Untitled

Abstract

Fluoxetine hydrochloride (FH) in combination with Olanzapine (OZ) is used in treatment of depressive episodes associated with bipolar disorder. The first derivative spectrophotometric method for simultaneous determination of fluoxetine hydrochloride (FH) and olanzapine (OZ) from two component tablet dosage form has been developed and validated. Chromatographic methods viz. HPLC and HPTLC methods were reported in the literature for the simultaneous determination of FH and OZ from combined dosage forms. In the present investigation an attempt has been made to develop accurate, reproducible, rapid and cost-effective method for simultaneous determination of FH and OZ in pharmaceutical formulation. The wavelengths selected for determination of FH and OZ is 235.5nm and 296nm, respectively. The beer's law was obeyed in the concentration range of 8-80μg/mL and 2-20μg/mL for Fluoxetine and Olanzapine, respectively. The proposed methods were validated and successfully applied to the determination of FH and OZ in tablet formulations. The results suggested that the proposed procedures can be used for routine quality control of tablets containing FH and OZ.

Fluokseti̇n Ve Olanzapi̇ni̇n Eş Zamanli Spektrofotmetri̇k Tayi̇ni̇ Ve Yeşi̇l Ki̇mya Uygulamalari

“…There are also several derivative spectrophotometric methods when the literature is searched. First derivative or ratio derivative methods are used in the methods [15,16]. The active ingredient of OLZ was determined by the spectrophotometric method during sample preparation [17,18].…”