7β-hydroxycholesterol-induced cell death, oxidative stress, and fatty acid metabolism dysfunctions attenuated with sea urchin egg oil

Zarrouk, Amira; Salem, Yosra Ben; Hafsa, Jawhar; Sghaier, Randa; Charfeddine, Bassem; Limem, Khalifa; Hammami, Mohamed; Majdoub, Hatem

doi:10.1016/j.biochi.2018.06.027

Cited by 16 publications

(13 citation statements)

References 52 publications

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“…Of note, it has been demonstrated that an excess of VLCSFA can induce cell death through proapoptotic pathway, which is related to mitochondrial dysfunction and oxidative stress [96]. In accordance with previous studies, the amounts of monounsaturated fatty acid (MUFAs) and polyunsaturated fatty acid (PUFAs) were also reduced in 158N cells exposed to 7b-OHC [77]. As unsaturated fatty acids (MUFA and PUFA) are required for neuronal cell functions and neuronal membrane fluidity [97,98], this decrease could favour neurodegeneration [98,99].…”

Section: Discussionsupporting

confidence: 85%

“…Our data demonstrate that 7b-OHC-induced side effects are associated with disequilibrium of redox status shown by an enhancement of antioxidant enzyme activities (GP X , SOD, CAT) as well as activation of lipid and protein oxidation [77]. The increase of antioxidant activities under 7b-OHC treatment could be explained by a cell reaction to protect the cells against increased levels of O 2 À and H 2 O 2 .…”

Section: Discussionmentioning

confidence: 63%

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Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes

Sghaier

Zarrouk

Nury

et al. 2019

Free Radical Research

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Lizard (2019) Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes, Free Radical Research, 53:5, 535-561, ABSTRACT Mitochondrial dysfunction and oxidative stress are involved in neurodegenerative diseases associated with an enhancement of lipid peroxidation products such as 7b-hydroxycholesterol (7b-OHC). It is, therefore, important to study the ability of 7b-OHC to trigger mitochondrial defects, oxidative stress, metabolic dysfunctions and cell death, which are hallmarks of neurodegeneration, and to identify cytoprotective molecules. The effects of biotin were evaluated on 158N murine oligodendrocytes, which are myelin synthesizing cells, exposed to 7b-OHC (50 mM) with or without biotin (10 and 100 nM) or a-tocopherol (positive control of cytoprotection). The effects of biotin on 7b-OHC activities were determined using different criteria: cell adhesion; plasma membrane integrity; redox status. The impact on mitochondria was characterized by the measurement of transmembrane mitochondrial potential (DWm), reactive oxygen species (ROS) overproduction, mitochondrial mass, quantification of cardiolipins and organic acids. Sterols and fatty acids were also quantified. Cell death (apoptosis, autophagy) was characterized by the enumeration of apoptotic cells, caspase-3 activation, identification of autophagic vesicles, and activation of LC3-I into LC3-II. Biotin attenuates 7b-OHC-induced cytotoxicity: loss of cell adhesion was reduced; antioxidant activities were normalized. ROS overproduction, protein and lipid oxidation products were decreased. Biotin partially restores mitochondrial functions: attenuation of the loss of DWm; reduced levels of mitochondrial O 2 À overproduction; normalization of cardiolipins and organic acid levels. Biotin also normalizes cholesterol and fatty acid synthesis, and prevents apoptosis and autophagy (oxiapoptophagy). Our data support that biotin, which prevents oligodendrocytes damages, could be useful in the treatment of neurodegeneration and demyelination. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 63%

Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes

Sghaier

Zarrouk

Nury

et al. 2019

Free Radical Research

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“…In 158N cells incubated with 7KC or 7β-OHC, the cytoprotective effect of dimethylfumarate (DMF; Tecfidera) and biotin (vitamin B8), which are used for the treatment of MS, have been shown [120,121]. Natural products including argan oil, milk thistle seed oil, sea urchin egg oil, Carpobrotus edulis ethanol-water extract also strongly attenuate 7KC and/or 7β-OHC-induced cytotoxicity [121][122][123][124][125].…”

Section: -In Vitro Models Applied To the Study Of Neurodegenerative Diseases And The Identification Of Cytoprotective Compounds In This Fmentioning

confidence: 99%

7-Ketocholesterol and 7β-hydroxycholesterol: In vitro and animal models used to characterize their activities and to identify molecules preventing their toxicity

Véjux

Abed-Vieillard

Hajji

et al. 2020

Biochemical Pharmacology

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.Distributed under a Creative Commons Attribution -NonCommercial -NoDerivatives| 4.0International License

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“…Based on this hypothesis, mouse 158N oligodendrocytes were treated with 7β-hydroxycholesterol. This induces cell death associated with oxidative stress (alterations in superoxide dismutase and glutathione peroxidase activities, increased lipid peroxidation and protein carbonylation) [119]. Sea urchin egg oil attenuates the cytotoxicity induced by 7β-hydroxycholesterol.…”

Section: Cytoprotective Effects Of Animal Oilsmentioning

confidence: 99%

“…Sea urchin egg oil attenuates the cytotoxicity induced by 7β-hydroxycholesterol. This result led to the hypothesis that this oil could be useful in the prevention of neurodegenerative diseases and, therefore, possibly Alzheimer's and Parkinson's disease [119].…”

Section: Cytoprotective Effects Of Animal Oilsmentioning

confidence: 99%

Lipids Nutrients in Parkinson and Alzheimer’s Diseases: Cell Death and Cytoprotection

Nury

Lizard

Véjux

2020

IJMS

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Neurodegenerative diseases, particularly Parkinson’s and Alzheimer’s, have common features: protein accumulation, cell death with mitochondrial involvement and oxidative stress. Patients are treated to cure the symptoms, but the treatments do not target the causes; so, the disease is not stopped. It is interesting to look at the side of nutrition which could help prevent the first signs of the disease or slow its progression in addition to existing therapeutic strategies. Lipids, whether in the form of vegetable or animal oils or in the form of fatty acids, could be incorporated into diets with the aim of preventing neurodegenerative diseases. These different lipids can inhibit the cytotoxicity induced during the pathology, whether at the level of mitochondria, oxidative stress or apoptosis and inflammation. The conclusions of the various studies cited are oriented towards the preventive use of oils or fatty acids. The future of these lipids that can be used in therapy/prevention will undoubtedly involve a better delivery to the body and to the brain by utilizing lipid encapsulation.

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7β-hydroxycholesterol-induced cell death, oxidative stress, and fatty acid metabolism dysfunctions attenuated with sea urchin egg oil

Cited by 16 publications

References 52 publications

Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes

Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes

7-Ketocholesterol and 7β-hydroxycholesterol: In vitro and animal models used to characterize their activities and to identify molecules preventing their toxicity

Lipids Nutrients in Parkinson and Alzheimer’s Diseases: Cell Death and Cytoprotection

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