825 Intestinal Inflammation Targets E. Coli NC101 Transcriptome Response and Promotes Development of Colorectal Cancer (CRC)

Muehlbauer, Marcus; Gharaibeh, Raad Z.; Arthur, Janelle C.; Fodor, Anthony A.; Jobin, Christian

doi:10.1016/s0016-5085(13)60520-x

Cited by 3 publications

(2 citation statements)

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“…In addition, it has been reported that genes present in the pks island are significantly induced during development of inflammation and CRC 35. In this study, using mouse models and human CRC biopsy specimens, we show that colibactin-producing E. coli contribute to the emergence of senescent cells, which enhance tumour promotion via growth factor secretion (see online supplementary figure S15).…”

Section: Discussionmentioning

confidence: 55%

Bacterial genotoxin colibactin promotes colon tumour growth by inducing a senescence-associated secretory phenotype

Cougnoux

Dalmasso

Martinez

et al. 2014

Gut

396

371

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Section: Discussionmentioning

confidence: 55%

Bacterial genotoxin colibactin promotes colon tumour growth by inducing a senescence-associated secretory phenotype

Cougnoux

Dalmasso

Martinez

et al. 2014

Gut

396

371

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“…The intestinal microbiota is crucial for regulating susceptibility to pathogens [3], maintaining alkalinity/acidity and oxygen concentration [4,5], and modulating the host immune system [6,7]. Pathogenic bacteria such as Citrobacter rodentium [5], Helicobacter hepaticus [8] and adherent-invasive Escherichia coli (AIEC) NC101 [9], promote intestinal inflammation and induce tumorigenesis. The host, conversely, imposes selective forces on bacterial growth throughout the inflammatory microenvironment.…”

Section: Introductionmentioning

confidence: 99%

A SNP of bacterial blc disturbs gut lysophospholipid homeostasis and induces inflammation through epithelial barrier disruption

et al. 2020

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Background: Alteration of commensal bacterial composition is associated with many inflammatory diseases. However, few studies have pinpointed the specific bacterial genes that may suppress host immune responses against microbes and maintain homeostasis in the host intestine. Methods: High-throughput screening was performed in Caenorhabditis elegans with a single gene knockout Escherichia coli (E. coli) library and identified the immune suppression gene blc. The coding sequences of blc among different kinds of E. coli strains were aligned to identify the single nucleotide polymorphisms (SNPs). Physiological and biochemical experiments were performed in C. elegans and mice to explore the function of the blc variant. Findings: By screening 3983 E. coli mutants, we discovered that 9 bacterial genes, when deleted, activate innate immunity in the host C. elegans. Among these 9 genes, the gene encoding blc showed a distinctive SNP in many clinically pathogenic bacteria. We found that bacteria with this SNP, which converts Blc G84 to Blc E84 , are highly enriched in the faeces of patients with inflammatory bowel disease (IBD). Exposure to Blc E84-encoding bacteria resulted in epithelial barrier disruption and immune activation in both worms and mice. Detailed analysis indicated that infection with Blc E84-encoding bacteria causes a significant decrease in LPE levels in the intestine and subsequently disrupts gut epithelial integrity in mice. Consistently, the levels of LPE in patients with IBD are significantly lower than those in healthy people. Finally, supplementation with LPE, which activates LPA 1 /PLCb/PKC signaling, reversed the defects induced by Blc E84-encoding bacteria. Interpretation: Our results identified a novel bacterial gene, blc, in E. coli that regulates host gut integrity and immunity.

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Microbiome and Diseases: Colorectal Cancer

Iftekhar

Sperlich

Janssen

et al. 2018

The Gut Microbiome in Health and Disease

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825 Intestinal Inflammation Targets E. Coli NC101 Transcriptome Response and Promotes Development of Colorectal Cancer (CRC)

Cited by 3 publications

References 0 publications

Bacterial genotoxin colibactin promotes colon tumour growth by inducing a senescence-associated secretory phenotype

Bacterial genotoxin colibactin promotes colon tumour growth by inducing a senescence-associated secretory phenotype

A SNP of bacterial blc disturbs gut lysophospholipid homeostasis and induces inflammation through epithelial barrier disruption

Microbiome and Diseases: Colorectal Cancer

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