7SL RNA from <i>Schizosaccharomyces pombe</i>
 is encoded by a single copy essential gene

Ribes, Véronique; Dehoux, Pierre; Tollervey, David

doi:10.1002/j.1460-2075.1988.tb02804.x

Cited by 68 publications

(37 citation statements)

References 26 publications

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“…cerevisiae and yet 7SL-like molecules appear to be ubiquitous in other organisms (6,41,43), and (iii) that 7SL RNAs are RNA polymerase III transcripts (57) just as scRl appears to have signals for polymerase III transcription, we considered it worthwhile to examine the structure of scRl with reference to the known structures of 7SL RNAs.…”

Section: Discussionmentioning

confidence: 99%

The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth.

1989

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The most abundant RNA visible between 5.8S and 18S rRNA on an ethidium bromide-stained gel of total Saccharomyces cerevisiae RNA has an apparent size of about 600 nucleotides. By purifying the band and using it as a probe to screen a genomic library, we isolated and sequenced the unique gene for this RNA. The transcribed sequence, determined to be 519 nucleotides long, contains elements typical of RNA polymerase III transcription. The RNA is predominantly cytoplasmic, so we called it small cytoplasmic RNA 1 (scRl). ScRl is neither 3'-polyadenylated nor 5'-trimethylguanosine capped. We constructed a null mutation of the gene by deleting 252 base pairs from the transcribed region. Haploid strains carrying the scrl-A lesion grew very slowly, segregated cytoplasmic petites ([rho-]) at high frequency, and showed signs of aberrant cell division.A secondary structure model for scRl shows some of the conserved features of the signal recognition particle 7SL RNAs.The types of RNA in living organisms vary greatly from the most abundant classes, directly involved in translation, to a variety of less abundant classes of diverse or unknown function, loosely termed "small RNAs," of which an increasing number are being shown to be involved in aspects of DNA replication or precursor RNA (pre-RNA) processing (14).In eucaryotes, most of the better-characterized small RNAs are located in the nucleus, where they exist as ribonucleoprotein particles (RNPs). Of these, most of the abundant U small nuclear RNPs are involved in pre-mRNA splicing and 3'-end formation (5, 32, 49); nucleolar U3 small nuclear RNP is associated with pre-rRNA, but its function remains obscure (25,27,40); RNase P matures pre-tRNA (1); telomerase is involved in telomere replication (21); and the role of 7SK RNA is unknown (36, 37).In the cytoplasm, the Ro RNPs contain RNA polymerase 111-transcribed "Y" small RNAs; their functions are unknown, and they are defined as a class of autoimmune antigens (la, 24, 28, 42, 55). Prosomes or cylinder particles appear to be heterogeneous in composition, are distributed throughout both nuclei and cytoplasm, and are even less well understood (29). Nuclear-encoded RNAs are required for the functions of the mitochondrial RNPs (MRP), which processes RNA primers of mitochondrial DNA (mtDNA) synthesis (8), and mtDNA primase, which appears to contain cytoplasmic 5.8S rRNA (56).The best-characterized small cytoplasmic RNA is the 7SL RNA component of the signal recognition particle, whose activity is defined in vitro as mediating the translocation of membrane and secretory proteins into the endoplasmic reticulum (53).In this article we report the characterization of an abundant cytoplasmic RNA of Saccharomyces cerev'isiae and of * Corresponding author.

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Section: Discussionmentioning

confidence: 99%

The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth.

1989

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“…The 7SL RNA gene of yeast appears to have an internal tRNA-like promoter (13). The plant 7SL genes may have evolved from these type 2 promoter-containing genes, perhaps explaining the internal B-box homology in the coding region of the Arabidopsis 7SL genes.…”

Section: -Gsmentioning

confidence: 99%

An upstream U-snRNA gene-like promoter is required for transcription of theArabidopsis thaliana7SL RNA gene

