 publications
0
7
0
Order By: Relevance
“…Plasmid pCL49 was obtained by insertion of the 2-kb plsX (truncated) plus fabH PCR product of S. enterica chromosomal DNA (amplified with primers SalS-N and SalH-C, Table II) into pCR2.1 (Invitrogen). Plasmid pORI280 that contains an erythromycin resistance gene, the origin of lactococcal replication of plasmid pWV01, and the E. coli ␤-galactosidase gene expressed under lactococcal promoter P 32 was used as the vector for gene replacement (19). Plasmid pCL58 was constructed by insertion of the 1.2-kb fabH PCR product of L. lactis chromosome DNA (amplified with primers LacH-P and LacH-C, Table II) into pCR2.1.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Plasmid pCL49 was obtained by insertion of the 2-kb plsX (truncated) plus fabH PCR product of S. enterica chromosomal DNA (amplified with primers SalS-N and SalH-C, Table II) into pCR2.1 (Invitrogen). Plasmid pORI280 that contains an erythromycin resistance gene, the origin of lactococcal replication of plasmid pWV01, and the E. coli ␤-galactosidase gene expressed under lactococcal promoter P 32 was used as the vector for gene replacement (19). Plasmid pCL58 was constructed by insertion of the 1.2-kb fabH PCR product of L. lactis chromosome DNA (amplified with primers LacH-P and LacH-C, Table II) into pCR2.1.…”
Section: Methodsmentioning
confidence: 99%
“…L. lactis cultures were grown in GM17 medium (Difco) or on GM17 agar plates (19). Antibiotics were added at the following concentrations (in g/ml): kanamycin, 50; ampicillin, 100; spectinomycin, 100; tetracycline, 12; and erythromycin, 150.…”
Section: Methodsmentioning
confidence: 99%
“…To construct the L. lactis lspA mutant strain MG1363⌬lsp, the plasmid pORI280-based chromosomal integration-excision system developed by Leenhouts et al (29,41) was used. For this purpose, two fragments of the lspA region were amplified by PCR.…”
Section: Methodsmentioning
confidence: 99%
“…Many tools are now available for genetic modification of L. lactis [38][39][40]. Plasmid vectors have been constructed for the cloning of genes.…”
Section: Genetic Engineering Of Lactococcus Lactismentioning
confidence: 99%
“…The most versatile of several different insertion vectors, one based on the replicon of pWV01 [40], will be described in some more detail here to enable deeper treatment of the creation of calculated chromosomal mutations.…”
Section: Genetic Engineering Of Lactococcus Lactismentioning
confidence: 99%