9. Effects of Low-Intensity Pulsed Ultrasound (LIPUS) on Osteoclasts and Osteoblasts: Analysis Using an Assay System With Fish Scale as a Model of Bone

Suzuki, Nobuo; Hanmoto, Taizo; Ikegame, Mika; Furusawa, Yukihiro; Tabuchi, Yoshiaki; Kondo, Takashi; Kitamura, Kei-ichiro; Sekiguchi, Toshio; Mikuni-Takagaki, Yuko; Hattori, Atsuhiko

doi:10.1097/01.bot.0000489982.40329.52

Cited by 7 publications

(4 citation statements)

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“…75 In addition, LIPUS could stimulate programmed cell death in osteoclasts, such as the higher number of apoptotic osteoclasts in goldfish scales representing apoptosis. 75 Moreover, LIPUS can inhibit osteoclast activity by increasing OPG and RANKL expression in osteoblasts. 76 Meng et al showed that LIPUS treatment suppressed RANKL-induced osteoclast differentiation through ERK signaling.ERK and downstream components of the c-Fos/NFATc1 cascade, such as TRAP, CTSK, ERK1/2, extracellular signal-regulated kinase 1/2; CCN2, cellular communication network factor 2.…”

Section: Osteoclastsmentioning

confidence: 99%

“…Osteoclasts partake in bone resorption, and LIPUS has been recently reported to rectify osteoclastic activities in fracture healing. Nobuo Suzuki et al using zebrafish and goldfish scales found that LIPUS could decrease the expression of osteoclastic markers while increasing osteoblastic markers 75 . In addition, LIPUS could stimulate programmed cell death in osteoclasts, such as the higher number of apoptotic osteoclasts in goldfish scales representing apoptosis 75 .…”

Section: Mechanism Of Lipus On Fracture Healingmentioning

confidence: 99%

“…Nobuo Suzuki et al using zebrafish and goldfish scales found that LIPUS could decrease the expression of osteoclastic markers while increasing osteoblastic markers 75 . In addition, LIPUS could stimulate programmed cell death in osteoclasts, such as the higher number of apoptotic osteoclasts in goldfish scales representing apoptosis 75 . Moreover, LIPUS can inhibit osteoclast activity by increasing OPG and RANKL expression in osteoblasts 76 .…”

Section: Mechanism Of Lipus On Fracture Healingmentioning

confidence: 99%

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Latest Progress of LIPUS in Fracture Healing

Guo,

Lv,

Lin

et al. 2024

J of Ultrasound Medicine

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The use of low‐intensity pulsed ultrasound (LIPUS) for promoting fracture healing has been Food and Drug Administration (FDA)‐approved since 1994 due to largely its non‐thermal effects of sound flow sound radiation force and so on. Numerous clinical and animal studies have shown that LIPUS can accelerate the healing of fresh fractures, nonunions, and delayed unions in pulse mode regardless of LIPUS devices or circumstantial factors. Rare clinical studies show limitations of LIPUS for treating fractures with intramedullary nail fixation or low patient compliance. The biological effect is achieved by regulating various cellular behaviors involving mesenchymal stem/stromal cells (MSCs), osteoblasts, chondrocytes, and osteoclasts and with dose dependency on LIPUS intensity and time. Specifically, LIPUS promotes the osteogenic differentiation of MSCs through the ROCK‐Cot/Tpl2‐MEK–ERK signaling. Osteoblasts, in turn, respond to the mechanical signal of LIPUS through integrin, angiotensin type 1 (AT1), and PIEZO1 mechano‐receptors, leading to the production of inflammatory factors such as COX‐2, MCP‐1, and MIP‐1β fracture repair. LIPUS also induces CCN2 expression in chondrocytes thereby coordinating bone regeneration. Finally, LIPUS suppresses osteoclast differentiation and gene expression by interfering with the ERK/c‐Fos/NFATc1 cascade. This mini‐review revisits the known effects and mechanisms of LIPUS on bone fracture healing and strengthens the need for further investigation into the underlying mechanisms.

