#85 Association of serum antibodies to the helicobacter pylori caga antigen with precancerous gastric lesions in chinese populations with contrasting gastric cancer rates

Groves, Frank D.; Perez-Perez, Guillermo I.; Zhang, L; You, WC; Lipsitz, S.; Gail, MH; Fraumeni, JosephF.; Blaser, M. J.

doi:10.1016/s1047-2797(02)00373-3

Cited by 8 publications

(10 citation statements)

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“…Most of the Chinese H. pylori strains were cagA-positive (79/82, 96 . 3 %), irrespective of gastroduodenal pathology; this provides further confirmation that cagA is not reliable for discriminating specific gastroduodenal disease-associated H. pylori strains in China (Pan et al, 1997;Groves et al, 2002;Lai et al, 2002). The fact that two of five strains that were not detectable by cagAF and cagAR were verified to be cagA-positive by amplifying fragments that included the cagA 39 variable region showed that the sensitivity of PCR methods for detecting cagA may be crucial for determining cagA status and its variants, especially when H. pylori strains are isolated from different populations or different geographical regions.…”

Section: Discussionmentioning

confidence: 83%

“…Several studies have implicated CagA in the development of duodenal ulcer and gastric adenocarcinoma in most western populations (Covacci et al, 1993;Blaser et al, 1995;Torres et al, 1998;Bach et al, 1999;Miehlke et al, 2000;Abasiyanik et al, 2002;Nomura et al, 2002a, b;Oliveira et al, 2003;Wu et al, 2003), whereas the majority of reports from east Asian countries (Miehlke et al, 1996;Pan et al, 1997;Shimoyama et al, 1997;Maeda et al, 1998;Zheng et al, 2000;Groves et al, 2002;Lai et al, 2002), as well as those from Estonia (Andreson et al, 2002) and Argentina (Catalano et al, 2001), showed a very high prevalence of cagA-positive H. pylori, irrespective of clinical manifestation. Furthermore, it was reported that a PCR primer set that amplified cagA from H. pylori isolated in one country failed to detect cagA in isolates from another country (Miehlke et al, 1996;Pan et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases

Zhou

Zhang

et al. 2004

Journal of Medical Microbiology

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Previous studies have implicated CagA [encoded by cytotoxin-associated gene A (cagA)] in Helicobacter pylori-associated gastroduodenal pathology and distinct subgenotypes of cagA may circulate in different pathological manifestations of cagA-positive H. pylori infection. To investigate cagA genotype and variants in Chinese H. pylori strains and explore their relationship with gastroduodenal diseases, the cagA status of 82 Chinese H. pylori strains was examined and variation in size of the 39 region of cagA in 71 of these strains was analysed by PCR. cagA was detected in 28 (100 %) of 28 strains from peptic ulcer patients, two (100 %) of two strains from gastric cancer patients, 32 (94 . 1 %) of 34 strains from chronic gastritis patients and 17 (94 . 4 %) of 18 strains from healthy volunteers. PCR products of the cagA 39 variable region were obtained from 71 (92 . 2 %) of 77 Chinese H. pylori strains and could be classified into subgenotypes I, II and III, which gave PCR products of around 825, 900 and 950 bp, respectively. Subgenotype I cagA predominated in Chinese H. pylori strains (67/71), whereas subgenotype II cagA presented in two isolates from patients with chronic gastritis and subgenotype III presented in two isolates from healthy volunteers. Therefore, neither cagA nor its 39 region variants can be used as a sole marker for the presence of particular H. pylori-related gastroduodenal diseases in the Chinese population. INTRODUCTIONIt is generally accepted that Helicobacter pylori strains that produce CagA, an antigenic outer-membrane protein of variable molecular mass from 128 to 140 kDa, are highly virulent. Several studies have implicated CagA in the development of duodenal ulcer and gastric adenocarcinoma in most western populations (Covacci et al., 1993;Blaser et al., 1995;Torres et al., 1998;Bach et al., 1999;Miehlke et al., 2000;Abasiyanik et al., 2002; Nomura et al., 2002a, b;Oliveira et al., 2003;Wu et al., 2003), whereas the majority of reports from east Asian countries (Miehlke et al., 1996; Pan et al., 1997; Shimoyama et al., 1997;Maeda et al., 1998;Zheng et al., 2000;Groves et al., 2002; Lai et al., 2002), as well as those from Estonia (Andreson et al., 2002) and Argentina (Catalano et al., 2001), showed a very high prevalence of cagA-positive H. pylori, irrespective of clinical manifestation. Furthermore, it was reported that a PCR primer set that amplified cagA from H. pylori isolated in one country failed to detect cagA in isolates from another country (Miehlke et al., 1996; Pan et al., 1997). These discrepancies have led to the hypotheses that there may be several distinct forms of CagA with an uneven geographical distribution, that differences in cagA subgenotypes may provide a marker for differences in virulence among cagA-positive H. pylori strains and that only some forms of CagA are associated with severe gastroduodenal diseases.cagA is noted for its sequence diversity, both within and outside the variable 39 region of the molecule. Repeat sequences in the variable region are the m...

