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Feria, Manuel de; Jiménez, Elio; Barbón, Raúl; Capote, Alina; Chávez, Maité; Quiala, Elisa

doi:10.1023/a:1021202305692

Cited by 29 publications

(12 citation statements)

References 20 publications

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“…The numbers of somatic embryos increased in the presence of 80 % DO. However, most of the embryos were at the globular and heart-shaped stages and only 6.6 % were torpedo shaped (De Feria et al 2003). A decrease to 50 % DO increased the number of torpedo-shaped embryos to 20 %.…”

Section: Other Factorsmentioning

confidence: 94%

Somatic Embryogenesis in Coffea spp.

Loyola‐Vargas

Avilez-Montalvo

Aviles-Montalvo

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Coffee is a very important commodity in the world. It is a perennial plant and as a consequence its genetic improvement had been difficult. The biotechnology, in particular somatic embryogenesis (SE) has helped to the propagation of commercial coffee cultivars. Along almost 50 years, different research groups around the world have developed uncountable SE processes to produce an efficient, reproducible, and reliable SE process. Many of the factors that affect the embryogenic response have been studied. It has been demonstrated that the genetic modification of coffee is possible. This transformation can lead to the improvement of varieties with very attractive agronomic traits, such as low caffeine content, resistant to diseases and pests. IntroductionCoffee is one of the two major commodities in the world. The cultivation of coffee gives employment to more than 80 million people, and every year, more than 11 million hectares are cultivated around the world.All coffee species are trees or perennial woody bushes. The coffee plants differ among themselves in different aspects such as morphology, size, and ecological adaptation. Although there are more than 124 species of the Coffea genus, only two are used to produce coffee commercially, Coffea arabica and C. canephora, which accounts for 60 and 40 % of world coffee production, respectively. C. canephora (2n = 2x = 22) is an outcrossing diploid, allogamous, consisting of polymorphic populations of strongly heterozygous individuals and with high caffeine content. C. arabica is an allotetraploid (2n = 4x = 44) derived from a spontaneous

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Section: Other Factorsmentioning

confidence: 94%

Somatic Embryogenesis in Coffea spp.

Loyola‐Vargas

Avilez-Montalvo

Aviles-Montalvo

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

View full text Add to dashboard Cite

show abstract

“…However, most of the embryos were at the globular and heart-shaped stages and only 6.6 % were torpedo shaped (De Feria et al 2003). Catimor 9722.…”

Section: Other Factorsmentioning

confidence: 99%

Somatic Embryogenesis: Fundamental Aspects and Applications

Ochoa-Alejo¹,

Loyola‐Vargas²

2016

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“…In Hordeum vulgare, it was found that the somatic embryogenesis potential of the culture in a 2 L suspension culture bioreactor was significantly reduced compared with the traditional small container [28]. Studies have shown that high oxygen concentrations are conducive to the proliferation of suspension, while low oxygen concentrations are conducive to transformation and differentiation [29]. It shows that the conditions of suspension culture should be appropriately changed according to the purpose of the culture.…”

Section: Discussionmentioning

confidence: 99%

Suspension Culture and Somatic Embryogenesis of Korean Pine

Peng¹,

Gao²,

Wang³

et al. 2022

Phyton

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Korean pine is an important afforestation tree species in Northeast China, which has a high ecological and economic value. Although regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful, it cannot be applied to automation and large-scale production. Therefore, we urgently need a method that can increase the output of somatic embryos (SEs) to meet the needs of large-scale production. We used Korean pine 1-1 and 1-100 cell lines as research materials to evaluate the effects of inoculum-density, culture time, orbiting speed, vessel volume, plant growth regulator (PGR) concentration, and carbon source on the proliferation of embryogenic tissue (ET). The somatic embryogenesis ability of ET cultured in different liquid suspension media was also evaluated. We found that during liquid suspension culture of Korean pine ET, the sedimented cell volume (SCV), fresh weight (FW) and dry weight (DW) were affected by inoculumdensity, culture time, orbiting speed, 2,4-D concentration, 6-BA concentration and carbon source type. Fourty mg ⋅ mL −1 ET were transferred to a 200 mL Erlenmeyer flask containing 20 mL liquid medium, and cultured at 100 rpm/min for 14 days to obtain the maximum proliferation. In addition, we also found that SCV, FW and DW were higher when PGRs were reduced in the liquid suspension medium. The substitution of maltose for sucrose resulted in slow growth of cultures and limited SE yield (13 SEs g −1 FW). Although culture proliferation was high at 50 rpm, SE yield was inhibited by 48% compared with 100 rpm (50 rpm = 33 SEs g −1 FW; 100 rpm/min = 70 SEs g −1 FW). Cultivation in low-concentration PGR(1.15 μM ⋅ L −1 2,4-D, 0.25 μM ⋅ L −1 6-BA) and sucrose liquid medium at 100 rpm/min (80 SEs g −1 FW) could not only promote culture proliferation but also increase SE yield. The determination of the suspension culture scheme of Korean pine ET provides a reference for further expansion to bioreactor culture in the future and lays a foundation for the automation and scale of somatic embryogenesis of Korean pine.

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Cited by 29 publications

References 20 publications

Somatic Embryogenesis in Coffea spp.

Somatic Embryogenesis in Coffea spp.

Somatic Embryogenesis: Fundamental Aspects and Applications

Suspension Culture and Somatic Embryogenesis of Korean Pine

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