To determine the safety and efficacy of black tea extract in the treatment of bacterial conjunctivitis in a rabbit model and compare it with that of gatifloxacin drops. Methods: Black tea extract was tested in vitro on bacterial cultures of methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. Forty-two rabbit eyes were cultured with either MRSA (n¼21) or P. aeruginosa (n¼21) and further divided into a control group (n¼5), a tea group (n¼8) treated with black tea extract, and a gatifloxacin group (n¼8) treated with 0.3% gatifloxacin eye drops. Conjunctival swabs were collected on the third and fifth days. Results: The tea extract successfully inhibited the growth of both organisms at a concentration of 400 mg/mL. Rabbits in the treatment groups showed a reduction in the clinical index on day 2 (P,0.01), unlike the control group (P¼0.1), for both organisms. Resolution of conjunctivitis was achieved on days 4 and 5 in the tea and gatifloxacin groups, respectively. On days 3 and 5, while the control group still showed considerable bacterial growth, the tea and gatifloxacin groups showed its inhibition. Conclusion: Tea extract has antimicrobial effects similar to those of gatifloxacin in a rabbit model of conjunctivitis.