Heard

Filipowicz

Marques³

et al. 1995

Nucl Acids Res

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The genes transcribed by RNA polymerase (pol)111 can be placed into four distinct groups based on the nature and position of their promoter elements. In the higher eukaryotes equivalent genes usually belong to the same sub-type of pol Hi promoters and there are few examples of genes which have changed promoter type during evolution. In this work we demonstrate that the promoter of the Arabidopsis thaliana 7SL RNA gene is located upstream of the coding region and is identical to the promoters of pol III-specific plant U-small nuclear RNA (U-snRNA) genes. Sequence analysis of two different 7SL genes from A.thaliana revealed that both genes contain two sequence elements in their 5' flanking regions identical in sequence and position to the highly conserved USE and TATA elements of the pol III-transcribed plant U-snRNA genes. Mutational analysis of these elements in the At7SL-2 gene indicates that the USE and TATA elements are both necessary and account for >90°% of the transcriptional activity of this gene in transfected plant protoplasts. Within the coding region of both genes there is a sequence element which is a 10/11 nt match to the consensus B-box element of tRNA genes, however, this element is not important for gene activity. These findings distinguish the plant genes from the human 7SL gene, which has both intemal and upstream promoter elements and its upstream elements are different from those found in the human U-snRNA genes.

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“…Mammalian SRP is an 11S particle composed of a 300-nucleotide RNA (7SL) and six polypeptides (28,29). Previous work in several laboratories led to the discovery of a ribonucleoprotein in the fission yeast Schizosaccharomyces pombe that is similar in size to mammalian SRP and contains a 254-nucleotide homolog of human SRP RNA (3,16,18). Disruption of the srp7 gene demonstrated that this RNA is essential for viability in S. pombe (3,18).…”

mentioning

confidence: 99%

“…Previous work in several laboratories led to the discovery of a ribonucleoprotein in the fission yeast Schizosaccharomyces pombe that is similar in size to mammalian SRP and contains a 254-nucleotide homolog of human SRP RNA (3,16,18). Disruption of the srp7 gene demonstrated that this RNA is essential for viability in S. pombe (3,18). The fission yeast homolog of mammalian SRP54 protein (SRP54p) (6) is also required for vegetative growth (1).…”

mentioning

confidence: 99%

Identification of RNA sequences and structural elements required for assembly of fission yeast SRP54 protein with signal recognition particle RNA.

Selinger¹,

Brennwald²,

Liao³

et al. 1993

Mol. Cell. Biol.

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Signal recognition particle (SRP) is a ribonucleoprotein composed of six polypeptides and a single RNA molecule. SRP RNA can be divided into four structural domains, the last of which is the most highly conserved and, in Schizosaccharomycespombe, is the primary location to which deleterious mutations map. The ability of mammalian SRP54 protein (SRP54p) to bind Escherichia coli 4.5S RNA, a homolog of SRP RNA which contains only domain IV, suggested that SRP54p might interact directly with this region. To determine whether domain IV is critical for SRP54p binding in fission yeast cells, we used a native immunoprecipitation-RNA sequencing assay to test 13 mutant SRP RNAs for the ability to associate with the protein in vivo. The G156A mutation, which alters the 5' residue of the noncanonical first base pair of the domain IV terminal helix and confers a mild conditional growth defect, reduces assembly of the RNA with SRP54p. Mutating either of the two evolutionarily invariant residues in the bulged region 5' to G156 is more deleterious to growth and virtually abolishes SRP54p binding. We conclude that the conservation of nucleotides 154 to 156 is likely to be a consequence of their role as a sequence-specific recognition element for the SRP54 protein. We also tested a series of mutants with nucleotide substitutions in the conserved tetranucleotide loop and adjoining stem of domain IV. Although tetraloop mutations are deleterious to growth, they have little effect on SRP54p binding. Mutations which disrupt the base pair flanking the tetraloop result in conditional growth defects and significantly reduce association with SRP54p. Disruption of the other two base pairs in the short stem adjacent to the tetranucleotide loop has similar but less dramatic effects on SRP54p binding. These data provide the first evidence that both sequence-specific contacts and the structural integrity of domain IV of SRP RNA are important for assembly with SRP54p.

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7SL RNA from Schizosaccharomyces pombe is encoded by a single copy essential gene

Cited by 68 publications

References 26 publications

The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth.

The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth.

An upstream U-snRNA gene-like promoter is required for transcription of theArabidopsis thaliana7SL RNA gene

Identification of RNA sequences and structural elements required for assembly of fission yeast SRP54 protein with signal recognition particle RNA.

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