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Hanhineva, Kati; Kärenlampi, Sirpa

doi:10.1186/1472-6750-7-11

Cited by 27 publications

(3 citation statements)

References 44 publications

(54 reference statements)

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“…Fundamental research is imperative to propel transformation frequency in delivering genes and develop a highly efficient and stable regeneration system of strawberry, although great strides have already been achieved [ 3 , 5 , 6 , 8 ]. A. tumefaciens -mediated genetic transformation of strawberry had been hampered dramatically by their generally poor response to tissue culture and genotype dependence [ 36 ]. Time-consuming and low efficiencies have also impeded stable genetic transformation.…”

Section: Discussionmentioning

confidence: 99%

Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

Yan,

Ma,

et al. 2023

Plant Methods

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Background The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-mediated hairy root transformation is an alternative for exploring gene functions, especially the genes specifically expressed in roots. However, the hairy root transformation has not been established in strawberry. Results Here, we described an efficient and rapid hairy root transgenic system for strawberry using A. rhizogenes. Strain of A. rhizogenes MSU440 or C58C1 was the most suitable for hairy root transformation. The transformation efficiency was highest when tissues contained hypocotyls as explants. The optimal procedure involves A. rhizogenes at an optical density (OD600) of 0.7 for 10 min and co-cultivation duration for four days, achieving a transgenic efficiency of up to 71.43%. An auxin responsive promoter DR5ver2 carrying an enhanced green fluorescent protein (eGFP) marker was transformed by A. rhizogenes MSU440, thereby generating transgenic hairy roots capable of high eGFP expression in root tip and meristem of strawberry where auxin accumulated. Finally, this system was applied for functional analysis using jGCaMP7c, which could sense calcium signals. A significant upsurge in eGFP expression in the transgenic hairy roots was displayed after adding calcium chloride. The results suggested that this approach was feasible for studying specific promoters and could be a tool to analyze gene functions in the roots of strawberries. Conclusion We established a rapid and efficient hairy root transformation in strawberry by optimizing parameters, which was adequate for promoter analysis and functional characterization of candidate genes in strawberry and other rosaceous plants.

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Section: Discussionmentioning

confidence: 99%

Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

Yan,

Ma,

et al. 2023

Plant Methods

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“…This system was therefore adopted for selecting putatively transformed embryos, as an alternative to the conventional method of selection on semi-solid medium. Although the use of TIS for mass clonal propagation has increased (Etienne and Berthouly 2002;Watt 2012), reports of its application for the selection phase of genetic transformation studies are scarce and involve genetic transformation based on adventitious shoot regeneration in pineapple (Espinosa et al 2002) and strawberry (Hanhineva and Kärenlampi 2007), or adventitious root formation in sage (Marchev et al 2011). However, none of these studies compared the transformation efficiency of TIS and semi-solid medium.…”

Section: Discussionmentioning

confidence: 99%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

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As the limiting factor for recovery of plants from oak embryogenic lines is the low embryo conversion rate, axillary shoot lines were established from transformed germinated embryos.Transformed embryos and shoots were cultured in medium with or without kanamycin and the responses to several morphogenetic processes (recovery after cryopreservation, germination, shoot proliferation, and rooting) were evaluated.2

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“…In addition to improvements in the cell culture technologies for these species, there have been significant advances in the design of more efficient transformation constructs [101][102][103] for both biolistics [104,105] and Agrobacterium-based methods [106,107]. Similarly, recent improvements have been reported for banana [108], clementine [109], legumes [110], opium poppy [111] and strawberry [112]. There have also been some claimed improvements in the development of novel methods for direct gene transfer [113][114][115].…”

Section: Regeneration and Transformation Techniquesmentioning

confidence: 99%

Plant Cell Culture – Present and Future

Dunwell

2010

Plant Cell Culture

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Plant cell culture has a long history of development and exploitation that has been reviewed many times [1]. There are also several monographs and textbooks that provide detailed discussion on specific techniques and their application [2, 3]. By necessity therefore, the present review is limited in scope and, for the most part, will focus on recent publications. The various sections below each concentrate on a specific aspect of the technology; these are followed by a summary section relating to commercialization. MicropropagationMicropropagation in vitro is well established as a method to propagate [4], preserve and transport germplasm of many species including horticultural [5-7], medicinal [8, 9] and woody [10,11] plants. It also reduces the risk of moving pathogens and insects with the germplasm owing to the inherent pathogen detection capabilities of aseptic cultures. Since this technology is usually limited to the multiplication of pre-existing meristems and does not involve the regeneration of plants from single cells or tissue, it will not be discussed in detail here.

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Untitled

Cited by 27 publications

References 44 publications

Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Plant Cell Culture – Present and Future

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