807 Evaluation of biocompatibility of alloplastic materials – development of a tissue culture in vitro test system

Introduction: Mesh-related complications especially after vaginal implantation have raised awareness lately because of severe adverse reactions and legal aspects. About 20% of patients suffer from complications after mesh insertion in the anterior vaginal wall. Autologous plasma coating of meshes prior to implantation has shown potential to improve the biocompatibility of meshes in vivo and in vitro. This inno-

show abstract

“…Those findings were shown to be consistent under both in vivo and in vitro conditions [15][16][17][18].…”

Section: Introductionsupporting

confidence: 63%

“…It will not be possible to get this information via examination features, etc., either for the patient or for the physician involved in the follow-up. In cases of necessary explantation immunohistochemistry analyses and in vitro biocompatibility tests of the explanted mesh will be performed [15].…”

Section: Documentation and Data Handlingmentioning

confidence: 99%

Protocol for a Randomized Phase II Trial for Mesh Optimization by Autologous Plasma Coating in Prolapse Repair: IDEAL Stage 3

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Table 2 Surgical and perioperative characteristics. studies we found beneficial characteristics of PVDF-polymer with reduced inflammatory reaction and scarring at the implantation site [9,11]. Several studies on PVDF mesh application for surgical hernia repair are available.…”

Section: Discussionmentioning

confidence: 54%

“…Biocompatibility is an important requirement for the perfect ingrowth of the material. It is determined by the foreign body reaction and inflammation and depends on different parameters like type of polymer, pore size, material weight and others [8,9]. An optimized surgical mesh permits the transmigration and localisation of immune cells and prevents adherence and inflammation if directly exposed to visceral organs, vessels or nerves.…”

Section: Introductionmentioning

confidence: 99%

“…Experimental studies have been conducted first and can be assigned to preclinical stage 0 according to IDEAL-D [13]. For the first time a validated in vitro test system to compare the biocompatibility of different meshes was developed [9]. Series of commonly used meshes were incubated with patient tissue culture (muscle, connective tissue and endothelium) and the adhesion and ingrowth of tissue over time were assessed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transvaginal PVDF-mesh for cystocele repair: A cohort study

Barski

Arndt

Gerullis

et al. 2017

International Journal of Surgery

View full text Add to dashboard Cite

h i g h l i g h t sPVDF-mesh is feasible for transvaginal application for cystocele repair. 15% of patients presented any kind of complications and 8.8% needed a resurgery for SUI or POP during the follow-up. Improvement of quality of life was reported by 87.5% according the PGI-I. The preliminary results support the initiation of prospective trial and registry according to IDEAL. a r t i c l e i n f o Results: 34 women were eligible for a mean follow up of 19 months. The functional outcome improved significantly. One symptomatic vault prolapse (2.9%) and two reoperations for incontinence (5.9%) occurred. Two mesh exposures (5.9%) occurred and were treated conservatively. No other severe complications were registered. 87.5% of treated women felt very much better or much better and would undergo the surgery again. A cohort study including development steps in accordance with the IDEAL system is presented. Conclusions: For the first time we report on effectivity and safety of transvaginal application of PVDFmesh in real-life practice. A prospective long-term evaluation in a registry is justified.

show abstract

Coating with Autologous Plasma Improves Biocompatibility of Mesh GraftsIn Vitro: Development Stage of a Surgical Innovation

Gerullis

Georgas

Eimer

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Purpose. To investigate mesh coating modalities with autologous blood components in a recently developed in vitro test system for biocompatibility assessment of alloplastic materials. Materials and Methods. Seven different mesh types, currently used in various indications, were randomly investigated. Meshes were coated prior to cultivation with autologous peripheral blood mononuclear cells (PBMCs), platelets, and blood plasma. Pretreated meshes were incubated over 6 weeks in a minced tissue assay, representative for fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. Adherence of those tissues on the meshes was microscopically investigated and semiquantitatively assessed using a previously described scoring system. Results. Coating with peripheral blood mononuclear cells did not affect the adherence score, whereas coating with platelets and blood plasma increased the score suggesting improved biocompatibility in vitro. The previous ranking of native meshes remained consistent after coating. Conclusion. Plasma coating of meshes improves their biocompatibility score in a novel in vitro test system.

show abstract

807 Evaluation of biocompatibility of alloplastic materials – development of a tissue culture in vitro test system

Cited by 10 publications

References 0 publications

Protocol for a Randomized Phase II Trial for Mesh Optimization by Autologous Plasma Coating in Prolapse Repair: IDEAL Stage 3

Protocol for a Randomized Phase II Trial for Mesh Optimization by Autologous Plasma Coating in Prolapse Repair: IDEAL Stage 3

Transvaginal PVDF-mesh for cystocele repair: A cohort study

Coating with Autologous Plasma Improves Biocompatibility of Mesh GraftsIn Vitro: Development Stage of a Surgical Innovation

Contact Info

Product

Resources

About