8 Engineering of cyanobacteria for increased hydrogen production

Lindblad, Peter; Khanna, Namita

doi:10.1515/9783110336733.173

Cited by 1 publication

(2 citation statements)

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“…Different strategies have been used to improve the hydrogen production capacity of photosynthetic organisms [ 19 , 20 , 32 ]. The introduction of non-native pathways has been explored either by expression of a catalytically efficient [FeFe] hydrogenase or a comparatively more oxygen and thermo-tolerant [NiFe] homolog of A. macleodii HynSL hydrogenase, as described in the section above [ 88 , 89 , 90 , 91 ].…”

Section: Key Advances In Cyanobacterial Researchmentioning

confidence: 99%

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Cyanobacterial Hydrogenases and Hydrogen Metabolism Revisited: Recent Progress and Future Prospects

Khanna

Lindblad

2015

IJMS

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Cyanobacteria have garnered interest as potential cell factories for hydrogen production. In conjunction with photosynthesis, these organisms can utilize inexpensive inorganic substrates and solar energy for simultaneous biosynthesis and hydrogen evolution. However, the hydrogen yield associated with these organisms remains far too low to compete with the existing chemical processes. Our limited understanding of the cellular hydrogen production pathway is a primary setback in the potential scale-up of this process. In this regard, the present review discusses the recent insight around ferredoxin/flavodoxin as the likely electron donor to the bidirectional Hox hydrogenase instead of the generally accepted NAD(P)H. This may have far reaching implications in powering solar driven hydrogen production. However, it is evident that a successful hydrogen-producing candidate would likely integrate enzymatic traits from different species. Engineering the [NiFe] hydrogenases for optimal catalytic efficiency or expression of a high turnover [FeFe] hydrogenase in these photo-autotrophs may facilitate the development of strains to reach target levels of biohydrogen production in cyanobacteria. The fundamental advancements achieved in these fields are also summarized in this review.

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Section: Key Advances In Cyanobacterial Researchmentioning

confidence: 99%

“…At present, cyanobacteria and green algae are considered optimal candidates for hydrogen evolution, being the only known organisms that can both express functional hydrogenase and perform oxygenic photosynthesis [ 4 , 19 , 20 ]. The two organisms possess two distinct classes of hydrogenase; [NiFe] and [FeFe] hydrogenase respectively.…”

Section: Introductionmentioning

confidence: 99%