8-Iso-Prostaglandin F_2α as a Useful Clinical Biomarker of Oxidative Stress in ESRD Patients

Abstract

Background/Aims: Chronic renal failure is associated with elevated indices of oxidative stress. We tested the hypothesis that the in vivo formation of the F₂-isoprostane (8-iso-prostaglandin PGF_2α), a bioactive product of arachidonic acid peroxidation, is enhanced in end-stage renal disease (ESRD) patients receiving hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD). Methods: Plasma samples were obtained from 35 HD patients, 30 CAPD patients and 30 age- and sex-matched healthy subjects for measurement of immunoreactive 8-iso-PGF_2α. Results: Plasma 8-iso-PGF_2α levels were significantly higher (p < 0.001) in HD and CAPD patients (346.3 ± 132.4 pg/ml; range 49.8–870) than in age-matched control subjects (150.9 ± 61.6 pg/ml; range 33.5–235). In addition, we also found that 8-iso-PGF_2α concentration was significantly (p = 0.007) higher in HD patients (389.8 ± 148.3 pg/ml) than in CAPD patients (254.3 ± 76.6 pg/ml). Plasma 8-iso-PGF_2α concentration was linearly correlated with serum haptoglobin, C-reactive protein (CRP) and plasma MDA (r = 0.58, p = 0.003; r = 0.29, p < 0.05 and r = 0.38, p < 0.05 respectively). On the other hand, plasma 8-iso-PGF_2α levels were inversely associated with serum albumin and total cholesterol (r = –0.31 and r = –0.28, respectively; p < 0.05). Conclusions: We conclude that ESRD on both HD and CAPD is associated with increased formation of F₂-isoprostanes, a correlate of enhanced lipid peroxidation. We also found that plasma 8-iso-PGF_2α was casually related to some acute phase reactant proteins such as serum CRP, albumin and haptoglobin. This may provide an important biochemical link between lipid peroxidation, inflammation and accelerated atherosclerosis in the uremic milieu.

Changes in the Concentrations of Mediators of Inflammation and Oxidative Stress in Exhaled Breath Condensate During Liver Transplantation and Their Relations With Postoperative ARDS

“…11 Thus, it has become increasingly important to develop an ability to predict and diagnose ARDS as early as possible. 12 In this study, we found that levels of inflammatory factors (TNF-␣ and IL-8) 5 and mediators of oxidative stress (MDA, H 2 O 2 , NO, and 8-iso-prostaglandin F 2␣ ) 13,14 in both EBC and serum were significantly elevated in subjects with ARDS but that concentrations of IL-10 (an anti-inflammatory cytokine) and SOD (an antioxidant) in EBC and serum both decreased in subjects with ARDS. We also found that all these mediator increases correlate with the incidence of ARDS following OLT and some other poor prognoses.…”

Oxidative Stress and IgG Antibody Modify Periodontitis-CRP Association

“…In cross-sectional studies, increased serum concentrations of 8-isoprostane have been correlated with increased levels of serum CRP in patients with end-stage renal disease (Lim et al 2002), stroke patients (Sánchez-Moreno et al 2004), and a healthy adult population (Dohi et al 2007). Our findings indicate physiologic increases in serum 8-isoprostane concentrations relate to significant increases in serum CRP levels in a representative adult population.…”

Profound Mishandling of Protein Glycation Degradation Products in Uremia and Dialysis

“…The concentration of FL residues may be decreased in CRF and HD patients by increased oxidative stress with conversion of FL to CML residues (15). High levels of glycoxidation adducts (pentosidine and CML) in HD may reflect increased oxidative stress, relative to PD (29). The concentrations of MG-H1, MetSO, and 3-NT residues were normalized in HD patients after dialysis.…”