9‐oxo‐10(E),12(E)‐octadecadienoic acid derived from tomato is a potent PPAR α agonist to decrease triglyceride accumulation in mouse primary hepatocytes

Abstract

These findings suggest that tomatoes containing 9-oxo-ODA that acts on PPARα are valuable for ameliorating abnormalities of lipid metabolism.

Metabolomics reveal 1-palmitoyl lysophosphatidylcholine production by peroxisome proliferator-activated receptor α

“…Mouse hepatocytes were prepared as previously described ( 23 ). Briefl y, C57BL/6J male mice (wild type and PPAR ␣ Ϫ / Ϫ )…”

Comparative and Stability Analyses of 9- and 13-Oxo-octadecadienoic Acids in Various Species of Tomato

“…In our previous study, 9-Oxo-(10E,12E)-ODA, which has been identified as a PPAR agonist, 10) was detected in an extract of tomato fruit (sample 6, Furikoma in the present study) treated with heat and acid, but 9-Oxo-(10E,12Z)-ODA was detected in an intact extract in this study. Furthermore, the production processes for tomato juice include several steps with heating and acid treatment.…”

“…[7][8][9] Our previous study has indicated that an ingredient in tomato (brand name Furikoma) activates PPAR, and that the ingredient is 9-Oxo-(10E,12E)-octadecadienoic acid (9-Oxo-(10E,12E)-ODA) by nuclear magnetic resonance (NMR) and liquid chromatography/mass spectrometer (LC/MS). 10) Tomato is processed in many ways (fruit, juice, ketchup, and so on). In this study, we performed a comparative analysis of 9-oxo-ODA and its isomers using various species and processed foods of tomato fruits by ultra performance liquid chromatography (UPLC)/time of flight mass spectrometry (TOF-MS).…”

Effect of Dietary Phytol on the Expression of α-Amino-β-carboxymuconate-ε-semialdehyde Decarboxylase, a Key Enzyme of Tryptophan-niacin Metabolism, in Rats

“…Primary hepatocytes were obtained from the liver of a control rat (80-120 g) without any administration by using a collagenase perfusion method. 19) The isolated hepatocytes were resuspended in Dulbecco's modified Eagle's medium (DMEM; Nissui, Tokyo, Japan) supplemented with 20% (v/v) heat-inactivated fetal bovine serum (Biowest, Nuaille, France), 0.1 mM insulin and 1 mM dexamethasone, and cultured on type 1 collagen-coated 12-well plates (Asahi Glass Co., Tokyo, Japan) at a cell density of 2 Â 10 5 cells/mL. After 6 h of incubation at 37 C in a 5% CO 2 atmosphere, the medium was renewed to remove the unattached hepatocytes.…”