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Abstract

Thunbergia grandiflora Roxb. (Mizo local name-Vakohrui or Zawngafian), commonly known as Bengal clockvine, is a long-lived, dicotyledonous plant traditionally used in the state of Mizoram, India for the treatment of cancer, diabetes, eye diseases, cuts and wounds etc. This study aimed to conduct in-vitro, in-vivo anti-inflammatory studies and HPTLC fingerprinting and quantifying the bioactive compounds from Thunbergia grandiflora Roxb. The IC 50 value of Egg albumin and BSA denaturation studies was found to be 423.59 µg/ml & 400.76 µg/ml respectively. The results showed that methanolic leaf extract of the plant 200mg/kg b.w. and 400mg/kg b.w. considerably reduced carrageenan-induced paw edema, and their effects were comparable to the standard diclofenac sodium. HPTLC analysis showed the presence of β -Sitosterol in the methanolic extract and the amount of β -sitosterol was found to be 22ng/µl. The identity of the bands of β -Sitosterol in the samples was confirmed by overlapping their UV absorption spectrum with that of the standards. The results for anti-inflammatory studies showed marked antiinflammatory activity, which was found to be concentration dependent. These results suggested the methanolic leaf extract of Thunbergia grandiflora plant of having promisinganti-inflammatory properties that could, in the future aid the pharmaceutical industry in the formulation of safe, herbal anti-inflammatory drugs. INTRODUCTION:Thunbergia grandiflora Roxb.: Inflammation is frequently associated with pain, making the site complex. Increased vascular permeability, protein denaturation, and membrane alteration are occurrences of inflammation 1 .