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases

Zhou

Zhang

et al. 2004

Journal of Medical Microbiology

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“…In contrast to most Western studies, the role of harboring cagA+ H. pylori strains remains elusive in Chinese population. Past studies showed that virtually all Chinese strains are cagA+ and it is therefore not feasible to have further subgroup analysis (25,26). Moreover, most of our patients are suffering from noncardia cancer, which has a much stronger association with H. pylori infection.…”

Section: Discussionmentioning

confidence: 91%

Risk Factors Associated with the Development of Intestinal Metaplasia in First-Degree Relatives of Gastric Cancer Patients

Leung¹,

Ng²,

Chan

et al. 2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Family relatives of gastric cancer patients have a higher risk of gastric cancer and premalignant gastric lesions. We sought to determine the risk factors associated with the presence of intestinal metaplasia in a large cohort of gastric cancer relatives. First-degree relatives of gastric cancer patients were invited for screening gastroscopy. Endoscopic gastric biopsies were obtained from the antrum and corpus. Gastric biopsies were analyzed for Helicobacter pylori infection, severity of inflammation, and presence of intestinal metaplasia. Stepwise logistic regressions were used to identify for risk factors associated with presence of intestinal metaplasia in cancer relatives. Two hundred seventy cancer relatives underwent screening endoscopy (median age, 42; 47% male and 48% siblings). Among them, 161 (59.6%) were H. pylori positive and 81 (30%) had confirmed intestinal metaplasia. The following factors were found to be associated with the presence of intestinal metaplasia: age, male sex, H. pylori infection, birth order, alcohol use, siblings with stomach cancer, childhood living conditions, and water supply. Individuals with intestinal metaplasia had more severe acute and chronic inflammation in the antrum and corpus (P < 0.003). With multiple logistic regression, H. pylori infection [odds ratio (OR), 3.23], male gender (OR, 2.09), age (OR, 1.07), and a history of gastric cancer in siblings (OR, 1.91) were independent factors associated with the development of intestinal metaplasia in cancer relatives. In conclusion, we have identified risk factors associated with gastric intestinal metaplasia in stomach cancer relatives, which may be useful in the understanding of gastric carcinogenesis in these high-risk individuals. (Cancer Epidemiol Biomarkers Prev 2005; 14(12):2982 -6)

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“…Once infected, environmental influences, such as antioxidant levels in diet, have been suggested to affect progression of disease (14). Additional risk factors affecting the outcome of H. pylori infection include unexplained geographic differences in the incidence of gastric cancer even after controlling for factors, such as age at first exposure to H. pylori (15) and immunologic differences in the response to the chronic infection (16).…”

Section: Introductionmentioning

confidence: 99%

Intestinal Helminthiasis in Colombian Children Promotes a Th2 Response to Helicobacter pylori: Possible Implications for Gastric Carcinogenesis

Whary

Sundina

Bravo

et al. 2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Colombians living in coastal Tumaco have a lower incidence of Helicobacter pylori -associated gastric cancer compared with residents of Pasto in the high Andes. Considering the risk for H. pylori disease seems affected by features of bacterial virulence and host polymorphisms, other poorly understood influences, such as concurrent helminthiasis, may also be important. Methods: Fecal samples from 211 children were tested for parasites and sera from another cohort of 159 children and 92 adults were tested for IgE and H. pylori -specific IgG. Results: Most individuals (95%) from both areas were H. pylori seropositive, with a predominant response of IgG1 followed by IgG2 and low IgG3 and IgG4 antibodies. Compared with Pasto children, Tumaco children were more

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#85 Association of serum antibodies to the helicobacter pylori caga antigen with precancerous gastric lesions in chinese populations with contrasting gastric cancer rates

Cited by 8 publications

References 0 publications

cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases

cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases

Risk Factors Associated with the Development of Intestinal Metaplasia in First-Degree Relatives of Gastric Cancer Patients

Intestinal Helminthiasis in Colombian Children Promotes a Th2 Response to Helicobacter pylori: Possible Implications for Gastric Carcinogenesis

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