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Dörffling, Karl; Dörffling, Helga; Lesselich, G.; Luck, E.; Zimmermann, Christof; Melz, G.; Jürgens, Hans‐Ulrich

doi:10.1023/a:1002946622376

Cited by 50 publications

(5 citation statements)

References 16 publications

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“…Plants show increased freezing tolerance following exposure to low non-freezing temperatures, a phenomenon known as cold acclimation (CA) 2,3 . CA in plant cells leads to a series of physiological and biochemical changes, including increased scavenging of reactive oxygen species 4 , increased membrane stability 5 , and accumulated soluble sugars 6,7 and proline 6,8 . These responses ultimately increase freezing tolerance 9 .…”

Section: Introductionmentioning

confidence: 99%

Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

et al. 2019

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Vitis amurensis is a wild Vitis plant that can withstand extreme cold temperatures. However, the accumulation of metabolites during cold acclimation (CA) in V. amurensis remains largely unknown. In this study, plantlets of V. amurensis and V. vinifera cv. Muscat of Hamburg were treated at 4 °C for 24 and 72 h, and changes of metabolites in leaves were detected by gas chromatography coupled with time-of-flight mass spectrometry. Most of the identified metabolites, including carbohydrates, amino acids, and organic acids, accumulated in the two types of grape after CA. Galactinol, raffinose, fructose, mannose, glycine, and ascorbate were continuously induced by cold in V. amurensis, but not in Muscat of Hamburg. Twelve metabolites, including isoleucine, valine, proline, 2-oxoglutarate, and putrescine, increased in V. amurensis during CA. More galactinol, ascorbate, 2-oxoglutarate, and putrescine, accumulated in V. amurensis, but not in Muscat of Hamburg, during CA, which may be responsible for the excellent cold tolerance in V. amurensis. The expression levels of the genes encoding β-amylase (BAMY), galactinol synthase (GolS), and raffinose synthase (RafS) were evaluated by quantitative reverse transcription-PCR. The expression BAMY (VIT_02s0012 g00170) and RafS (VIT_05s0077 g00840) were primarily responsible for the accumulation of maltose and raffinose, respectively. The accumulation of galactinol was attributed to different members of GolS in the two grapes. In conclusion, these results show the inherent differences in metabolites between V. amurensis and V. vinifera under CA.

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Section: Introductionmentioning

confidence: 99%

Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

et al. 2019

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“…In addition, the physiological status of cold-stressed plants is also altered, such as transient increases in hormone levels (e.g., ABA) [8] and changes in membrane lipid composition [9]. Furthermore, the accumulation of compatible osmolytes, such as soluble sugars, betaine, and proline [10-12], and increases in the level of antioxidants [13] are also occurred. In contrast, temperate plants can withstand freezing temperatures following a period of low, but non-freezing, temperatures, a process called cold acclimation [14].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of low temperature-treated cassava apical shoots showed dynamic responses of tropical plant to cold stress

2012

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BackgroundCassava is an important tropical root crop adapted to a wide range of environmental stimuli such as drought and acid soils. Nevertheless, it is an extremely cold-sensitive tropical species. Thus far, there is limited information about gene regulation and signalling pathways related to the cold stress response in cassava. The development of microarray technology has accelerated the study of global transcription profiling under certain conditions.ResultsA 60-mer oligonucleotide microarray representing 20,840 genes was used to perform transcriptome profiling in apical shoots of cassava subjected to cold at 7°C for 0, 4 and 9 h. A total of 508 transcripts were identified as early cold-responsive genes in which 319 sequences had functional descriptions when aligned with Arabidopsis proteins. Gene ontology annotation analysis identified many cold-relevant categories, including 'Response to abiotic and biotic stimulus', 'Response to stress', 'Transcription factor activity', and 'Chloroplast'. Various stress-associated genes with a wide range of biological functions were found, such as signal transduction components (e.g., MAP kinase 4), transcription factors (TFs, e.g., RAP2.11), and reactive oxygen species (ROS) scavenging enzymes (e.g., catalase 2), as well as photosynthesis-related genes (e.g., PsaL). Seventeen major TF families including many well-studied members (e.g., AP2-EREBP) were also involved in the early response to cold stress. Meanwhile, KEGG pathway analysis uncovered many important pathways, such as 'Plant hormone signal transduction' and 'Starch and sucrose metabolism'. Furthermore, the expression changes of 32 genes under cold and other abiotic stress conditions were validated by real-time RT-PCR. Importantly, most of the tested stress-responsive genes were primarily expressed in mature leaves, stem cambia, and fibrous roots rather than apical buds and young leaves. As a response to cold stress in cassava, an increase in transcripts and enzyme activities of ROS scavenging genes and the accumulation of total soluble sugars (including sucrose and glucose) were also detected.ConclusionsThe dynamic expression changes reflect the integrative controlling and transcriptome regulation of the networks in the cold stress response of cassava. The biological processes involved in the signal perception and physiological response might shed light on the molecular mechanisms related to cold tolerance in tropical plants and provide useful candidate genes for genetic improvement.

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“…Multiple polygenic traits appear and various physiological and biochemical changes occur during the progress of acclimation and these changes often involve modifications in membrane lipid structure (Lynch and Steponkus 1987;Uemura and Steponkus 1994). Acclimation also causes an increase in the production of antioxidants, abscisic acid and compatible osmolytes such as soluble sugars and proline (Chen et al 1993;Dörffling et al 1997;Kishitani et al 1994;Koster and Lynch 1992;Lynch and Steponkus 1987;Murelli et al 1995;Nomura et al 1995;Tao et al 1998;Uemura and Steponkus 1994). The improvement of cold tolerance by acclimation involves abroad reprogramming of gene expression and metabolism.…”

Section: Introductionmentioning

confidence: 99%

Upregulation of CBF/DREB1 and cold tolerance in artificial seeds of cauliflower (Brassica oleracea var. botrytis)

Rihan

Al-Issawi

Fuller

2017

Scientia Horticulturae

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An effective protocol for cauliflower micropropagation and artificial seed production was optimised by (Rihan et al. 2012a; Rihan et al. 2012b; Rihan et al. 2011b; Rihan et al. 2012c). However, in order to be a viable alternative to traditional seeds, cauliflower artificial seeds need to show a high capacity to withstand abiotic stresses such as cold and desiccation. Therefore, in order to increase cauliflower abiotic stress tolerance, the effect of cold acclimation and drought on the cold tolerance of both cauliflower microshoots and mature plants were investigated. Moreover, the effect of cold and drought treatments on the induction of CBF/DREB1 gene regulation was tested. Both cold acclimation and drought improved the cold tolerance in both cauliflower microshoots and mature plants. However, whilst cold acclimation up-regulated CBF/DREB1 in cauliflower mature plants and microshoots, drought had the capacity only to up-regulate this gene in mature plants. Therefore, the high effect of cauliflower developmental stage on the CBF/DREB1 regulation was confirmed. Moreover, a small reduction in soil moisture had the capacity to upregulated this gene in mature cauliflower plants. The results presented in this study have an important role in the improvement of cauliflower micropropagation and the effectiveness of the artificial seed production protocol. Furthermore, the results contribute to an understanding of the cold tolerance mechanism in Brassica oleraceae var botrytis.

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Untitled

Cited by 50 publications

References 16 publications

Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

Transcriptome profiling of low temperature-treated cassava apical shoots showed dynamic responses of tropical plant to cold stress

Upregulation of CBF/DREB1 and cold tolerance in artificial seeds of cauliflower (Brassica oleracea var. botrytis)

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