Anchovy (Engraulis japonica) is a family of engraulide in the order Clupeiformes and is widely distributed in the seas of Korea, Japan, and China. Especially in Korea, it is one of the main fishery resources and constitutes a high proportion of catch and consumption (Lee et al., 2019). Anchovy is an excellent fishery resource containing a large amount of functional ingredients such as minerals, free amino acids, betaine and nucleotide-related taste compounds, essential amino acids, polyunsaturated fatty acids, vitamins, and calcium (Kang & Choi, 2003). More importantly, anchovy contains eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in amount as high as in dark muscle fishes. EPA and DHA are essential fatty acids that are not synthesized in the body but are effective in preventing blood