9-cis-epoxycarotenoid dioxygenase 1 confers heat stress tolerance in rice seedling plants

Zhang, Yijin; Liu, Xiong; Su, Rui; Xiao, Yunhua; Deng, Huabing; Lu, Xinya; Wang, Rui; Chen, Guihua; Tang, Wenbang; Zhang, Guilian

doi:10.3389/fpls.2022.1092630

Cited by 20 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the endogenous hormone content of plants is also closely related to oxidative stress mechanisms. Recent studies have shown that OsNCED1 reduces membrane damage and ROS levels in plants under heat stress by regulating ABA content [ 43 ]. Therefore, we also examined the transcript levels of CK-responsive genes in the KT and control groups under heat stress; these genes can be used as markers to evaluate the cytokinin response.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Kinetin Modulates ROS Homeostasis to Affect Heat Tolerance in Rice Seedlings

Mei

Chen

Wang

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Heat stress caused by rapidly changing climate warming has become a serious threat to crop growth worldwide. Exogenous cytokinin (CK) kinetin (KT) has been shown to have positive effects in improving salt and drought tolerance in plants. However, the mechanism of KT in heat tolerance in rice is poorly understood. Here, we found that exogenously adequate application of KT improved the heat stress tolerance of rice seedlings, with the best effect observed when the application concentration was 10−9 M. In addition, exogenous application of 10−9 M KT promoted the expression of CK-responsive OsRR genes, reduced membrane damage and reactive oxygen species (ROS) accumulation in rice, and increased the activity of antioxidant enzymes. Meanwhile, exogenous 10−9 M KT treatment significantly enhanced the expression of antioxidant enzymes, heat activation, and defense-related genes. In conclusion, exogenous KT treatment regulates heat tolerance in rice seedlings by modulating the dynamic balance of ROS in plants under heat stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Exogenous Kinetin Modulates ROS Homeostasis to Affect Heat Tolerance in Rice Seedlings

Mei

Chen

Wang

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The software to acquire raw data is LightCycler ® QC Test Software 3.0. The primers for the internal reference gene OsActin and the target genes for real-time quantitative PCR are shown in Supplementary Table S1 [ 40 , 54 , 55 ]. The above experiments were carried out in three independent biological replicates, and each biological replicate was with three technical replicates to analyze the expression level of the target genes by the 2 −ΔΔCt method [ 56 ].…”

Section: Methodsmentioning

confidence: 99%

Knockout of the Chlorophyll a Oxygenase Gene OsCAO1 Reduces Chilling Tolerance in Rice Seedlings

Xiong,

Wen,

Song

et al. 2024

Genes

Self Cite

View full text Add to dashboard Cite

Chilling stress is one of the main abiotic factors affecting rice growth and yield. In rice, chlorophyllide a oxygenase encoded by OsCAO1 is responsible for converting chlorophyllide a to chlorophyllide b, playing a crucial role in photosynthesis and thus rice growth. However, little is known about the function of OsCAO1 in chilling stress responses. The presence of the cis-acting element involved in low-temperature responsiveness (LTR) in the OsCAO1 promoter implied that OsCAO1 probably is a cold-responsive gene. The gene expression level of OsCAO1 was usually inhibited by low temperatures during the day and promoted by low temperatures at night. The OsCAO1 knockout mutants generated by the CRISPR-Cas9 technology in rice (Oryza sativa L.) exhibited significantly weakened chilling tolerance at the seedling stage. OsCAO1 dysfunction led to the accumulation of reactive oxygen species and malondialdehyde, an increase in relative electrolyte leakage, and a reduction in antioxidant gene expression under chilling stress. In addition, the functional deficiency of OsCAO1 resulted in more severe damage to chloroplast morphology, such as abnormal grana thylakoid stacking, caused by low temperatures. Moreover, the rice yield was reduced in OsCAO1 knockout mutants. Therefore, the elevated expression of OsCAO1 probably has the potential to increase both rice yield and chilling tolerance simultaneously, providing a strategy to cultivate chilling-tolerant rice varieties with high yields.

show abstract

“…SOD and POD activities were determined using the riboflavin nitro blue tetrazolium (NBT) method and the guaiacol method, respectively. Leaves were stained using 3,3-diaminobenzidine (DAB) and NBT as described previously [26]. H 2 O 2 and O 2…”

Section: Determination Of Reactive Oxygen Species and Antioxidant Enz...mentioning

confidence: 99%

GDSL Lipase Gene HTA1 Negatively Regulates Heat Tolerance in Rice Seedlings by Regulating Reactive Oxygen Species Accumulation

Su,

Luo,

Wang

et al. 2024

Antioxidants

Self Cite

View full text Add to dashboard Cite

High temperature is a significant environmental stress that limits plant growth and agricultural productivity. GDSL lipase is a hydrolytic enzyme with a conserved GDSL sequence at the N-terminus, which has various biological functions, such as participating in plant growth, development, lipid metabolism, and stress resistance. However, little is known about the function of the GDSL lipase gene in the heat tolerance of rice. Here, we characterized a lipase family protein coding gene HTA1, which was significantly induced by high temperature in rice. Rice seedlings in which the mutant hta1 was knocked out showed enhanced heat tolerance, whereas the overexpressing HTA1 showed more sensitivity to heat stress. Under heat stress, hta1 could reduce plant membrane damage and reactive oxygen species (ROS) levels and elevate the activity of antioxidant enzymes. Moreover, real-time quantitative PCR (RT-qPCR) analysis showed that mutant hta1 significantly activated gene expression in antioxidant enzymes, heat response, and defense. In conclusion, our results suggest that HTA1 negatively regulates heat stress tolerance by modulating the ROS accumulation and the expression of heat-responsive and defense-related genes in rice seedlings. This research will provide a valuable resource for utilizing HTA1 to improve crop heat tolerance.

show abstract

9-cis-epoxycarotenoid dioxygenase 1 confers heat stress tolerance in rice seedling plants

Cited by 20 publications

References 53 publications

Exogenous Kinetin Modulates ROS Homeostasis to Affect Heat Tolerance in Rice Seedlings

Exogenous Kinetin Modulates ROS Homeostasis to Affect Heat Tolerance in Rice Seedlings

Knockout of the Chlorophyll a Oxygenase Gene OsCAO1 Reduces Chilling Tolerance in Rice Seedlings

GDSL Lipase Gene HTA1 Negatively Regulates Heat Tolerance in Rice Seedlings by Regulating Reactive Oxygen Species Accumulation

Contact Info

Product

Resources

About