8-[3H]-hydroxy-2-(di-n-propylamino)tetralin binding sites in blood lymphocytes of rats and the modulation by mitogens and immobilization

Sempere, Telésfora; Cedeño, Nemesio; Urbina, Mary; Lima, Lucimey

doi:10.1016/s0165-5728(03)00091-2

Cited by 12 publications

(13 citation statements)

References 66 publications

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“…Con A (0.5 mg/ml), was prepared in a phosphate-buffered saline (PBS; all measurements in m M : 137.9 of NaCl, 0.9 of KCl, 1.15 of KH 2 PO 4 , 8.06 of Na 2 HPO 4 ; pH 7.4) and i.p. injections were done on 2 consecutive days, 500 μg/kg (200-300 μl), between 08.00 and 09.30 h [3]. The rats were physically restricted in a different room in individual ventilated Plexiglass boxes, 16 × 7 × 6 cm, adjustable for length to 9, 11 or 14 cm, for 5 h per day between 09.00 and 14.00 h for 5 days [4,5].…”

Section: Methodsmentioning

confidence: 99%

“…Membrane integrity membrane was evaluated by exclusion of Trypan blue (>96%). According to previous results from the laboratory more blood was obtained by cardiac puncture and each measurement corresponded to 1 rat [3]. …”

Section: Methodsmentioning

confidence: 99%

“…Con A (2.5 μg/ml) [3,24] was added to some of the wells. Aliquots of cells were separated before plating for immunochemical labeling.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In addition, serotonin (5-HT) 1A receptors in lymphocytes are modified by stress protocols, such as physical restraint, or by mitogen activation [3,4] and reserpine treatment [4]. For instance concanavalin A (Con A) increases 5-HT 1A receptors in lymphocytes [3] and affects the effect of fluoxetine on proliferation [4]. Reserpine, acting on the sympathetic system as an inhibitor of vesicular transport of monoamines, constitutes a documented pharmacological stress [5] and alters the 5-HT transporter in lymphocytes [6].…”

Section: Introductionmentioning

confidence: 99%

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5-HT<sub>7</sub> Receptors and Tryptophan Hydroxylase in Lymphocytes of Rats: Mitogen Activation, Physical Restraint or Treatment with Reserpine

Urbina

Arroyo²,

Lima³

2014

Neuroimmunomodulation

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Objectives: Serotonin (5-HT)₇ receptors in lymphocytes play a relevant role as modulators of T cell functions and might be modified by stress protocols. The aims of this work were to evaluate: (i) the presence of 5-HT₇ receptors in specific lymphocyte populations, (ii) the probable modifications of them by inflammatory stress with mitogen and (iii) the effects of physical and pharmacological stress. Methods: Blood lymphocytes were isolated by density gradients and differential adhesion to plastic. Concanavalin A (Con A) was systemically administered (500 μg/kg) or added to lymphocyte cultures (2.5 μg/ml, final volume 200 μl). Physical restraint was performed in Plexiglass boxes for 5 h per day for 5 days. Reserpine administration was 2.5 mg/kg for 3 days. Immunocytochemical labeling of CD4+, CD8+ and 5-HT₇ receptors, and also tryptophan hydroxylase cells was performed. mRNA of 5-HT₇ receptors was evaluated by RT-PCR. Controls were included for each protocol. Results: Con A treatment or culture exposure increased the number of lymphocytes expressing 5-HT₇ receptors or tryptophan hydroxylase, as compared to absence of the mitogen. Receptors were present in 12-16% of total rat lymphocytes, in ∼10% of CD4+ and in ∼5% of CD8+ cells from control rats. CD4+ decreased, and CD8+ and 5-HT₇ cells increased after physical restraint. Reserpine treatment elevated CD8+ and 5-HT₇ cells. Con A and physical restraint, but not reserpine treatment, significantly augmented 5-HT₇ receptor mRNA in lymphocytes. Conclusions: Rat lymphocytes, expressing tryptophan hydroxylase, could synthesize 5-HT, functioning as a direct autocrine modulator. The modifications of CD4+, CD8+ and 5-HT₇ receptors in lymphocytes by three stress protocols could have an impact on immune responses. In addition, the differential distribution of 5-HT₇ receptors indicates potential specific physiopathological roles.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Con A (2.5 μg/ml) [3,24] was added to some of the wells. Aliquots of cells were separated before plating for immunochemical labeling.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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5-HT<sub>7</sub> Receptors and Tryptophan Hydroxylase in Lymphocytes of Rats: Mitogen Activation, Physical Restraint or Treatment with Reserpine

Urbina

Arroyo²,

Lima³

2014

Neuroimmunomodulation

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Role of 5-HT2 and 5-HT7 Serotonin Receptors, and Protein Kinases C and A on Taurine Transport in Lymphocytes of Rats Treated with Fluoxetine

Colmenares-Aguilar

Lima

2017

Advances in Experimental Medicine and Biology

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Fluoxetine, an antidepressant and selective serotonin reuptake inhibitor, modulates immune cells in vitro. The present study investigates the influence of pharmacological agents which acts as agonist and antagonist of serotonin receptors ex vivo over taurine transport in lymphocytes of rats treated with fluoxetine by one week. The treatment with fluoxetine increase taurine transport and the incubation with the agonist of 5-HT receptor, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) counteract this effect, and ketanserin provoked no change in fluoxetine effect. While the agonist of 5-HT receptor, 4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphth alenyl)-1-piperazinehexanamide hydrochloride (LP44) had no significant effects, however the differences between Control and Fluoxetine groups were not observed, the antagonist (R)-3-[2-[2-(4-methylpiperidin-1-yl)ethyl]pyrrolidine-1-sulfonyl]phenol hydrochloride (SB269970) had no differences. Preincubation of cells with the diacylglycerol analogue, 1-oleoyl-2-acetyl-sn-glycerol (OAG) caused inhibition of fluoxetine treatment effect but this not occurred in presence of the PKC inhibitor, 1-O-hexadecyl-2-O-methyl-rac-glycerol (AMG-C). Forskolin counteracted the effect of fluoxetine on taurine transport, since at the concentrations used, the rate of taurine transport in Fluoxetine group, returned to Control rate. No significant differences were observed with the PKA inhibitor. Although it is not possible to attribute a definitive role of 5-HT receptors in fluoxetine effect on taurine transport, its signaling might affect the function of it. Participation of PKC and PKA have an apparently relevant role in lymphocyte taurine transport.

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Differential cAMP levels and serotonin effects in blood peripheral mononuclear cells and lymphocytes from major depression patients

2004

International Immunopharmacology

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8-[3H]-hydroxy-2-(di-n-propylamino)tetralin binding sites in blood lymphocytes of rats and the modulation by mitogens and immobilization

Cited by 12 publications

References 66 publications

5-HT<sub>7</sub> Receptors and Tryptophan Hydroxylase in Lymphocytes of Rats: Mitogen Activation, Physical Restraint or Treatment with Reserpine

5-HT<sub>7</sub> Receptors and Tryptophan Hydroxylase in Lymphocytes of Rats: Mitogen Activation, Physical Restraint or Treatment with Reserpine

Role of 5-HT2 and 5-HT7 Serotonin Receptors, and Protein Kinases C and A on Taurine Transport in Lymphocytes of Rats Treated with Fluoxetine

Differential cAMP levels and serotonin effects in blood peripheral mononuclear cells and lymphocytes from major depression patients

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