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Sariya, Ladawan; Prempracha, Nalinee; Keelapang, Poonsook; Chittasophon, Nuanchan

doi:10.2306/scienceasia1513-1874.2006.32.083

Cited by 3 publications

(2 citation statements)

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“…Pertinently to our study, the 37 phages identified in the Burkholderia genomes were either myoviruses, Mu-like viruses, or siphoviruses. Although one previous study reported the presence of a putative podovirus in the genome of B. pseudomallei , there are no published genetic studies to support this work [7]. Another recent study isolated six B. pseudomallei myoviruses from soil in Thailand, these were morphologically characterised to have lytic properties [10].…”

Section: Resultsmentioning

confidence: 88%

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Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei

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Kritsiriwuthinan

Galyov

et al. 2011

Virol J

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Burkholderia pseudomallei is a saprophytic soil bacterium and the etiological agent that causes melioidosis. It is naturally resistant to many antibiotics and therefore is difficult to treat. Bacteriophages may provide an alternative source of treatment. We have isolated and characterised the bacteriophage ΦBp-AMP1. The phage is a member of the Podoviridae family and has a genome size of ~ 45 Kb. Molecular data based on the gene which encodes for the phage tail tubular protein suggests that the phage is distinct from known phages but related to phages which infect B. thailandensis and Ralstonia spp. The phage ΦBp-AMP1 is the first B. pseudomallei podovirus to be isolated from the environment rather than being induced from a bacterial culture. It has a broad host range within B. pseudomallei and can infect all 11 strains that we tested it on but not related Burkholderia species. It is heat stable for 8 h at 50°C but not stable at 60°C. It may potentially be a useful tool to treat or diagnose B. pseudomallei infections as it can lyse several strains of clinical relevance.

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Section: Resultsmentioning

confidence: 88%

“…Prophages have been shown to be abundant in B. pseudomallei genomes [7-9]. A recent study reported the abundance and diversity of prophages in B. pseudomallei , B. thailandensis and B. mallei genomes and suggested how they contribute to the phenotypic diversity in the Burkholderia species complex [8].…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei

Gatedee

Kritsiriwuthinan

Galyov

et al. 2011

Virol J

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Cloning, expression, and characterization of a peptidoglycan hydrolase from the Burkholderia pseudomallei phage ST79

et al. 2016

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The lytic phage ST79 of Burkholderia pseudomallei can lyse a broad range of its host including antibiotic resistant isolates from within using a set of proteins, holin, lysB, lysC and endolysin, a peptidoglycan (PG) hydrolase enzyme. The phage ST79 endolysin gene identified as peptidase M15A was cloned, expressed and purified to evaluate its potential to lyse pathogenic bacteria. The molecular size of the purified enzyme is approximately 18 kDa and the in silico study cited here indicated the presence of a zinc-binding domain predicted to be a member of the subfamily A of a metallopeptidase. Its activity, however, was reduced by the presence of Zn2+. When Escherichia coli PG was used as a substrate and subjected to digestion for 5 min with 3 μg/ml of enzyme, the peptidase M15A showed 2 times higher in lysis efficiency when compared to the commercial lysozyme. The enzyme works in a broad alkaligenic pH range of 7.5–9.0 and temperatures from 25 to 42 °C. The enzyme was able to lyse 18 Gram-negative bacteria in which the outer membrane was permeabilized by chloroform treatment. Interestingly, it also lysed Enterococcus sp., but not other Gram-positive bacteria. In general, endolysin cannot lyse Gram-negative bacteria from outside, however, the cationic amphipathic C-terminal in some endolysins showed permeability to Gram-negative outer membranes. Genetically engineered ST79 peptidase M15A that showed a broad spectrum against Gram-negative bacterial PG or, in combination with an antibiotic the same way as combined drug methodology, could facilitate an effective treatment of severe or antibiotic resistant cases.

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Experimental Phage Therapy for Burkholderia pseudomallei Infection

et al. 2016

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Burkholderia pseudomallei is an intracellular Gram-negative bacterial pathogen intrinsically resistant to a variety of antibiotics. Phages have been developed for use as an alternative treatment therapy, particularly for bacterial infections that do not respond to conventional antibiotics. In this study, we investigated the use of phages to treat cells infected with B. pseudomallei. Phage C34 isolated from seawater was purified and characterised on the basis of its host range and morphology using transmission electron microscopy (TEM). Phage C34 was able to lyse 39.5% of B. pseudomallei clinical strains. Due to the presence of contractile tail, phage C34 is classified as a member of the family Myoviridae, a tailed double-stranded DNA virus. When 2 × 105 A549 cells were exposed to 2 × 107 PFU of phage C34, 24 hours prior to infection with 2 × 106 CFU of B. pseudomallei, it was found that the survivability of the cells increased to 41.6 ± 6.8% as compared to 22.8 ± 6.0% in untreated control. Additionally, application of phage successfully rescued 33.3% of mice infected with B. pseudomallei and significantly reduced the bacterial load in the spleen of the phage-treated mice. These findings indicate that phage can be a potential antimicrobial agent for B. pseudomallei infections.

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Cited by 3 publications

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Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei

Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei

Cloning, expression, and characterization of a peptidoglycan hydrolase from the Burkholderia pseudomallei phage ST79

Experimental Phage Therapy for Burkholderia pseudomallei Infection

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