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Fielding, Burtram C.; Khan, S. Ajmal; Wang, Weizhou; Kruger, Courtney; Abrahams, Rayaana; Davison, Sean

doi:10.1023/a:1020118007875

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…Although the host specificity of the viruses and the responses they elicit may play a role in the differential costs observed, many unknown differences exist between TnSNPV and AcMNPV. For example, the TnSNPV genome is 30 kb larger than AcMNPV 35 and evolved resistance to TnSNPV in T. ni does not result in cross-resistance to AcMNPV 36 . Thus, more comparative studies on differential costs and virulence inflicted by other generalist and specialist viruses are needed to support our findings.…”

Section: Discussionmentioning

confidence: 99%

Impact Of Environmental Variation On Host Performance Differs With Pathogen Identity: Implications For Host-Pathogen Interactions In A Changing Climate

Shikano

Cory

2015

Sci Rep

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Specialist and generalist pathogens may exert different costs on their hosts; thereby altering the way hosts cope with environmental variation. We examined how pathogen-challenge alters the environmental conditions that maximize host performance by simultaneously varying temperature and nutrition (protein to carbohydrate ratio; P:C) after exposure to two baculoviruses; one that is specific to the cabbage looper, Trichoplusia ni (TnSNPV) and another that has a broad host range (AcMNPV). Virus-challenged larvae performed better on more protein-biased diets, primarily due to higher survival, whereas unchallenged larvae performed best on a balanced diet. The environmental conditions that maximized host performance differed with virus identity because TnSNPV-challenge inflicted fitness costs (reduced pupal weight and prolonged development) whereas AcMNPV-challenge did not. The performance of TnSNPV-challenged larvae rose with increasing P:C across all temperatures, whereas temperature modulated the optimal P:C in AcMNPV-challenged larvae (slightly protein-biased at 16 °C to increasingly higher P:C as temperature increased). Increasing temperature reduced pupal size, but only at more balanced P:C ratios, indicating that nutrition moderates the temperature-size rule. Our findings highlight the complex environmental interactions that can alter host performance after exposure to pathogens, which could impact the role of entomopathogens as regulators of insect populations in a changing climate.

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Section: Discussionmentioning

confidence: 99%

Impact Of Environmental Variation On Host Performance Differs With Pathogen Identity: Implications For Host-Pathogen Interactions In A Changing Climate

Shikano

Cory

2015

Sci Rep

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Identification and Characterization of a Major Early-Transcribed Gene of Trichoplusia ni Single Nucleocapsid Nucleopolyhedrovirus Using the Baculovirus Expression System

2004

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An early transcribed gene (me-53) of a South Africa strain of Trichoplusia ni single nucleocapsid nucleopolyhedrovirus (TnSNPV) was sequenced and identified. It has an open reading frame of 1146 nucleotides that encodes a protein of 382 amino acids with a molecular mass of 45.2 kDa. The deduced protein sequence alignment of 13 baculovirus ME-53s indicated that the TnSNPV ME-53 shares the highest homologies with NPV subgroup II-A Spodoptera exigua multiple and Mamestra configurata (Maco) nucleopolyhedrovirus ME-53s. The zinc finger-like motifs at the C-termini of ME-53s are highly conserved with similar patterns of cysteine positions. Upon introduction of the gene and a green fluorescent protein reporter gene into the baculovirus expression vector system, the transcriptional analysis of me-53 in two cell lines infected with the Autographa californica nucleopolyhedrovirus (AcMNPV) recombinant revealed that an early TnSNPV me-53 transcript can be detected by 1 h postinfection (hpi) until 12 hpi and a late one from 18 hpi up to 48 hpi, while early and late transcripts of the AcMNPV me-53 of the recombinant can be detected at 3 and 24 hpi, respectively. This suggested that the early and late promoters of both AcMNPV and TnSNPV me-53s were recognized in recombinant virus-infected cells. The regulatory elements of the TnSNPV me-53 promoter were also analyzed.

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Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

Rashidan¹,

Nassoury²,

Giannopoulos³

et al. 2004

BMB Reports

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Untitled

Cited by 3 publications

References 28 publications

Impact Of Environmental Variation On Host Performance Differs With Pathogen Identity: Implications For Host-Pathogen Interactions In A Changing Climate

Impact Of Environmental Variation On Host Performance Differs With Pathogen Identity: Implications For Host-Pathogen Interactions In A Changing Climate

Identification and Characterization of a Major Early-Transcribed Gene of Trichoplusia ni Single Nucleocapsid Nucleopolyhedrovirus Using the Baculovirus Expression System

Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

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