In this paper, a single-mode Q-switched 980 nm Yb-doped fiber amplifier were studied experimentally. In the experiment, we used a 980 nm acousto-optics (AO) Q-switched Yb-doped singlemode fiber laser as the seed source, which generated an average output power of 73 mW at 980 nm with the pulse width of 10 ns at repetition frequency of 16 kHz. Then, another single-mode Yb-doped fiber pumped by a cw 946 nm Nd:YAG laser composed the fiber amplifier. As a result, the 980 nm amplified light with 150 mW average output power was generated. Through frequency doubling the 980 nm laser with a BIBO crystal, an average output power of 8 mW of pulsed blue-green laser at 490 nm was generated.