7X multiplexed, optofluidic detection of nucleic acids for antibiotic-resistance bacterial screening

Meena, Gopikrishnan G.; Wall, Thomas A.; Stott, Matthew A.; Brown, O.; Robison, Richard A.; Hawkins, Aaron R.; Schmidt, Holger

doi:10.1364/oe.402311

Cited by 14 publications

(8 citation statements)

References 35 publications

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“…To further improve multiplexing capabilities, Meena et al. (2020) designed a multi‐mode interference (MMI) waveguides optofluidic platform (Figure 7) to fabricate a seven‐plex antibiotic‐resistance bacterial screening method . The MMI waveguides can support multiple modes propagating with different propagation constants, which interfered with each other to produce distinguished spots at specific distances.…”

Section: Strategies and Applications Of Mobas In Food Safety Analysismentioning

confidence: 99%

Multiplex optical bioassays for food safety analysis: Toward on‐site detection

Guan

Jin

et al. 2022

Comp Rev Food Sci Food Safe

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Food safety analysis plays a significant role in controlling food contamination and supervision. In recent years, multiplex optical bioassays (MOBAs) have been widely applied to analyze multiple hazards due to their efficiency and low cost. However, due to the challenges such as multiplexing capacity, poor sensitivity, and bulky instrumentation, the further application of traditional MOBAs in food screening has been limited. In this review, effective strategies regarding food safety MOBAs are summarized, such as spatial‐resolution modes performed in multi‐T lines/dots strips or arrays of strip/microplate/microfluidic chip/SPR chip and signal‐resolution modes employing distinguishable colorimetric/luminescence/fluorescence/surface plasma resonance/surface‐enhanced Raman spectrum as signal tags. Following this, new trends on how to design engineered sensor architecture and exploit distinguishable signal reporters, how to improve both multiplexing capacity and sensitivity, and how to integrate these formats into smartphones so as to be mobile are summarized systematically. Typically, in the case of enhancing multiplexing capacity and detection throughput, microfluidic array chips with multichannel architecture would be a favorable approach to overcome the spatial and physical limitations of immunochromatographic assay (ICA) test strips. Moreover, noble metal nanoparticles and single‐excitation, multiple‐emission luminescence nanomaterials hold great potential in developing ultrasensitive MOBAs. Finally, the exploitation of innovative multiplexing strategy hybridized with powerful and widely available smartphones opens new perspectives to MOBAs. In future, the MOBAs should be more sensitive, have higher multiplexing capacity, and easier instrumentation.

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Section: Strategies and Applications Of Mobas In Food Safety Analysismentioning

confidence: 99%

Multiplex optical bioassays for food safety analysis: Toward on‐site detection

Guan

Jin

et al. 2022

Comp Rev Food Sci Food Safe

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“…The markers fluoresce in this light, and the fluorescence can be detected and measured by a photodetector as a signal for target presence. Targets detectable by this biosensor platform include antigens, cancer biomarkers, liposomes, nucleic acids, proteins, ribosomes, and virions [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…Notably, this biosensor platform has been used to detect the H1N1 virus [7], various antibiotic-resistant bacterial genes [5], and the recent SARS-CoV-2 virus [1]. Meena et al demonstrated the detection of E. coli, E. aerogenes, K. pneumonia, KPC, and VIM with accuracies of 91.6%, 90.2%, 85%, 90%, and 90%, respectively; their tests yielded an average signal-to-noise ratio (SNR) of 39.…”

Section: Introductionmentioning

confidence: 99%

“…Meena et al demonstrated the detection of E. coli, E. aerogenes, K. pneumonia, KPC, and VIM with accuracies of 91.6%, 90.2%, 85%, 90%, and 90%, respectively; their tests yielded an average signal-to-noise ratio (SNR) of 39. Furthermore, they demonstrated the simultaneous detection of multiple targets [5]. Stambaugh et al demonstrated biosensing tests in which influenza A antigens were detected at a rate of 5.7 events per second, and SARS-CoV-2 antigens were detected at a rate of 2.6 events per second; these events were observed with an SNR of 80 [1].…”

Section: Introductionmentioning

confidence: 99%

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Performance Comparison of Flow-Through Optofluidic Biosensor Designs

et al. 2021

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Optofluidic flow-through biosensors are being developed for single particle detection, particularly as a tool for pathogen diagnosis. The sensitivity of the biosensor chip depends on design parameters, illumination format (side vs. top), and flow configuration (parabolic, two- and three-dimensional hydrodynamic focused (2DHF and 3DHF)). We study the signal differences between various combinations of these design aspects. Our model is validated against a sample of physical devices. We find that side-illumination with 3DHF produces the strongest and consistent signal, but parabolic flow devices process a sample volume more quickly. Practical matters of optical alignment are also discussed, which may affect design choice.

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“…Target multiplexing was implemented by exciting different targets with spectrally and/or spatially varying multispot light patterns generated by multimode interference (MMI) waveguides (29). When these were intersected with single or multiple analyte waveguide channels, the target fluorescence signal reflects the different spatial excitation patterns and up to 7× multiplexing has been demonstrated (30)(31)(32)(33)(34)(35). Critically, the flow-based detection on the waveguide chip is target agnostic and has enabled detection of a wide variety of biomolecules including liposomes, virions, nucleic acids, ribosomes, and proteins.…”

mentioning

confidence: 99%

Optofluidic multiplex detection of single SARS-CoV-2 and influenza A antigens using a novel bright fluorescent probe assay

Stambaugh

Parks

Stott

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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The urgency for the development of a sensitive, specific, and rapid point-of-care diagnostic test has deepened during the ongoing COVID-19 pandemic. Here, we introduce an ultrasensitive chip-based antigen test with single protein biomarker sensitivity for the differentiated detection of both severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A antigens in nasopharyngeal swab samples at diagnostically relevant concentrations. The single-antigen assay is enabled by synthesizing a brightly fluorescent reporter probe, which is incorporated into a bead-based solid-phase extraction assay centered on an antibody sandwich protocol for the capture of target antigens. After optimization of the probe release for detection using ultraviolet light, the full assay is validated with both SARS-CoV-2 and influenza A antigens from clinical nasopharyngeal swab samples (PCR-negative spiked with target antigens). Spectrally multiplexed detection of both targets is implemented by multispot excitation on a multimode interference waveguide platform, and detection at 30 ng/mL with single-antigen sensitivity is reported.

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7X multiplexed, optofluidic detection of nucleic acids for antibiotic-resistance bacterial screening

Cited by 14 publications

References 35 publications

Multiplex optical bioassays for food safety analysis: Toward on‐site detection

Multiplex optical bioassays for food safety analysis: Toward on‐site detection

Performance Comparison of Flow-Through Optofluidic Biosensor Designs

Optofluidic multiplex detection of single SARS-CoV-2 and influenza A antigens using a novel bright fluorescent probe assay

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