Hydrodistillation of volatile oil from the aerial parts at full flowering stage of Nepeta crassifolia Boiss. & Buhse was analysed by a combination of GC and GC-MS. Twenty-one components were characterized in the oil layer. The main constituents were 4aα,7α,7aα-nepetalactone (92.6%), linalyl acetate (2.6%), piperitone (0.80%), germacrene D (0.73%), 4aβ ,7α,7aβ-nepetalactone (0.64%) and β-caryophyllene (0.58%).