[83a] Glycopeptide transpeptidase and d-alanine carboxypeptidase in Escherichia coli

Polymerization by transglycosylation in the biosynthesis of the peptidoglycan of Escherichia coli K 12 and its inhibition by antibiotics

“…Table 2 Effects of antibiotics on the transglycosylation reaction catalysing the formation of peptidoglycan The results obtained with ristocetin and vancomycin were similar to [3] for another system from E. coli. Compound 11837 R.P.…”

“…Since lysozyme digestion yielded mainly this fragment, it was concluded that uncrosslinked peptidoglycan was formed and that the assay was specific for the transglycosylation reaction. Our material thus resembled the soluble peptidoglycan synthesized from the nucleotide precursors under other conditions [3,8,9,16]. The length of the glycan strands and the nature of the reducing ends are now being examined.…”

“…After localization with a 47r Tracerlab scanner the radioactive spots were cut out and converted into 14C02 by combustion in an Oxymat apparatus (Intertechnique, Plaisir, 78, France). r4C02 was trapped in 18 ml solvent containing 7 g biphenyl-4-butyl-PBD[(biphenylyl-4)-2-(tert-butyl-4-phenyl)-5-oxadiazole-1, 3,4] for 100 ml water, 220 ml methanol, 330 ml phenethylamine and 400 ml toluene. Samples were counted by liquid scintillation spectrometry.…”

Enzymes Synthesizing and Hydrolyzing Murein in Escherichia coli

“…UDP-MurNAc-pentapeptide, unlabeled and labeled in the terminal D-alanine (I4C; 29 Ciimol), was given to us by Dr D. Mirelman and was prepared as described by Tzaki et al [9] ; 2-keto-3-deoxyoctonate was kindly supplied by Dr 0. Liideritz.…”

Inhibition by penicillin of the incorporation and cross‐linking of L‐lysine in intact cells of Micrococcus luteus

“…This conclusion has been based on studies both in vitro and in vivo in a limited number of organisms, mainly Escherichia coli [ 1,2], Bacillus megaterium [3], and Staphylococcus aureus [4-61.…”