6S RNA modulates growth and antibiotic production in Streptomyces coelicolor

Mikulı́k, Karel; Bobek, Jan; Zídková, J.; Felsberg, Jürgen

doi:10.1007/s00253-014-5806-4

Cited by 18 publications

(28 citation statements)

References 49 publications

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“…In many organisms, 6S RNA-RNA polymerase complexes have been detected by co-immunoprecipitation or in vitro analysis (e.g. B. subtilis (14, 35, 70), L. pneumophila (29), C. burnetii (53), Aquifex aeolicus (71), and Streptomyces coelicolor (41)) supporting a general similarity to Ec 6S RNA. However, detailed understanding of interactions in other organisms is likely to require biochemical or structural analysis to uncover any potential differences from Ec 6S RNA.…”

Section: The 6s Rna-rna Polymerase Complexmentioning

confidence: 94%

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6S RNA, a Global Regulator of Transcription

Wassarman

2018

Microbiol Spectr

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6S RNA is a small RNA regulator of RNA polymerase that is present broadly throughout the bacterial kingdom. Initial functional studies in Escherichia coli revealed that 6S RNA forms a complex with RNA polymerase resulting in regulation of transcription, and cells lacking 6S RNA have altered survival phenotypes. The last decade has focused on deepening the understanding of several aspects of 6S RNA activity including: 1. Addressing questions of how broadly conserved 6S RNAs are in diverse organisms through continued identification and initial characterization of divergent 6S RNAs; 2. The nature of the 6S RNA-RNA polymerase interaction through examination of variant proteins and mutant RNAs, crosslinking approaches, and ultimately a cryo-EM structure; 3. The physiological consequences of 6S RNA function through identification of the 6S RNA-regulon and promoter features that determine 6S RNA sensitivity; 4. The mechanism and cellular impact of 6S RNA-directed synthesis of pRNAs (i.e. pRNA synthesis). Much has been learned about this unusual RNA, its mechanism of action and how it is regulated; yet, much still remains to be investigated, especially regarding potential differences in behavior of 6S RNAs in diverse bacteria.

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Section: The 6s Rna-rna Polymerase Complexmentioning

confidence: 94%

“…altered timing of sporulation and cell density changes in stationary phase) are associated specifically with the loss of Bs 6S-1 RNA (34, 36). Streptomyces coelicolor 6S RNA also accumulates with a profile similar to Ec 6S RNA and influences growth rate (41). …”

Section: S Rnas Are Widespread Throughout the Bacterial Kingdommentioning

confidence: 99%

6S RNA, a Global Regulator of Transcription

Wassarman

2018

Microbiol Spectr

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“…The 6S sRNA of S. coelicolor is capable of interacting with RNA polymerase and is a template for the transcription of short oligonucleotides. Knowledge about expression and function of 6S sRNA from antibiotic-producing Streptomyces has been limited until recently, but the deletion and complementation of the ssrS demonstrated its important role in the regulation of growth and expression of key genes for the biosynthesis of actinorhodin (Mikulik et al, 2014). These studies demonstrate the possibility of metabolic engineering of Streptomyces using ncRNAs.…”

Section: Engineering Of Streptomyces Using Ncrnasmentioning

confidence: 96%

Rapid and high-throughput construction of microbial cell-factories with regulatory noncoding RNAs

Chaudhary

Lee

2015

Biotechnology Advances

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“…Confirmation of the expected employment of small RNAs in the regulation of cell processes, including primary metabolism, developmental transitions, antibiotic production, and various stress responses is being increasingly reported (Palecková et al, 2007 ; Pánek et al, 2008 ; Swiercz et al, 2008 ; D'Alia et al, 2010 ; Mikulík et al, 2014 ). Hundreds of cis- acting asRNAs were identified using RNA sequencing in two recent studies (Vockenhuber et al, 2011 ; Moody et al, 2013 ).…”

Section: Introductionmentioning

confidence: 92%

RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts in Streptomyces

Šetinová

Šmídová

Pohl³

et al. 2018

Front. Microbiol.

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cis-Antisense RNAs (asRNAs) provide very simple and effective gene expression control due to the perfect complementarity between regulated and regulatory transcripts. In Streptomyces, the antibiotic-producing clade, the antisense control system is not yet understood, although it might direct the organism's complex development. Initial studies in Streptomyces have found a number of asRNAs. Apart from this, hundreds of mRNAs have been shown to bind RNase III, the double strand-specific endoribonuclease. In this study, we tested 17 mRNAs that have been previously co-precipitated with RNase III for antisense expression. Our RACE mapping showed that all of these mRNAs possess cognate asRNA. Additional tests for antisense expression uncovered as-adpA, as-rnc, as3983, as-sigB, as-sigH, and as-sigR RNAs. Northern blots detected the expression profiles of 18 novel transcripts. Noteworthy, we also found that only a minority of asRNAs respond to the absence of RNase III enzyme by increasing their cellular levels. Our findings suggest that antisense expression is widespread in Streptomyces, including genes of such important developmental regulators, as AdpA, RNase III, and sigma factors.

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6S RNA modulates growth and antibiotic production in Streptomyces coelicolor

Cited by 18 publications

References 49 publications

6S RNA, a Global Regulator of Transcription

6S RNA, a Global Regulator of Transcription

Rapid and high-throughput construction of microbial cell-factories with regulatory noncoding RNAs

RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts in Streptomyces

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