85 Effects of the Inclusion of Vitamin B2 By-Product on Growth Performance, Blood Profiles and Meat Quality in Growing-Finishing Pigs.

Shi, Huan; Kim, J K; Serpunja, Subin; Hu, Jing; Kim, I H

doi:10.1093/jas/sky073.083

Cited by 3 publications

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“…Further investigation is needed to explore the reasons for the low yield of Cas9 in the CTRL group. Furthermore, in the ABF group, several proteins with beneficial effects on host health appeared, including riboflavin and isoprenoid biosynthetic processes, tetrahydrofolate interconversion, and the carboxylic acid metabolic processes [29][30][31][32][33] (Table 2 and Supplementary Table 2). We propose that ABF pigs receive probiotics containing Lactobacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Metaproteomic analysis of gut resistome in the cecal microbiota of fattening pigs raised without antibiotics

Buthasane

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Improper use of antibiotics in swine could reduce commensal bacteria and possibly increase pathogen infections via the gut resistome. This study aimed to compare the metaproteomic profiles of gut resistome and related metabolism in the cecal microbiota of fattening pigs raised under antibiotic-free (ABF) conditions with those of ordinary industrial pigs (controls, CTRL).�The top three relative abundant microbials in both groups were Escherichia coli, Ruminococcus and Lactobacillus, followed by Bacteroides and Bifidobacterium. E. coli, Lactobacillus and Bacteroides were found to be increased in the CTRL group, whereas Ruminococcus and Clostridium were greater in the ABF group. The highest abundance of antibiotic resistance proteins (>10 log2 expression levels, ELs) was tetracycline resistance (TetR) and aminoglycoside resistance (AMGR) proteins found in Bacteroides with a significant increase in the CTRL group. High TetR (5.32 ELs) was found in Ruminococcus in the CTRL group although pigs in both groups have never received tetracycline, possibly reflecting the influence of environments in farms. In E. coli, AMGR and ?-Lactamase family were observed in both groups (3-6 ELs), whereas multidrug resistance proteins were significantly expressed in the CTRL group (~3 ELs). In the ABF group, CRISPR-associated endonucleases Cas1 and Cas9, functioned to defend against viruses, were markedly observed in Ruminococcus and Lactobacillus, respectively, with 8.6 and 4.15 ELs, respectively. In conclusion, this study demonstrated that CRISPR-associated endonucleases were markedly observed in the ABF group, whereas higher levels of TetR, AMGR and multidrug resistance proteins were markedly observed in dominant bacterial species in the CTRL group.

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Section: Discussionmentioning

confidence: 99%

Metaproteomic analysis of gut resistome in the cecal microbiota of fattening pigs raised without antibiotics

Buthasane

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Metaproteomic Analysis of Gut Resistome in the Cecal Microbiota of Fattening Pigs Raised without Antibiotics

Buthasane,

Roytrakul,

Phaonakrop

et al. 2023

Microbiol Spectr

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In order to control and reduce antibiotic use in animals, the Department of Livestock Development, Thailand, has launched a campaign for antibiotic-free livestock production. The present study has shown for the first time that CRISPR-associated endonucleases Cas1 and Cas9, which function to defend against viruses, were markedly observed in Ruminococcus and Lactobacillus , respectively, in ceca of pigs raised without antibiotics (ABF).

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Phenotypic features and analysis of genes supporting probiotic action unravel underlying perspectives of Bacillus velezensis VTX9 as a potential feed additive for swine

Quach

Nguyen

et al. 2021

Ann Microbiol

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Purpose To date, a total of 13 probiotic Bacillus species are considered as a Generally Recognized as Safe organism (GRAS) approved by the US Federal Food, Drug, and Cosmetic Act (FDCA), which are used for food and feed additives. However, Bacillus velezensis is not considered as a probiotic candidate in swine farming due to a lack of genetic basis of probiotic action-related traits. Therefore, the present study was undertaken to exploit the genetic basis underlying the probiotic traits of B. velezensis VTX9. Methods The genome sequencing of B. velezensis VTX9 was performed on a PacBio Sequel platform. The probiotic properties including biosafety, antioxidative capacity, and riboflavin and exopolysaccharide production were evaluated by using genotypic and phenotypic analysis. The secondary metabolite potentials were also predicted. Results Strain VTX9 isolated from swine feces proved some probiotic properties including resistance to 3 mM H2O2, 0.6 mM bile salt, low pH, and antipathogenic activity. The complete genome of B. velezensis VTX9 consists of a 3,985,800 bp chromosome that housed 3736 protein-coding genes and 5 plasmids with the size ranging from 7261 to 20,007 bp. Genome analysis revealed no functional genes encoding enterotoxins and transferable antibiotic resistance, which confirmed the safety of VTX9. A total of 82 genes involved in gastrointestinal stress tolerance were predicted, which has not been reported previously. The maximum production of riboflavin reached 769 ± 7.5 ng/ml in LB medium after 72 h, which was in agreement with the complete de novo riboflavin biosynthetic pathway exploited for the first time in the B. velezensis genome. Antagonistic activity against pathogenic bacteria was attributed to 10 secondary metabolites clusters. The presence of a large gene cluster involved in biosynthesis of exopolysaccharides underscored further the adhesion and biofilm-forming capabilities of VTX9 in swine intestines. Conclusion Our results revealed for the first time that B. velezensis VTX9 has the potential to be a probiotic candidate. The information provided here on the genome of B. velezensis VTX9 opens new opportunities for using B. velezensis as a feed additive for swine farming in the future.

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85 Effects of the Inclusion of Vitamin B2 By-Product on Growth Performance, Blood Profiles and Meat Quality in Growing-Finishing Pigs.

Cited by 3 publications

References 0 publications

Metaproteomic analysis of gut resistome in the cecal microbiota of fattening pigs raised without antibiotics

Metaproteomic analysis of gut resistome in the cecal microbiota of fattening pigs raised without antibiotics

Metaproteomic Analysis of Gut Resistome in the Cecal Microbiota of Fattening Pigs Raised without Antibiotics

Phenotypic features and analysis of genes supporting probiotic action unravel underlying perspectives of Bacillus velezensis VTX9 as a potential feed additive for swine

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