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You, Hong; Yu, Lei

doi:10.1023/a:1009876320336

Cited by 68 publications

(19 citation statements)

References 82 publications

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“…Typically, single cells are mechanically tested using one of four techniques: micropipette aspiration (Guilak et al 2000, Hochmuth 2000, Theret et al 1988), atomic force microscopy (AFM) (Darling et al 2010, Darling et al 2007, Darling et al 2006, Ikai 2009, You and Yu 1999), cytoindentation,(Koay et al 2003, Shin and Athanasiou 1999) or unconfined compression (Leipzig and Athanasiou 2008, Leipzig et al 2006, Ofek et al 2009, Shieh and Athanasiou 2007). Because of the size of the pipette or probe used in micropipette aspiration and atomic force microscopy, these techniques are most useful for measuring the mechanical properties of subcellular components such as portions of the cellular membrane.…”

Section: Introductionmentioning

confidence: 99%

Biomechanics of meniscus cells: regional variation and comparison to articular chondrocytes and ligament cells

Athanasiou

2012

Biomech Model Mechanobiol

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Central to understanding mechanotransduction in the knee meniscus is the characterization of meniscus cell mechanics. In addition to biochemical and geometric differences, the inner and outer regions of the meniscus contain cells that are distinct in morphology and phenotype. This study investigated the regional variation in meniscus cell mechanics in comparison to articular chondrocytes and ligament cells. It was found that the meniscus contains two biomechanically distinct cell populations, with outer meniscus cells being stiffer (1.59 ± 0.19 kPa) than inner meniscus cells (1.07 ± 0.14 kPa). Additionally, it was found that both outer and inner meniscus cell stiffnesses were similar to ligament cells (1.32 ± 0.20 kPa), and articular chondrocytes showed the highest stiffness overall (2.51 ± 0.20 kPa). Comparison of compressibility characteristics of the cells showed similarities between articular chondrocytes and inner meniscus cells, as well as between outer meniscus cells and ligament cells. These results show that cellular biomechanics vary regionally in the knee meniscus, and that meniscus cells are biomechanically similar to ligament cells. The mechanical properties of musculoskeletal cells determined in this study may be useful for the development of mathematical models or the design of experiments studying mechanotransduction in a variety of soft tissues.

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Section: Introductionmentioning

confidence: 99%

Biomechanics of meniscus cells: regional variation and comparison to articular chondrocytes and ligament cells

Athanasiou

2012

Biomech Model Mechanobiol

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“…Atomic force microscopy (AFM) is a useful technique to elucidate surface structure of biological materials at high resolution [8,9]. A major advantage of AFM is that it can be operated under aqueous and physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

“…A major advantage of AFM is that it can be operated under aqueous and physiological conditions. The ability to perform AFM imaging in a physiological environment makes it possible to monitor important biological processes in real time at high resolution [8,9]. Currently, there is a great deal of interest in AFM studies of the interaction between proteins and supported planar lipid membranes (SPLMs), and of the role of lipids in influencing the activity of membrane‐bound and/or associated proteins [10–14].…”

Section: Introductionmentioning

confidence: 99%

Phospholipid membrane restructuring induced by saposin C: a topographic study using atomic force microscopy

You

2001

FEBS Letters

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The enzymatic activity of glucosylceramidase depends on the presence of saposin C (Sap C) and acidic phospholipidcontaining membranes. In order to delineate the mechanism underlying Sap C stimulation of the enzyme activity, it is important to understand how Sap C interacts with phospholipid membranes. We studied the dynamic process of Sap C interaction with planar phospholipid membranes, in real time, using atomic force microscopy (AFM). The phospholipid membrane underwent restructuring upon addition of Sap C. The topographic characteristics of the membrane restructuring include the appearance of patch-like new features, initially emerged at the edge of phospholipid membranes and extended laterally with time. Changes in the image contrast of the phospholipid membrane observed after the Sap C addition indicate that a new phase of lipid^protein structure has formed during membrane restructuring. The process of membrane restructuring is dynamic, commencing shortly after Sap C addition, and continuing throughout the duration of AFM imaging (about 30 min, sometimes over 1 h). This study demonstrated the potential of AFM real-time imaging in studying protein^membrane interactions. ß 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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“…This emerging field of study is being driven by new AFM-based mechanical or rheological measurements of supramolecular biological materials [1,2] and even entire cells [3,4]. Perhaps the prototypical examples of such a material are found in the plethora of viral capsids.…”

Section: Introductionmentioning

confidence: 99%

Nanorheology of viscoelastic shells: Applications to viral capsids

Kuriabova

Levine

2008

Phys. Rev. E

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We study the microrheology of nanoparticle shells [Dinsmore et al. Science 298, 1006(2002] and viral capsids [Ivanovska et al. PNAS 101, 7600 (2004)] by computing the mechanical response function and thermal fluctuation spectrum of a viscoelastic spherical shell that is permeable to the surrounding solvent. We determine analytically the damped dynamics of the shear, bend, and compression modes of the shell coupled to the solvent both inside and outside the sphere in the zero Reynolds number limit. We identify fundamental length and time scales in the system, and compute the thermal correlation function of displacements of antipodal points on the sphere and the mechanical response to pinching forces applied at these points. We describe how such a frequency-dependent antipodal correlation and/or response function, which should be measurable in new AFM-based microrheology experiments, can probe the viscoelasticity of these synthetic and biological shells constructed of nanoparticles.

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Cited by 68 publications

References 82 publications

Biomechanics of meniscus cells: regional variation and comparison to articular chondrocytes and ligament cells

Biomechanics of meniscus cells: regional variation and comparison to articular chondrocytes and ligament cells

Phospholipid membrane restructuring induced by saposin C: a topographic study using atomic force microscopy

Nanorheology of viscoelastic shells: Applications to viral capsids

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