Cultures of sporeforming bacteria isolated from commercially sterilized milk were examined and seasonal variations in the incidence ofB. subtilis/B. licheniformisstrains were apparent which may account for the increased rate of spoilage at 30° C. during the winter.B. subtiliswas the most common mesophilic sporeformer isolated, and under favourable conditions it produces rapid digestion of milk.Thermophilic sporeformers are frequently present, and usually produce a firm acid clot in milk held at temperatures favourable for their growth. The classification of the thermophiles isolated is uncertain and a review of the classification of the aerobic thermophilic sporeformers in general is required.Cl. thermosaccharolyticum, a gas-producing thermophile, was isolated from sterilized milk.