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Chen, Zhaolie; Iding, Kai; Lütkemeyer, Dirk; Lehmann, Jürgen

doi:10.1023/a:1005665530028

Cited by 22 publications

(6 citation statements)

References 8 publications

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“…From a practical point of view, serum is an ill-defined mixture of components which raises ethical and animal welfare problems regarding its harvest and collection, and there are constraints in terms of global supply versus demand [2,8,18,24,33]. Concerning cell biological perspectives, the disadvantages of serum supplementation are manifold: (1) serum batches display quantitative and qualitative variations in their composition and thus introduce a serious lot-to-lot variability; (2) serum may contain many different proteins, which can hinder product purification; (3) serum can also interfere with the effect of hormones or growth factors when studying their interaction with cells; (4) serum can be a potential source of microbial contaminants, such as fungi, bacteria, viruses, mycoplasma, and prions, posing a risk of transmission of diseases to the end product used by humans; and (5) serum is associated with high-costs [2,8,18,19,23,24,33]. Considering all these reasons, it is essential to develop a simple cell adaptation process for the culture of cells in serum-free, animal component-free, or protein-free medium conditions [18,22,26,31].…”

Section: Introductionmentioning

confidence: 99%

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Advances and Drawbacks of the Adaptation to Serum-Free Culture of CHO-K1 Cells for Monoclonal Antibody Production

Rodrigues

Costa

Henriques

et al. 2013

Appl Biochem Biotechnol

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Currently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process development. Thus, this study aims to assess the impact of SF adaptation on monoclonal antibody (mAb) production, identify the most critical steps of cell adaptation to the SF EX-CELL medium, and create basic process guidelines. The success of SF adaptation was dependent on critical steps that included accentuated cell sensitivity to common culture procedures (centrifugation, trypsinization), initial cell concentration, time given at each step of serum reduction, and, most importantly, medium supplements used to support adaptation. Indeed, only one of the five supplement combinations assessed (rhinsulin, ammonium metavanadate, nickel chloride, and stannous chloride) succeeded for the Chinese hamster ovary-K1 cell line used. This work also revealed that the chemically defined EX-CELL medium benefits mAb production in comparison with the general purpose Dulbecco's Modified Eagle's Medium, but the complete removal of serum attenuates these positive effects.

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Section: Introductionmentioning

confidence: 99%

“…Recently, there have been many efforts to develop serum-free media for the production of therapeutic proteins in mammalian cell culture [3,25,27]. This has led to the development of several commercial serum-free media specific for CHO cell culture [19].…”

Section: Introductionmentioning

confidence: 99%

Advances and Drawbacks of the Adaptation to Serum-Free Culture of CHO-K1 Cells for Monoclonal Antibody Production

Rodrigues

Costa

Henriques

et al. 2013

Appl Biochem Biotechnol

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“…Chen et al investigated the cultivation of the CHO-2DS cell line in a membrane reactor in a protein-free medium for obtaining human prothrombin [12]. It was found that bubblefree aeration was advantageous both to the growth of the cells and to the production of human prothrombin.…”

Section: Bubble-free Oxygen Gassingmentioning

confidence: 99%

Membranes in Biotechnology

Lütz¹,

Rao²,

Wandrey³

2006

Chem Eng & Technol

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Membranes have become an integral part of biotechnological processes. They have proved to be useful tools for the retention of biocatalysts in industrial biotransformations. This paper describes the development of membrane reactors and the application of membrane-based processes in biotransformations. Membrane systems for substrate dosing (e.g., aeration), catalyst retention (e.g., ultrafiltration), product separation (e.g., pervaporation), and salt removal (electrodialysis) from solutions are dealt with. The use of membranes in bioelectrochemistry is also discussed.

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“…A number of researchers have focused on the hybridization of synthetic polymers with biologically active molecules to the design of functional biomaterials for cell growth [3][4][5]. This kind of support materials could give opportunity for the cultivation of cells in serum-free medium which excludes contamination of blood proteins [6][7][8].…”

Section: Introductionmentioning

confidence: 99%