735 Isolation of a Human Serum Factor Affecting Uropod Bearing T Lymphocytes

Schmalstieg, Frank C.; Rudloff, Helen B.; Barnett, Don R.; Goldman, Armond S.

doi:10.1203/00006450-197804001-00740

Cited by 3 publications

(2 citation statements)

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“…HMC were counted in cytospins, according to Hogeman et al [17], who found these better reprodu cible than in blood and bone marrow smears. This is probably caused by the standardized way of prepara tion and by elimination of serum factors influencing HMC formation [18]. Although in time-lapse cinema tography a clear relationship between locomotion and HMC is confirmed [11,19], not all cells showing HMC configuration are moving [19].…”

Section: Discussionmentioning

confidence: 99%

Motility of Leukaemic Cells in Acute Leukaemia Related to Tumour Mass, Maturation and FAB Classification

Weide

Imandt²,

Langenhuijsen³

1988

Acta Haematol

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In a group of 56 patients with acute leukaemia, the relation between leukaemic cell motility and peripheral leukaemic cell count, degree of organomegaly, maturation and FAB classification was investigated. Cell motility was studied by means of directly observed motility and scoring of handmirror cells and anti-HLA-capping. The percentage of moving cells in the peripheral blood was higher than in the bone marrow. A positive correlation was found between the percentage of moving cells in the peripheral blood and the peripheral leukaemic cell count. In acute myeloid leukaemia, the percentage of moving cells in the bone marrow and the degree of organomegaly were higher in the monoblastic groups than in the myeloblastic groups. No correlation was found between directly observed motility, handmirror cells and capping. Leukaemic cell motility can play a role in the egress of leukaemic cells from the bone marrow, resulting in a higher tumour mass, thereby influencing prognosis.

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Section: Discussionmentioning

confidence: 99%

Motility of Leukaemic Cells in Acute Leukaemia Related to Tumour Mass, Maturation and FAB Classification

Weide

Imandt²,

Langenhuijsen³

1988

Acta Haematol

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“…The cytospin method is standar dized, and the cell density can be influ enced very easily by adjusting the cell con centration in the suspension. Serum fac tors, possibly influencing the HMC con figuration [16], are eliminated because washed cells are used to obtain cytospins. This might be the explanation for our ob servation that HMC counts in bone mar row and blood were comparable to each other in cytospins, while blood smears never showed an appreciable number of HMCs.…”

Section: Discussionmentioning

confidence: 99%

Handmirror Cells and Central Nervous System Relapse in Childhood Acute Lymphoblastic Leukaemia

Hogeman¹,

Huismans²,

Zantwijk³

et al. 1984

Acta Haematol

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In 53 children with acute lymphoblastic leukaemia (ALL), initial handmirror cell (HMC) count among lymphoblasts was studied in relation to the occurrence of a relapse in the central nervous system (CNS), taking into account the white blood cell count (WBC) and the immunological phenotype. The children were followed for a period limited by (1) the first CNS relapse, (2) death or (3) the closing day of this study. The median follow-up period was 30 months, range 2–106 months. HMC counts were available in bone marrow smears of 41 children and in cytospins of 35 children. Cytospins proved to give more reliable and consistent results than bone marrow smears. In the 35 ‘cytospin’ children no CNS relapses occurred in the group of 16 children with non-T-non-B-ALL and HMC counts above 10%. However, in the group of 10 children with non-T-non-B-ALL and HMC couts below 10%, and in the group of 9 children with T-ALL (HMC ≤ 11 %), 6 and 5, respectively, got a CNS relapse. The CNS relapse-free period was not significantly different between these last two groups, whereas both groups did differ significantly from the group mentioned first (p < 0.01). This was not found in bone marrow smears of 41 children, presumably because of the inaccurate counting results. A low initial HMC count in cytospins is associated with an increased risk for the occurrence of a CNS relapse in children with ALL. This prognostic factor seems to be independent of other prognostic signs such as immunological phenotype and high WBC.

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Significance of uropod-bearing lymphocytes

Schmalsteig¹,

Goldman²

1979

The Journal of Pediatrics

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735 Isolation of a Human Serum Factor Affecting Uropod Bearing T Lymphocytes

Cited by 3 publications

References 0 publications

Motility of Leukaemic Cells in Acute Leukaemia Related to Tumour Mass, Maturation and FAB Classification

Motility of Leukaemic Cells in Acute Leukaemia Related to Tumour Mass, Maturation and FAB Classification

Handmirror Cells and Central Nervous System Relapse in Childhood Acute Lymphoblastic Leukaemia

Significance of uropod-bearing lymphocytes

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