670 nm photobiomodulation improves the mitochondrial redox state of diabetic wounds

Mehrvar, Shima; Mostaghimi, Soudeh; Foomani, Farnaz H.; Abroe, Betsy; Eells, Janis T.; Gopalakrishnan, Sandeep; Ranji, Mahsa

doi:10.21037/qims-20-522

Cited by 12 publications

(10 citation statements)

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“…(c) In this study, vascular images of the kidney, lung, heart, and liver were segmented using VMI. This VMI approach could be extended to other organs and pathologies, such as eye, skin wounds, and tumor as we 48,50,55 and others 56 have investigated their mitochondrial RR in the previous reports. (d) Since the technique uses optical imaging technologies, it is capable of high-resolution imaging compared to x-ray or ultrasound instruments.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, vascular images of the kidney, lung, heart, and liver were segmented using VMI. This VMI approach could be extended to other organs and pathologies, such as eye, skin wounds, and tumor as we 48 , 50 , 55 and others 56 have investigated their mitochondrial RR in the previous reports.…”

Section: Discussionmentioning

confidence: 99%

“…The VMI can also be used to explore the pathophysiology of rodent injury and treatment models. Optical metabolic imaging has been applied for several years, 26 , 40 , 48 – 52 and by adding the proposed segmentation technique, another key biomarker of injury, vascular density, would also be measured.…”

Section: Discussionmentioning

confidence: 99%

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Three-dimensional vascular and metabolic imaging using inverted autofluorescence

et al. 2021

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Significance: Three-dimensional (3D) vascular and metabolic imaging (VMI) of whole organs in rodents provides critical and important (patho)physiological information in studying animal models of vascular network.Aim: Autofluorescence metabolic imaging has been used to evaluate mitochondrial metabolites such as nicotinamide adenine dinucleotide (NADH) and flavine adenine dinucleotide (FAD). Leveraging these autofluorescence images of whole organs of rodents, we have developed a 3D vascular segmentation technique to delineate the anatomy of the vasculature as well as mitochondrial metabolic distribution.Approach: By measuring fluorescence from naturally occurring mitochondrial metabolites combined with light-absorbing properties of hemoglobin, we detected the 3D structure of the vascular tree of rodent lungs, kidneys, hearts, and livers using VMI. For lung VMI, an exogenous fluorescent dye was injected into the trachea for inflation and to separate the airways, confirming no overlap between the segmented vessels and airways. Results:The kidney vasculature from genetically engineered rats expressing endothelial-specific red fluorescent protein TdTomato confirmed a significant overlap with VMI. This approach abided by the "minimum work" hypothesis of the vascular network fitting to Murray's law. Finally, the vascular segmentation approach confirmed the vascular regression in rats, induced by ionizing radiation.Conclusions: Simultaneous vascular and metabolic information extracted from the VMI provides quantitative diagnostic markers without the confounding effects of vascular stains, fillers, or contrast agents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Three-dimensional vascular and metabolic imaging using inverted autofluorescence

et al. 2021

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“…Furthermore, PBM (904 nm, 6.23 J/cm 2 ) improved mitochondrial dynamics via modulating the expression of mitofusin 2 and dynamin-related protein 1 in type 1 diabetic rats [ 89 ], partially preventing or reversing mitochondrial dynamics dysfunction. PBM also preserved mitochondrial function and integrity in rats with neonatal hypoxic-ischemia injury, leading to significant alleviation or prevention of hypoxic-ischemia-induced brain injury [ 90 ].…”

Section: Pbm Overviewmentioning

confidence: 99%

Therapeutic Potential of Photobiomodulation for Chronic Kidney Disease

Bean

Liebert

Bicknell

et al. 2022

IJMS

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Chronic kidney disease (CKD) is a growing global public health problem. The implementation of evidence-based clinical practices only defers the development of kidney failure. Death, transplantation, or dialysis are the consequences of kidney failure, resulting in a significant burden on the health system. Hence, innovative therapeutic strategies are urgently needed due to the limitations of current interventions. Photobiomodulation (PBM), a form of non-thermal light therapy, effectively mitigates mitochondrial dysfunction, reactive oxidative stress, inflammation, and gut microbiota dysbiosis, all of which are inherent in CKD. Preliminary studies suggest the benefits of PBM in multiple diseases, including CKD. Hence, this review will provide a concise summary of the underlying action mechanisms of PBM and its potential therapeutic effects on CKD. Based on the findings, PBM may represent a novel, non-invasive and non-pharmacological therapy for CKD, although more studies are necessary before PBM can be widely recommended.

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“…NADH and FAD are autofluorescent and are measured without any exogenous labels by optical imaging techniques [22] . NADH/FAD’s ratio, also known as the redox ratio (RR), is associated with mitochondrial metabolic state and functionality [23] , [24] . Any significant imbalance in the NADH and FAD concentrations would lead to cellular dysfunction and diseases [21] .…”

Section: Introductionmentioning

confidence: 99%

Optical Metabolic Imaging of Mitochondrial Dysfunction on HADH Mutant Newborn Rat Hearts

Foomani

Jarzembowski

Mostaghimi

et al. 2021

IEEE J. Transl. Eng. Health Med.

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Background: Mitochondrial \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$\beta $ \end{document} -oxidation of fatty acids is the primary energy source for the heart and carried out by Hydroxy Acyl-CoA Dehydrogenase (HADH) encoded trifunctional protein. Mutations in the genes encoding mitochondrial proteins result in functionally defective protein complexes that contribute to energy deficiencies, excessive reactive oxygen species (ROS) production, and accumulation of damaged mitochondria. We hypothesize that a dramatic alternation in redox state and associated mitochondrial dysfunction is the underlying cause of Fatty Acid Oxidation (FAO) deficiency mutant, resulting in heart failure. Mitochondrial co-enzymes, NADH and FAD, are autofluorescent metabolic indices of cells when imaged, yield a quantitative assessment of the cells’ redox status and, in turn, that of the tissue and organ. Method: We utilized an optical cryo-imager to quantitively evaluate the three-dimensional distribution of mitochondrial redox state in newborn rats’ hearts and kidneys. Redox ratio (RR) assessment shows that mitochondrial dysfunction is extreme and could contribute to severe heart problems and eventual heart failure in the mutants. Results: Three-dimensional redox ratio (NADH/FAD) rendering, and the volumetric mean value calculations confirmed significantly decreased cardiac RR in mutants by 31.90% and 12.32%, in renal mitochondrial RR compared to wild-type control. Further, histological assessment of newborn heart myocardial tissue indicated no significant difference in myocardial tissue architecture in both control and severe (HADHA e4−/− ) conditions. Conclusion: These results demonstrate that optical imaging can accurately estimate the redox state changes in newborn rat organs. It is also apparent that the FAO mutant’s heart tissue with a low redox ratio is probably more vulnerable to cumulative damages than kidneys and fails prematurely, contributing to sudden death.

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670 nm photobiomodulation improves the mitochondrial redox state of diabetic wounds

Cited by 12 publications

References 50 publications

Three-dimensional vascular and metabolic imaging using inverted autofluorescence

Three-dimensional vascular and metabolic imaging using inverted autofluorescence

Therapeutic Potential of Photobiomodulation for Chronic Kidney Disease

Optical Metabolic Imaging of Mitochondrial Dysfunction on HADH Mutant Newborn Rat Hearts

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