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Section: Osteoclastsmentioning

confidence: 99%

Section: Mechanism Of Lipus On Fracture Healingmentioning

confidence: 99%

Section: Mechanism Of Lipus On Fracture Healingmentioning

confidence: 99%

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Latest Progress of LIPUS in Fracture Healing

Guo,

Lv,

Lin

et al. 2024

J of Ultrasound Medicine

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“…Preclinical trials have demonstrated the potential of LIPUS in the field of bone tissue repair and regeneration. LIPUS not only acts on osteoblasts [ 8 ], osteoclasts [ 9 ] and mesenchymal stem cells [ 10 ] to exert osteogenic effects, but also has a positive effect on bone healing and regeneration through its effects on blood vessels and nerves [ 11 , 12 ]. Ultrasonic lipid microbubbles (MBs) can burst to form acoustic cavitation under the action of ultrasound, and the energy released can start or promote the sonochemical reaction [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds

et al. 2023

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Background To investigate the effect of low-intensity pulsed ultrasound (LIPUS) combined with lipid microbubbles on the proliferation and bone regeneration of bone marrow mesenchymal stem cells (BMSCs) in poly (lactic-glycolic acid copolymer) (PLGA)/α-tricalcium phosphate (TCP) 3D-printed scaffolds. Methods BMSCs were irradiated with different LIPUS parameters and microbubble concentrations, and the best acoustic excitation parameters were selected. The expression of type I collagen and the activity of alkaline phosphatase were detected. Alizarin red staining was used to evaluate the calcium salt production during osteogenic differentiation. Results BMSCs proliferation was the most significant under the condition of 0.5% (v/v) lipid microbubble concentration, 2.0 MHz frequency, 0.3 W/cm2 sound intensity and 20% duty cycle. After 14 days, the type I collagen expression and alkaline phosphatase activity in the scaffold increased significantly compared to those in the control group, and alizarin red staining showed more calcium salt production during osteogenic differentiation. After 21 days, scanning electron microscopy experiments showed that osteogenesis was obvious in the PLGA/TCP scaffolds. Conclusion LIPUS combined with lipid microbubbles on PLGA/TCP scaffolds can promote BMSCs growth and bone differentiation, which is expected to provide a new and effective method for the treatment of bone regeneration in tissue engineering.

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Low‐intensity pulsed ultrasound protects subchondral bone in rabbit temporomandibular joint osteoarthritis by suppressing TGF‐β1/Smad3 pathway

Liu

et al. 2020

Journal Orthopaedic Research

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Transforming growth factor β1(TGF-β1)/Smad3 pathway promotes the pathological progression of subchondral bone in osteoarthritis. The aim of this study is to determine the effect of low-intensity pulsed ultrasound (LIPUS) on the pathological progression and TGF-β1/Smad3 pathway of subchondral bone in temporomandibular joint osteoarthritis (TMJOA). Rabbit TMJOA model was established by type II collagenase induction. The left joint in this model was continuously stimulated with LIPUS for 3 and 6 weeks (1 MHz; 30 mW/cm 2) for 20 min/day. The morphological and histological features of subchondral bone were respectively examined by microcomputed tomography and Safranin-O staining. The number of osteoclasts was quantitatively assessed by tartrate-resistant acid phosphatase staining. Immunohistochemistry and Western blot analysis were conducted to evaluate the protein expression of Cathepsin K and TGF-β1/Smad3 pathway. The results indicated that LIPUS could improve the trabecular microstructure and histological characteristics of subchondral bone in rabbit TMJOA. It also suppressed abnormal subchondral bone resorption and activation of TGF-β1/Smad3 pathway, characterized by the number of osteoclasts, protein expression levels of Cathepsin K, TGF-β1, type II TGFβ receptor, and phosphorylated Smad3 (pSmad3) were decreased. In conclusion, LIPUS promoted the quality of subchondral bone by suppressing osteoclast activity and TGF-β1/Smad3 pathway in rabbit TMJOA.

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9. Effects of Low-Intensity Pulsed Ultrasound (LIPUS) on Osteoclasts and Osteoblasts: Analysis Using an Assay System With Fish Scale as a Model of Bone

Cited by 7 publications

References 0 publications

Latest Progress of LIPUS in Fracture Healing

Latest Progress of LIPUS in Fracture Healing

Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds

Low‐intensity pulsed ultrasound protects subchondral bone in rabbit temporomandibular joint osteoarthritis by suppressing TGF‐β1/Smad3 pathway

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